Preparation method of pearl producing immune reinforcing agent
An immune enhancer and bead breeding technology, which is applied in the field of preparation of immune enhancer, can solve the problems of not very long-lasting action time and stress resistance of mother oyster, so as to improve yield and quality, increase nuclear retention rate, and reduce rejection The effect of the reaction
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Embodiment 1
[0034] Yuzhu immune enhancer
[0035] The composition and proportion of Yuzhu immune enhancer are as follows:
[0036] Zn 2+ Metal chelate 0.4g
[0037] Biological tissue fluid 15mL
[0038] Lecithin 0.3g
[0039] ATP 6mL
[0040] Penicillin 0.1g
[0041] CaCl 2 0.1g
[0042] KCl 0.02g
[0043] 0.4% normal saline 1000mL
[0044] The various solid components mentioned above are mixed according to appropriate proportions, fully dissolved with 0.4% normal saline, then ATP, antibiotic solution and biological tissue fluid are added, and finally 0.4% normal saline is used for constant volume.
Embodiment 2
[0046] Yuzhu immune enhancer
[0047] The composition and proportion of Yuzhu immune enhancer are as follows:
[0048] Zn 2+ Metal chelate 0.8g
[0049] Biological tissue fluid 30mL
[0050] Lecithin 6g
[0051] ATP 7mL
[0052] Streptomycin Sulfate 0.6g
[0053] CaCl 2 0.4g
[0054] KCl 0.08g
[0055] 0.4% normal saline 1000mL
Embodiment 3
[0057] At Xuwen Pearl Farm in Zhanjiang, artificial nucleation was carried out on Pinctada martensii, and two nuclei were implanted in each pearl oyster. Pearl-cultured immune enhancers were used during the nucleation process. Pearls were taken out from the pearl oysters that had been cultivated for 2 months. The rate of nucleation in the oysters reached 72.6%, and the number of nuclei was 1.45 per oyster. A nacre of about 0.1 mm had grown on the surface of the pearl nuclei.
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