Preventive agent or therapeutic agent for disease caused by abnormal bone metabolism
A technology for abnormal bone metabolism and preventive medicine, which can be used in bone diseases, anti-tumor drugs, drug combinations, etc., and can solve problems such as difficult to find better treatment methods or preventive methods.
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Embodiment 1
[0042] [Example 1] N-hydroxyl-4-{5-[4-(5-isopropyl-2-methyl-1,3-thiazol-4-yl)phenoxy]pentyloxy}-benzamidine Effects of and risedronic acid on the formation of osteoclast-like cells
[0043] As for the cells from mouse bone marrow, the bone marrow of mouse femur and tibia was collected, and then red blood cells were removed by conventional methods, and 4×10 5 The density of cells / well was seeded in a 96-well plate. After culturing overnight, 1α, 25-dihydroxyvitamin D3 (hereinafter referred to as 1α, 25(OH) 2 D. 3 ) medium to make 1α, 25(OH) 2 D. 3 reach 10 -9 Concentration of M, in 1α, 25(OH) 2 D. 3 In the presence of 7 days, multinucleated osteoclast-like cells were formed. As the medium, αMEM medium supplemented with 10% fetal calf serum (hereinafter referred to as 10% FCS-αMEM) was used. Addition of 1α,25(OH) to cells from mouse bone marrow 2 D. 3 , to the cell culture system from mouse bone marrow: add solvent only (comparative example 1-1), add N-hydroxy-4-{5-[4...
Embodiment 2
[0058] [Example 2] N-hydroxyl-4-{5-[4-(5-isopropyl-2-methyl-1,3-thiazol-4-yl)phenoxy]pentyloxy}-benzamidine Study on the Effect of And Risedronic Acid on Osteoclast Formation
[0059] In addition to making N-hydroxy-4-{5-[4-(5-isopropyl-2-methyl-1,3-thiazol-4-yl)phenoxy]pentyloxy}-benzamidine in the medium Concentration in 10 -6 Except for M, the same experiment as in Example 1 was performed, and the following groups were set.
Embodiment 3
[0071] [Example 3] N-hydroxyl-4-{5-[4-(5-isopropyl-2-methyl-1,3-thiazol-4-yl)phenoxy]pentyloxy}-benzamidine Study on the effect of and clodronic acid on the formation of osteoclasts
[0072] In embodiment 1, except making clodronic acid in comparative example, embodiment with 10 -5 The concentration of M interacts with the cells to replace risedronic acid at 3×10 -7 Except for the concentration of M and the effect on cells, the same experiment as in Example 1 was carried out, and the following groups were set.
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