Rapid breeding method of senecio cruentus

A cineraria, fast technology, applied in the field of plant tissue culture, can solve the problems of unfavorable in vitro tissue regeneration large-scale culture, low germination rate of somatic embryos, poor stability of regeneration system, etc. The effect of high planting survival rate and stable regeneration system

Inactive Publication Date: 2010-08-25
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The germination rate of somatic embryos obtained by the direct induction method is low, and due to the lack of nutrition or the accumulation of toxic metabolites in the medium, the explants will stop growing, or even age, blacken and die, thereby reducing the regeneration rate of the explants. It is not conducive to the regeneration of isolated tissues and large-scale culture
[0010] Yu Xiaoying and others from Hunan Agricultural University carried out in vitro culture and rapid propagation research on the single-bud stem segments of 5 different flower and color mother plants of Cineraria as explants. The results showed that: 1) the explants from the mother plant M1 The induction rate of the plant in the A5 medium containing 0.1mg/LNAA+2mg/LBA is the highest, and the buds germinate early and the number is large; 2) In the subculture, within the range of 0-2.0mg/L, with the concentratio

Method used

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  • Rapid breeding method of senecio cruentus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1. Test materials

[0051] 1. Mature cineraria ‘clown’ seeds come from the US Gold Smith company.

[0052] The five color varieties of Cineraria'clown' used in the embodiment of the present invention are blue, white, pink, scarlet and yellow.

[0053] 2. Plant growth regulator

[0054] The plant growth regulators used in the present invention adopt domestic indole butyric acid (IBA), naphthaleneacetic acid (NAA), 6-benzylamino adenine (6-BA) and 2,4-dichlorophenoxyacetic acid (2 , 4-D).

[0055] 3. Preparation of culture medium:

[0056] (1) The composition or preparation method of "MS basic medium";

[0057] Table 1 MS medium (Murashige and Skoog, 1962)

[0058] MS large elements

Mother liquor composition

content

(g / L)

MS trace elements

Mother liquor composition

content

(g / L)

Organic compound

Mother liquor composition

content

(g / L)

NH4NO3

33

H 3 BO 3

1.24

Inositol

20

KNO 3

38

ZnSO 4 ·7H 2 O

1.72

niacin

0.1

CaCl 2 ·2H 2 O

8.8

MnSO 4 ·4H ...

Embodiment 2

[0093] In addition to aseptic seedling culture, the seeds of scarlet cineraria "clown" were used; the 6-BA used in the clumping bud induction medium was 0.5 mg / L and the NAA was 0 mg / L; the 6-BA used in the callus induction medium was 2.0mg / L, NAA is 3.0mg / L, 2,4-D is 0.5mg / L; 6-BA used in callus proliferation medium is 0.5mg / L, NAA is 0.5mg / L; adventitious bud differentiation The 6-BA used in the medium is 2.0 mg / L, NAA is 3.0 mg / L, and 2,4-D is 0.5 mg / L; the IBA used in the adventitious rooting medium is 0.1 mg / L, all others are the same Example 1 is the same.

Embodiment 3

[0095] In addition to aseptic seedling culture, the seeds of blue cineraria "clown" are used; the 6-BA used in the clumping bud induction medium is 1.0 mg / L and the NAA is 0.1 mg / L; the 6-BA used in the callus induction medium 3.0mg / L, NAA 2.0mg / L, 2,4-D 0.5mg / L; 6-BA used in callus proliferation medium is 1.0mg / L, NAA is 0.5mg / L; adventitious buds The differentiation medium used 6-BA is 3.0mg / L, NAA is 2.0mg / L, 2,4-D is 0.5mg / L; the adventitious rooting medium used NAA is 0.1mg / L, the others are the same Example 1 is the same.

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Abstract

The invention discloses a rapid breeding method of senecio cruentus, belonging to the cultivation field of plant tissues. The method comprises the following steps of: (1) serving senecio cruentus seeds as explants; (2) cultivating cluster buds by induction; (3) cultivating strong seedlings; (4) cultivating calluses by induction; (5) cultivating the calluses by enrichment; (6) cultivating adventitious buds by differentiation; (7) cultivating adventitious roots by induction; and (8) hardening and transplanting seedlings, wherein a minimal medium adopted in each cultivation stage is an MS medium, and a growth regulating agent added in each cultivation stage is selected with optimum dosage and proportioning ratio after being tested by a orthogonal design. The senecio cruentus bred by the method has the advantages of strongly grown test-tube seedlings, high breeding coefficient and scale and industrial production, the average induced rooting rate reaches 94.68%, and the survival rate of transplantation reaches 100%.

Description

Technical field [0001] The invention relates to a plant tissue culture method, in particular to a method for rapid propagation of cineraria (Senecio cruentus) seeds in vitro, and belongs to the field of plant tissue culture. Background technique [0002] Cineraria (Senecio cruentus), also known as calamus and cineraria, is a perennial herb of the genus Senecio of the Compositea family (Compositea). The whole plant is densely pilose. The leaves form a triangle every three leaves. Cucumber leaves, hence the name Cineraria. It is native to the Canary Islands, Spain. It likes cold in nature, is intolerant to high temperature and frost, and is very fat. It likes loose and well-drained soil. The suitable growth temperature is 10℃~20℃, higher than 25℃, lower than 10℃, growth is slow, below 0℃, it is very easy to suffer from freezing damage. [0003] The cineraria is densely clustered. When in full bloom, the flowers cover the whole plant, the top of the flower grows, and most of the flo...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 戴思兰裴红美
Owner BEIJING FORESTRY UNIVERSITY
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