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Purification method of phloretin powder

A purification method and technology of phloretin are applied in the directions of skin care preparations, separation/purification of carbonyl compounds, cosmetics, etc., and can solve the problems of high cost, difficulty in industrialized large-scale production, etc., and achieve low production cost, easy industrialized production, The effect of reducing the extraction efficiency

Active Publication Date: 2013-06-05
上海利盛生化有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this technology needs to use relatively expensive phlorizin and hydrolase as raw materials, which has high cost and is not easy for industrialized large-scale production

Method used

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  • Purification method of phloretin powder

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take 10 kg of lychee shells, put them in a multi-functional extraction tank, add 100 liters of ethanol solution with a mass concentration of 30%, heat to 100°C, stir and extract for 0.5 hours, and filter to obtain 82 liters of crude extract. The crude extract was concentrated to 8.2 liters by a rotary evaporator, left to cool for 12 hours, and then centrifuged to remove the lower precipitate to obtain 7 liters of a concentrated solution. The concentrated solution was passed through a JK206 homogeneous strong basic anion exchange resin column, and eluted with HCl with a molar mass of 0.1M / L. The collected eluate passed through the HZ-816 macroporous non-polar adsorption resin column and was eluted with 20% ethanol. A total of 21 liters of the eluate was collected, concentrated by a rotary evaporator, and then dried by centrifugal spraying to produce 40 grams of phloretin powder. The content of phloretin was 80.5% as detected by HPLC.

Embodiment 2

[0025] Take 10 kg of lychee shells, put them in a multi-functional extraction tank, add 80 liters of ethanol solution with a mass concentration of 95%, heat to 40°C, stir and extract for 4 hours, and obtain 74 liters of crude extract after filtration. The crude extract was concentrated to 15.8 liters by a rotary evaporator, left to cool for 12 hours, and then centrifuged to remove the lower precipitate to obtain 12 liters of a concentrated solution. The concentrated solution was passed through a JK206 homogeneously porous strongly basic anion exchange resin column, and eluted with HCl with a molar mass of 0.5M / L. The collected eluate passed through the HZ-816 macroporous non-polar adsorption resin column and was eluted with 60% ethanol. A total of 23 liters of the eluate was collected, concentrated by a rotary evaporator, and then dried by centrifugal spraying to prepare 60 grams of phloretin powder.

Embodiment 3

[0027] Accurately weigh about 20 mg of the extract powder in Example 2, put it in a 50 ml brown volumetric flask, add methanol, dissolve it by ultrasonic, let cool to room temperature, dilute to volume, and shake well. Accurately draw 2ml, put it in a 10ml brown volumetric flask, dilute with methanol to constant volume, pass through a 0.45um filter membrane, and inject 5ul. Detection conditions: mobile phase: acetonitrile: 0.1% phosphoric acid water = 30:70 (V:V); detection wavelength: 280nm; flow rate: 1.0ml / min; chromatographic column: C18, 250×4.6mm, 5ul. The content of phloretin was detected by HPLC to be 95.2%.

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Abstract

The invention relates to a purification method of phloretin powder, belonging to the technical field of the chemical industry. The purification method comprises the following steps of: mixing and purifying leechee shells and an alcohol solution to obtain a crude extract; carrying out ion exchange resin separation after concentration; and finally carrying out macroporous resin separation to obtainthe phloretin powder. The invention has the advantages that the process is simple, the extraction ratio achieves above 60%, the purity of the extracted phloretin can achieve above 95%, and the extracted phloretin is applied to the fields of cosmetics.

Description

technical field [0001] The invention relates to a purification method in the technical field of chemical industry, in particular to a purification method of phloretin powder. Background technique [0002] Phloretin is a natural skin whitening agent newly researched and developed at home and abroad. It gets its name because it mainly exists in the rhizomes and root barks of plants such as apples and pears. Cosmetics with whitening effect are currently one of the most popular cosmetics, especially in Asia, and products with natural whitening ingredients are the most popular. Therefore, the development of phloretin has received increasing attention internationally. Phloretin is a new type of whitening agent that is increasingly valued internationally. Its whitening mechanism is because it can competitively inhibit the activity of tyrosinase in skin melanocytes, thereby interfering with the synthesis of melanin, and its inhibition rate is better than that of many current whiten...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C49/83C07C45/78A61K8/35A61Q19/02
Inventor 杨文朱卫吴小惠李新华
Owner 上海利盛生化有限公司
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