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Fermentation production method of fusidic acid

A production method and technology of fusidic acid are applied in the field of fermentation production of high-yield Staphylococcus antibiotic fusidic acid, can solve problems such as few research reports and few clinical applications, and achieve the effect of simple operation

Inactive Publication Date: 2010-08-25
JIANGSU JIUYANG BIOLOGICAL PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although fusidic acid has been widely used abroad for many years, it has less clinical application in China
In China, there are relatively few reports on the production of fusidic acid, and there are even fewer research reports on the production of fusidic acid by fermentation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Wash the spores of Fusidella spp from the PDA slant with physiological saline, make a spore suspension and inoculate it into a 250mL shaker flask with 50mL seed medium, and the inoculum size is 10 6 / mL. Be that temperature is 26 ℃, shaking culture in the shaker of rotating speed 200r / min 48 hours is the shake flask seed culture liquid; Receive in the 50L first-class seed tank with the inoculation amount of 5% (v / v), 26 ℃, Stir and ventilate at 0.4-0.5vvm, cultivate for 60hrs; then transfer to a 500L secondary seed tank, at 26°C, stir and ventilate at 0.4-0.5vvm, cultivate for 48hrs; obtain fermented seed liquid.

[0024] Clean the fermenter, assemble the temperature and pH detectors, dissolve the fermentation medium in water, pour it into the fermenter, and sterilize it at high temperature. During the sterilization process, correct the dissolved oxygen DO to 0%. Connect the air filter and other accessories after cooling.

[0025] The fermentation process of fusidic a...

Embodiment 2

[0029] Wash the spores of Fusidella spp from the PDA slant with physiological saline, make a spore suspension and inoculate it into a 250mL shaker flask with 50mL seed medium, and the inoculum size is 10 6 / mL. Be that temperature is 26 ℃, shaking culture in the shaker of rotating speed 200r / min 48 hours is the shake flask seed culture liquid; Receive in the 50L first-class seed tank with the inoculation amount of 5% (v / v), 26 ℃, Stir and ventilate at 0.4-0.5vvm, cultivate for 60hrs, then transfer to a 500L secondary seed tank, at 26°C, stir and ventilate at 0.4-0.5vvm, cultivate for 48hrs; obtain fermented seed liquid.

[0030] Clean the fermenter, assemble the temperature and pH detectors, dissolve the fermentation medium in water, pour it into the fermenter, pass it into the cooling water, and sterilize it. During the sterilization process, correct the dissolved oxygen DO to 0%. Connect the air filter and other accessories after cooling.

[0031] The fermentation process ...

Embodiment 3

[0035] Wash the spores of Fusidella spp from the PDA slant with physiological saline, make a spore suspension and inoculate it into a 250mL shaker flask with 50mL seed medium, and the inoculum size is 10 6 / mL. Be that temperature is 26 ℃, shaking culture in the shaker of rotating speed 200r / min 48 hours is the shake flask seed culture liquid; Receive in the 50L first-class seed tank with the inoculation amount of 5% (v / v), 26 ℃, Stir and ventilate at 0.4-0.5vvm, cultivate for 60hrs, then transfer to a 500L secondary seed tank, at 26°C, stir and ventilate at 0.4-0.5vvm, cultivate for 48hrs; obtain fermented seed liquid.

[0036] Clean the fermenter, assemble the temperature and pH detectors, dissolve the fermentation medium in water, pour it into the fermenter, pass it into the cooling water, and sterilize it. During the sterilization process, correct the dissolved oxygen DO to 0%. Connect the air filter and other accessories after cooling.

[0037] The fermentation process o...

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PUM

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Abstract

The invention discloses a fermentation production method of fusidic acid, which belongs to the technical field of fermentation engineering. The method comprises the stages of seed culture, preparation before inoculation, inoculation, multi-stage dissolved oxygen control and batch material supplementing fermentation. Fucidin can be produced through multi-stage fermentation, so the valence of the secondary metabolite of fusidic acid of the strain is greatly improved. The invention has the advantages of simple operation and low cost, and realizes the biological method production of antibiotics of the fusidic acid, in addition, target products of the fusidic acid obtained through the fermentation have high valence, and the invention is favorable for industrial production.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, and in particular relates to a fermentation production method of high-yield Staphylococcus antibiotic fusidic acid. Background technique [0002] Fusidic acid (Fusidic acid, FA), also known as fusidic acid, is a typical high-efficiency antibiotic. It is a narrow-spectrum antibiotic produced by Fusidia spp. It mainly has strong antibacterial activity against Gram-positive bacteria, especially Staphylococcus, including Staphylococcus aureus, coagulase-negative bacteria, Staphylococcus epiphysis, Clostridium Bacillus and coryneform bacteria have high sensitivity [Collignon P, Turndge J. International journal of antimicrobial agents, 1999, 12: 45-58]. In 1962, the Danish Leo Pharmaceutical Company first reported the discovery and extraction of fusidic acid from the fermentation broth of Fusidium coccineum. Fusidic acid has been widely used abroad for more than 40 years, but it stil...

Claims

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Application Information

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IPC IPC(8): C12P33/00C12R1/645
Inventor 纪晓俊丛蕾蕾李军胡燚苏永明
Owner JIANGSU JIUYANG BIOLOGICAL PHARMA
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