Unlock instant, AI-driven research and patent intelligence for your innovation.

Gold nanoparticles used as medicament carrier and method for preparing same

A gold nanoparticle and gold nanoseed technology, which can be used in drug combination, drug delivery, pharmaceutical formulation, etc.

Inactive Publication Date: 2012-06-06
UNIV OF SCI & TECH OF CHINA
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Chemistry & Biology" magazine (Chemistry & Biology, 11:557) reported that the prodrug of platinum drugs is the prodrug of cisplatin cis, cis, trans-diamminodichlorodisuccinic acid platinum structures and their biological uses, but so far there have been no reports of loading them on gold nanoparticles for drug delivery applications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Gold nanoparticles used as medicament carrier and method for preparing same
  • Gold nanoparticles used as medicament carrier and method for preparing same
  • Gold nanoparticles used as medicament carrier and method for preparing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Preparation of CTAB-stabilized gold nanoparticles

[0037] Preparation of gold nanoparticles bound by membrane-penetrating peptide (refer to the method of "Chemical Materials" (Chemistry Material, 2003, 15: 1957)):

[0038] Preparation of gold nano seed solution: Add 0.25mL of 0.005M chloroauric acid to 5mL of 0.1M CTAB solution and mix well, the solution is brownish yellow; add 0.5mL of 0.01M NaBH 4 solution, stirred rapidly for 2 minutes, and the solution was light brown at this time; put it in a water bath at 30°C for 2 hours, and set aside;

[0039] Preparation of CTAB-stabilized gold nanoparticles: first mix 5mL 0.1M CTAB and 5mL 0.01M chloroauric acid in a 30°C water bath with a magnetic stirrer; then add 0.18mL of 0.01M silver nitrate and 0.2mL of 0.1M Ascorbic acid was mixed evenly with a vortex oscillator to obtain a colorless and transparent growth solution; finally, 0.01 mL of the above gold nano-seed solution was added, mixed with a vortex oscillator for...

Embodiment 2

[0057] 1. Preparation of CTAB-stabilized gold nanoparticles Preparation of seed solution: Add 0.75mL of 0.075M chloroauric acid to 6mL of 0.15M CTAB solution and mix evenly, the solution is brownish yellow; add 0.75mL of 0.15M NaBH 4 solution, stirred rapidly for 10 minutes, and the solution was light brown at this time; put it in a water bath at 40°C for 10 hours, and set aside;

[0058] Preparation of CTAB-stabilized gold nanoparticles: first mix 6mL of 0.15M CTAB and 6mL of 0.015M chloroauric acid in a 40°C water bath with a magnetic stirrer; then add 0.3mL of 0.015M silver nitrate and 0.3mL of 0.15M Mix ascorbic acid with a vortex oscillator to obtain a colorless and transparent growth solution; finally add 0.02mL of the above gold nano seed solution,

[0059] The gold nanoparticles were mixed with a vortex shaker for 30 seconds and then placed in a water bath at 40°C for 12 hours.

[0060] 2. Preparation of prodrug compound 1 or compound 2 or compound 3 or compound 4 or ...

Embodiment 3

[0075] 1. Preparation of CTAB-stabilized gold nanoparticles

[0076] Preparation of gold nano seed solution: Add 1mL of 0.01M chloroauric acid to 10mL of 0.2M CTAB solution and mix evenly, the solution is brownish yellow; add 1mL of 0.02M NaBH 4 solution, stirred rapidly for 20 minutes, and the solution was light brown at this time; put it in a water bath at 50°C for 20 hours, and set aside;

[0077] Preparation of CTAB-stabilized gold nanoparticles: first mix 10mL of 0.2M CTAB and 10mL of 0.02M chloroauric acid in a 50°C water bath with a magnetic stirrer; then add 0.5mL of 0.02M silver nitrate and 0.4mL of 0.2 M ascorbic acid was mixed evenly with a vortex oscillator to obtain a colorless and transparent growth solution; finally, 0.03 mL of the above gold nano-seed solution was added, mixed with a vortex oscillator for 50 seconds, and then placed in a 50°C water bath for 24 hours.

[0078] 2. Preparation of prodrug compound 1 or compound 2 or compound 3 or compound 4 or com...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses gold nanoparticles used as a medicament carrier and a method for preparing the same. The gold nanoparticles are characterized in that: membrane-penetrating peptides are combined on the surfaces of the gold nanoparticles through mercapto by forming covalent bonds, and the membrane-penetrating peptides are also combined with the carboxyl groups of pro-medicaments of platinum-based medicaments through amino by forming amido bonds; the membrane-penetrating peptide is a peptide fragment which has 8 to 20 amino acids including 1 cysteine and 5 to 19 arginines; the pro-medicaments of platinum-based medicaments are pro-medicaments of cisplatin, carboplatin, or oxaliplatin; the pro-medicaments of the cisplatin are selected from compounds 1,2 and 3; the pro-medicaments of the carboplatin are selected from compounds 4,5 and 6; and the pro-medicaments of oxaliplatin are selected from compounds 7,8 and 9. The gold nanoparticles connecting the platinum-based medicaments and the membrane-penetrating peptides have obviously-enhanced toxicity to tumor cells, and the gold nanoparticles not connected with the platinum-based medicaments are non-toxic. In the invention, a new route is opened up for the application of the gold nanoparticles in the biomedical field.

Description

technical field [0001] The invention belongs to the technical field of platinum drug carriers, and in particular relates to a gold nanoparticle drug carrier loaded with platinum drugs and a preparation method thereof. Background technique [0002] According to the article in "Journal of Biomedical Engineering" (2004, Volume 21, No. 3, Pages 495-497), a drug carrier refers to a drug carrier that can load a drug and change the way the drug enters the body and its distribution in the body. A system that controls the drug release rate and delivers the drug to the target organ can effectively reduce the side effects of the drug on normal tissues and improve the bioavailability of the drug. [0003] According to data released by the Chinese Medical Association in 2007, malignant tumors have become the number one killer among various diseases that endanger the lives of Chinese residents, and 2 million people in China die of tumors every year. Cisplatin (Cisplatin, CDDP) is a plati...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/48A61K33/24A61K31/282A61K31/194A61K9/14A61P35/00A61K47/42A61K47/02A61K47/52A61K47/64
CPCA61K47/48238A61K47/48861A61K47/6923A61K47/62
Inventor 刘扬中闵元增
Owner UNIV OF SCI & TECH OF CHINA