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Application of an indole and skatole degrading strain lpc24

A technology of LPC24, LPC24CCTCCNO, which is applied in the application field of indole and skatole degrading microorganisms, and can solve the problems of rare degradation and other reports

Inactive Publication Date: 2011-12-14
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although predecessors have done some research work on the degradation of mixed microorganisms, there are few reports on the degradation of indole or skatole by pure bacteria

Method used

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  • Application of an indole and skatole degrading strain lpc24
  • Application of an indole and skatole degrading strain lpc24
  • Application of an indole and skatole degrading strain lpc24

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] One, a screening method for indole and skatole degrading bacteria:

[0015] (1) Material preparation:

[0016] 1. The experimental wastewater (livestock and poultry breeding wastewater) was taken from the sewage outlet of the original pig farm in Ezhou City, Hubei Province, and the activated sludge was taken from the special wastewater treatment laboratory of the Environmental College of China University of Geosciences (Wuhan). In the anaerobic reactor of wastewater (the experimental wastewater in the anaerobic reactor was taken from the sewage outlet of the original pig farm in Hubei Province, Ezhou City, Hubei Province).

[0017] 2. Culture medium:

[0018] Liquid MSM medium (g / L): (NH 4 ) 2 SO 4 , 0.33; NaNO 3 , 0.85; K 2 HPO 4 , 0.79; KH 2 PO 4 , 0.20; CaCl 2 , 0.05; MgCl 2 , 0.50; FeCl 2 , 0.01, H 2 O 1000mL; pH 7.2; after autoclaving, filter-sterilized skatole and indole were used to make the concentration of skatole and indole in the liquid MSM mediu...

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Abstract

The invention relates to the application of an indole and skatole degrading microorganism. The application of an indole- and skatole-degrading strain LPC24 is characterized in that the application of the strain is Pseudomonas putida LPC24 CCTCC NO: M209099 applied to the treatment of indole- and skatole-containing wastewater. The specific treatment method is: pick a single bacterium colony of Pseudomonas putida (Psedomonas.putida) LPC24, cultivate it overnight in liquid MSM medium, and press the cultured Pseudomonas putida (Psedomonas.putida) LPC24: containing indole and The volume ratio of skatole wastewater (such as livestock and poultry breeding wastewater) is 0.5-2: 100. Take Pseudomonas putida (Psedomonas.putida) LPC24 and inoculate it in indole and skatole wastewater (such as livestock and poultry breeding wastewater). Cultivate at a constant temperature of -35°C, the pH value is 6.0-8.0, and react for 3-5 days. The invention has good ability to remove indole and skatole in livestock and poultry breeding wastewater.

Description

technical field [0001] The invention relates to the application of an indole and skatole degrading microorganism. Background technique [0002] Indole and skatole are the main heterocyclic aromatic hydrocarbons with malodorous odor in livestock and poultry farm wastewater. They are produced by the decomposition of tryptophan in animals. Both compounds are also found in wastewater from the production of medical supplies, pesticides, disinfectants, and agrochemicals. The large-scale production and wide application of these chemicals have resulted in the pollution of soil, surface water, and groundwater, posing serious threats to human and animal health. Indole and skatole can be absorbed into the blood by the human body and animals, and produce toxic intermediate products after biological activation, which will affect the production of serotonin in the blood, and even cause hemolysis. Studies have shown that the increase of estrone concentration in animals is directly relate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F103/20
Inventor 李平王焰新王艳红童蕾刘珩刘琨
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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