Enterobacter bacteria for efficiently degrading organophosphorus pesticides and culturing method thereof
A technology of organophosphorus pesticides and strains, applied in bacteria, chemical instruments and methods, microorganism-based methods, etc., can solve problems such as ecological balance imbalance, harm to beneficial insects and birds, environmental pollution, etc.
Inactive Publication Date: 2012-05-23
LANZHOU UNIVERSITY OF TECHNOLOGY
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Problems solved by technology
However, the toxicity of organophosphorus pesticides has two sides: on the one hand, it can effectively control or eliminate the hazards of agricultural and forestry diseases, insects and weeds, and improve the yield and quality of agricultural products; on the other hand, the use of pesticides also brings environmental pollution, Harm beneficial insects and birds, leading to ecological imbalance
At the same time, it also causes pesticide residues in agricultural products, which is harmful to human health.
Method used
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[0027] Below in conjunction with embodiment the present invention is described in further detail:
[0028] combine figure 1 The bacterium Enterobacter ludwigii of the genus Enterobacter ludwigii that efficiently degrades organophosphorus pesticides has a short rod shape, no spores, a size of 0.6-1.1×1.2-2.5 μm, and a single end with tufted flagella. combine figure 2 , 3 4. The strain is a Gram-negative, aerobic bacterium with an optimum growth temperature of 30-37°C and an optimum pH of 7-8. It is cultivated on blood agar medium for 48 hours to form colonies with a diameter of 1-2mm. It is round, light yellow, opaque, raised, with neat edges, moist and shiny surface. The Genbank accession number of the 16SrDNA of the strain is GQ380575, and it was deposited in the General Microbiology Center of the China Committee for the Collection of Microorganisms on June 8, 2009, and the preservation number is CGMCC NO.3092.
[0029] The described high-efficiency degradation organopho...
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Abstract
The invention provides enterobacter bacteria for efficiently degrading organophosphorus pesticides and a culturing method thereof. The enterobacter bacteria are characterized in that the main biological property of the enterobacter bacteria is Gram-negative, thalli are in a short-rod shape without spores, and the size is 0.6-1.1*1.2-2.5 mum; and the enterobacter bacteria have single-end overgrowing flagella and are aerobic bacteria, the optimum growing temperature is 30-37 DEG C, the optimum pH value is 7-8, the enterobacter bacteria form a bacterial colony with the diameter of 1-2 mm after being cultured on a blood agar medium for 48 h, and the bacterial colony is circular in shape and yellow in color, has no transparence, knurls, trim edge and moist and glossy surface. The Genbank loginnumber of the bacterial strain 16SrDNA is GQ380575. The tolerance concentration of the bacterial strain on parathion-methyl and Dursban can reach 5000 mg / L in an inorganic salt medium containing 1% of cane sugar, the tolerance concentration on dimethoate reaches 10000 mg / L, the tolerance capacity of the bacterial strain is furthest higher than that of agricultural dose used in agricultural production, the degradation rate for the organophosphorus pesticides in soil reaches above 90%, and the enterobacter bacteria solve the problem that pesticide residues in the agricultural production exceed the standard, and can be used for producing green and nuisanceless agricultural products.
Description
technical field [0001] The invention mainly relates to the degradation of organophosphorus pesticide residues by means of microorganisms, in particular to an Enterobacter ludwigii bacterium of the genus Enterobacter ludwigii which efficiently degrades organophosphorus pesticides and its cultivation method, which is suitable for the production and processing of green and pollution-free agricultural products in modern agricultural production. Background technique [0002] Since Bayer Company of Germany first discovered that organic phosphorus has insecticidal properties and produced the first insecticide "1605-parathion" in 1944, organophosphorus has been widely used as a class of high-efficiency and broad-spectrum insecticides. Increased the yield of crops to a great extent. However, the toxic effect of organophosphorus pesticides has two sides: on the one hand, it can effectively control or eliminate the hazards of diseases, insects and weeds in agriculture and forestry, and...
Claims
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IPC IPC(8): C12N1/20A62D3/02B09C1/10C02F3/34C02F103/36C12R1/01A62D101/26A62D101/04
Inventor 赵萍王雅张轶亓文静
Owner LANZHOU UNIVERSITY OF TECHNOLOGY