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Method for genetic transformation of agrobacterium rhizogenes-mediated anthrium andraeanum

A genetic transformation method and Agrobacterium-mediated technology, applied in the field of Agrobacterium-mediated genetic transformation of Anthurium angustifolia

Inactive Publication Date: 2010-09-15
崔婧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there are very few reports on the genetic transformation of Anthurium mediated by Agrobacterium at home and abroad

Method used

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  • Method for genetic transformation of agrobacterium rhizogenes-mediated anthrium andraeanum

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Experimental program
Comparison scheme
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Embodiment Construction

[0008] 1. Materials and methods

[0009] 1. Plant material

[0010] Anthurium callus of four varieties of Alabama, Arizona, Flame, and Fenchampion are preserved and provided by our laboratory.

[0011] 2. Vector and strain

[0012] The plant expression vectors pBIiaaM, pBIipt, and pBIiaaM-ipt constructed in this laboratory, and Agrobacterium LBA4404 are preserved in this laboratory.

[0013] 3. Medium

[0014] (1) YEB (Agrobacterium tumefaciens medium)

[0015] Peptone 5g / L

[0016] Sucrose 5g / L

[0017] Beef Extract 5g / L

[0018] Yeast extract 10g / L

[0019] MgSO4 0.495g / L

[0020] (2) The concentration of macroelements in MS basal medium is changed to 1 / 2, and the content of other substances is the same as that of MS basal medium.

[0021] Table 1 Anthurium callus induction and medium used for transformation (mg / L)

[0022]

[0023] 4. Polymerase Chain Reaction (PCR)

[0024] (1) Reaction system

[0025] DNA template 1μl

[0026] H2O 32μl

[0027] 10×Taq buf...

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Abstract

The invention discloses a method for genetic transformation of agrobacterium rhizogenes-mediated anthrium andraeanum, which belongs to the field of plant genetic engineering. The method comprises the following main steps of: performing co-culturing on a callus, serving as explants, of the anthrium andraeanum and agrobacterium rhizogenes; and transferring the mixture to a selective culture medium to perform screening culture until resistance bugs grow into complete plants. The method performs the genetic transformation on the anthrium andraeanum, and the obtained transformation ratio is 5.5 percent which shows that the method has a high transformation ratio. Therefore, the method lays a foundation for stable expression of an exogenous gene in the anthrium andraeanum, and has a great significance for genetic improvement of the anthrium andraeanum.

Description

technical field [0001] The invention relates to an agrobacterium-mediated genetic transformation method of anthurium. Background technique [0002] Anthuriun andreanum L. is a perennial herb of the genus Anthuriun in the family Araceae. Native to the tropical rainforest of South America, it grows in a humid and semi-shady valley environment, prefers shade and heat, and is currently widely cultivated in the Netherlands, Israel and Hawaii. Anthurium is a high-end flower that is very popular and loved by people in the world. Its flower shape is noble and elegant, its flower branches are unique, and it can bloom all year round. It has become a popular high-end cut flower material and potted plant species in the world. The quality of many ornamental plants is affected by high or low temperature, and anthurium is no exception. It is very sensitive to temperature: the suitable growth temperature is 25°C-28°C; when the temperature exceeds 30°C, it will brown and die; below 18°C, i...

Claims

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Application Information

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IPC IPC(8): C12N15/82
Inventor 崔婧
Owner 崔婧
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