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Method for identifying and evaluating cold resistance of bamboo

A technology of cold resistance and bamboo species, applied in the direction of biochemical equipment and methods, material resistance, preparation of test samples, etc.

Inactive Publication Date: 2010-10-06
胡尚连
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The present invention is aimed at the above technical problems, and provides a method for identification and evaluation of bamboo cold resistance that solves the problem of using seeds to obtain small bamboos with consistent growth for cold resistance identification.

Method used

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  • Method for identifying and evaluating cold resistance of bamboo
  • Method for identifying and evaluating cold resistance of bamboo
  • Method for identifying and evaluating cold resistance of bamboo

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Experimental program
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Embodiment

[0026] The method for identification and evaluation of bamboo cold resistance comprises the following steps:

[0027] a) get the same bamboo age and 30cm branches with consistent growth as material, wash and dry, seal with paraffin, process 12h under low temperature conditions, take untreated branches as contrast;

[0028] b) A part of the treated leaves was quick-frozen in liquid nitrogen for 30 minutes and then stored in a -80°C refrigerator. The other part of the leaves was used for conductivity analysis. Each sample was repeated 3 times to measure the conductivity and solubility of different bamboo species after treatment. Sugar content, soluble protein content, malondialdehyde, proline, superoxide dismutase activity, peroxidase activity;

[0029] c) According to the gray system theory, the gray system is established with the physiological and biochemical indexes and the mean value of the cold resistance membership function of different bamboo species as the data set, and ...

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Abstract

The invention relates to a technology system applicable to identification and evaluation of cold resistance of isolated parts of different bamboo varieties, in particular to a method for identifying and evaluating the cold resistance of bamboos. The method for identifying and evaluating the cold resistance of the bamboos is characterized by comprising the following steps of: a) taking a twig of the same bamboo age and with a length of 30 centimeters as a reference; b) quick-freezing a part of processed leaves in liquid nitrogen for 30 minutes and then storing the leaves into a refrigerator at the temperature of 80 DEG C below zero and the like. The method for identifying and evaluating the cold resistance of the bamboos is characterized by: a) conductivity determination; b) determination of soluble sugar content; c) determination of soluble protein content; d) malondialdehyde (MDA) determination; e) praline determination; f) determination of activities of superoxide dismutase (SOD); and g) determination of activities of peroxidase (POD).

Description

technical field [0001] The invention relates to a technical system suitable for identification and evaluation of cold resistance of different bamboo species in vitro, in particular to a method for identification and evaluation of cold resistance of bamboo. Background technique [0002] In the past, the research on bamboo was mostly focused on cultivation and promotion, and the research on the identification and evaluation technology system of bamboo cold resistance in vitro has not been reported. Because it is difficult to obtain seeds for bamboo, the evaluation of bamboo cold resistance is mainly for the natural conditions. The growth of bamboo, the study of its single physiological and biochemical indicators related to cold resistance, and the individual growth is inconsistent, can not objectively evaluate the cold resistance of bamboo, which has certain limitations. Contents of the invention [0003] The present invention aims at the above technical problems and provide...

Claims

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Application Information

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IPC IPC(8): G01N27/06G01N21/78G01N1/30C12Q1/26C12Q1/28
Inventor 胡尚连陈珂曹颖任鹏卢学琴段宁
Owner 胡尚连
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