Application of ethyl linoleate to prevention and treatment of pine wilt diseases

A technology of ethyl linoleate and pine wood nematode, which is applied in application, nematicides, chemicals for biological control, etc., can solve problems such as ineffective effects, and achieve easy popularization and application, stable structure, and easy operation simple effect

Inactive Publication Date: 2010-10-13
INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control of pine wood nematode disease mainly depends on removing all diseas...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] In the area where B. xylophilus occurs, 20 Pinus massoniana containing reproductive B. xylophilus were selected. The identification method is as follows: the pine tree with pine xylophilus damage does not flow fat after drilling, the side branches have traces of feeding by the pine tree beetle, and some pine needles lose green. 10 strains are used as the experimental group, and 10 strains are used as the control group, and holes with a diameter of 1 cm and a depth of 2 cm are drilled with an electric drill at different heights on the sunny side of the tree body. The dose of ethyl linoleate was directly dropped into the wells of the experimental group at a dose of 1 μl / well, and the dosage of ethyl linoleate was 5 μl / m 3 Pine tree body. In the control group, no ethyl linoleate was added, and after 10 days, the pine wood nematodes isolated from Pine massoniana by the Baermann funnel method were observed under a microscope. The results showed that the experimental group ...

Embodiment 2

[0021] Ethyl linoleate was dissolved in methanol and adsorbed on a rubber septa (0.5 cm diameter, 1 cm length). In the area where B. xylophilus occurs, 20 Pinus massoniana containing reproductive B. xylophilus were selected. The identification method is the same as in Example 1. 10 strains are used as the experimental group, and 10 strains are used as the control group, and holes with a diameter of 1 cm and a depth of 2 cm are drilled with an electric drill at different heights on the sunny side of the tree body. In the experimental group, a number of silicone rubber plugs containing 1 μl of ethyl linoleate were scattered and inserted into the holes (after mixing 5 μl of ethyl linoleate and 100 μl of methanol, take 20 μl and drop them into the silicone rubber plugs, which can be used after the solvent evaporates). The dosage of ethyl linoleate is 5μl / m 3 Pine tree body. In the control group, a silicone rubber plug containing 1 μl of methanol was inserted, and after 10 days, ...

Embodiment 3

[0023] Ethyl linoleate was dissolved in ethanol and adsorbed on a silicone rubber stopper (0.5 cm diameter, 1 cm length). In the area where B. xylophilus occurs, 20 Pinus massoniana containing reproductive B. xylophilus were selected. The identification method is the same as in Example 1. 10 strains are used as the experimental group, and 10 strains are used as the control group, and holes with a diameter of 1 cm and a depth of 2 cm are drilled with an electric drill at different heights on the sunny side of the tree body. In the experimental group, a number of silicone rubber plugs containing 1 μl of ethyl linoleate were scattered and inserted into the holes (after mixing 5 μl of ethyl linoleate and 100 μl of ethanol, take 20 μl and drop them into the silicone rubber plugs, which can be used after the solvent evaporates). The dosage of ethyl linoleate is 5μl / m 3 Pine tree body. In the control group, a silicone rubber plug containing 1 μl of ethanol was inserted, and after 1...

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Abstract

The invention relates to application of ethyl linoleate to prevention and treatment of pine wilt diseases. A method of using the ethyl linoleate for preventing and treating the pine wilt diseases, provided by the method, comprises the step of placing the ethyl linoleate in pines containing propagation type pine wood nematodes. The method provided by the invention can be used for the governing of pines infected with pine wood nematodes in forest. The invention can solve the problems of mass propagation and fast diffusion of the pine wood nematodes in pine wood nematode epidemic situation generating places.

Description

technical field [0001] The invention relates to the application of ethyl linoleate in the prevention and treatment of pine wood nematode disease. Background technique [0002] Pine wood nematode disease, known as the "cancer" of pine trees, is the most dangerous forest disease in the world. It is a devastating disease of pinaceae plants. The entire pine forest can be destroyed within 5 years, which is extremely harmful. Since the first discovery of pine wood nematode disease in Nanjing in 1982 in my country, the epidemic has spread rapidly and spread to 14 provinces and cities including Jiangsu, Anhui, Guangdong, Guangxi, Zhejiang, Fujian, Shanghai, Chongqing, Hubei, Shandong, and 86 counties. . In the past 28 years, more than 500 million pine trees have been killed, more than 5 million mu of pine forests have been destroyed, and hundreds of billions of yuan in economic losses have been caused, and the scope of occurrence is still expanding year by year. It not only causes...

Claims

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Application Information

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IPC IPC(8): A01N37/06A01P5/00
Inventor 赵莉蔺孙江华韦卫
Owner INST OF ZOOLOGY CHINESE ACAD OF SCI
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