Method for inducing sugarcane callus by using Lingfasu

A technology of callus and spirit hair, which is applied in the field of sugarcane callus induced by spirit hair, can solve the problems of residue, toxicity, deficiency, etc., and achieve the effect of non-hardening texture, strong vitality, and loose texture

Inactive Publication Date: 2010-10-20
广西壮族自治区甘蔗研究所
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Problems solved by technology

However, 2,4-D is an artificial chemically synthesized benzene derivative, which is toxic and residual, and the concentration used to induce sugarcane callus is as high as 2.0-3.0mg·L -1 , the induction rate is often less than 50% at low concentrations
Furthermore, the callus induced by 2,4-D is easy to brown and harden, which directly affects the continued proliferation and differentiation of callus, and severe browning even leads to the death of explants.
For this reason, technicians have to frequently transfer explants to fresh medium, which is troublesome and labor-intensive

Method used

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  • Method for inducing sugarcane callus by using Lingfasu

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Embodiment Construction

[0008] The technical solution of the present invention will be further described below through examples.

[0009] The method for inducing sugarcane callus with lingfa essence of the present invention comprises the steps:

[0010] Collect the young leaves of sugarcane terminal parts as explants according to routine, cut into about 1,0×0.5cm after surface disinfection and sterilization 2 The small pieces are respectively connected with 0.05-0.50mg L of Lingfa -1 The modified MS solid medium was used for the induction of sugarcane callus. Culture conditions: pH 5.8-6.0; light 1500-2000lx.10-12 hours / day; temperature: day / night 28 / 25(±1)℃.

[0011] Example 1 in Table 1 is a comparative example, and Examples 2 to 5 are examples of adding different amounts of ling hair essence, and the rest of the operating conditions and method steps are the same.

[0012] Table 1. The comparison experiment data of sugarcane callus induced by adding different amounts of Lingfasu and 2,4-D

[00...

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Abstract

The invention discloses a method for inducing sugarcane callus by using Lingfasu. In the method, an MS is used as a basal culture medium, 0.05 to 0.5 mg.l-1 of Lingfasu is added into the basal culture medium to ensure that the basal culture medium is prepared into an improved type solid culture medium, and a sugarcane spire slice is used as an explant to perform sterile culture by the book. The method is used for inducing the sugarcane callus, the inducing efficiency is up to 90 to 95 percent, and the callus cannot be browned easily, has a loosen texture, is not hardened, is proliferated quickly and has strong vital force.

Description

technical field [0001] The invention relates to a method for inducing sugarcane callus with lingfa essence. technical background [0002] The acquisition of sugarcane callus is considered to be a necessary link for the successful establishment of various modern biotechnology systems such as rapid propagation of sugarcane varieties, detoxification and rejuvenation, genetically modified breeding, and radiation-induced mutation breeding in the sugarcane planting industry. However, the existing available data show that the plant growth substances (Plant growth substances) currently used to induce the callus of sugarcane (mostly the explants are young leaves of sugarcane shoots) belong to the auxin class (Auxin) almost without exception. Including 2,4-D (2,4-dichlorophenoxyacetic acid), NAA (naphthalene acetic acid) and IBA (indole butyric acid), among which 2,4-D is the most commonly used, and its effect is also considered most. However, 2,4-D is an artificial chemically synth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 蓝桃菊许鸿源方锋学李松余坤兴刘红坚刘晓燕谭芳龚银花刘丽敏淡明杨荣仲游建华唐红琴卢曼曼
Owner 广西壮族自治区甘蔗研究所
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