Tissue culture method for pennisetum setaceum 'Rubrum'

A technology of Pennisetum purpurea and tissue culture, which is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of limited supply of seedlings, low number of introductions of Pennisetum purpurea, etc., and achieves improved stability. , the effect of improving reproduction speed and uniformity of seedlings

Inactive Publication Date: 2010-10-27
SHANGHAI SHANGFANG LANDSCAPE PLANT INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a new variety introduced from abroad, the number of introduced Pennisetum purple leaves is relatively small, and the supply of seedlings is subject to certain restrictions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] (1) Obtaining sterile materials

[0029] Take the young buds that have germinated in spring, remove the wrapped leaves, wash them with tap water for 1 hour, put them on the ultra-clean workbench, soak them in ethanol with a mass concentration of 70% for 10 seconds, and then soak them in mercury with a volume concentration of 0.5‰ for 10 minutes. Rinse 4 times with sterile water again, use sterile filter paper to blot the moisture on the surface, then cut into 0.5cm-long segments with axillary buds, and inoculate the segments with MS+6-BA1.0mg / L+NAA0. On the axillary bud induction medium of 1mg / L;

[0030] (2) Differentiation and proliferation of buds

[0031] After the segments were inoculated on the axillary bud induction medium for 1 week, the axillary buds began to expand and green protrusions appeared. After 3 weeks, bud meristems could be seen. After culturing for 1 month, the small adventitious buds could grow to 3 cm. Cut the adventitious buds Under transfer in...

Embodiment 2

[0040] (1) Obtaining sterile materials

[0041] Take the young buds that have germinated in spring, remove the wrapped leaves, wash them with tap water for 2 hours, put them on an ultra-clean workbench, and soak them in ethanol with a mass concentration of 75% for 30 seconds, and mercury with a volume concentration of 1‰ for 15 minutes. Rinse 5 times with sterile water, use sterile filter paper to blot the water on the surface, then cut into 1cm-long segments with axillary buds, and inoculate the segments with MS+6-BA5.0mg / L+NAA0.5mg / L on the axillary bud induction medium;

[0042] (2) Differentiation and proliferation of buds

[0043] After the segments were inoculated on the axillary bud induction medium for 2 weeks, the axillary buds began to expand and green protrusions appeared. After 4 weeks, bud meristems could be seen. After culturing for 1 month, the small adventitious buds could grow to 4cm. Cut the adventitious buds Under transfer into the adventitious bud prolif...

Embodiment 3

[0052] (1) Obtaining sterile materials

[0053]Take the young buds that have germinated in spring, remove the leaves wrapped outside, rinse them with tap water for 3 hours, put them on the ultra-clean workbench, and soak them in ethanol with a mass concentration of 75% for 50 seconds, and mercury with a volume concentration of 2‰ for 30 minutes. Rinse 6 times with sterile water, use sterile filter paper to blot the water on the surface, then cut into 2cm-long segments with axillary buds, and inoculate the segments with MS+6-BA5.0mg / L+NAA0.5mg / L on the axillary bud induction medium;

[0054] (2) Differentiation and proliferation of buds

[0055] After the segments were inoculated on the axillary bud induction medium for 3 weeks, the axillary buds began to expand and green protrusions appeared. After 5 weeks, bud meristems could be seen. After 2 months of culture, the small adventitious buds could grow to 4cm. Cut the adventitious buds Under transfer into the adventitious bud...

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Abstract

The invention relates to a tissue culture method for pennisetum setaceum 'Rubrum'. The method comprises the following steps of: obtaining sterile materials; differentiating and proliferating buds; culturing strong seedlings of adventitious buds; rooting; and hardening off seedlings and transplanting the seedlings and the like. Compared with the prior art, the method greatly improves the reproductive speed of the pennisetum setaceum 'Rubrum' and the trimming of the seedlings, and improves the stability of shape and properties of the pennisetum setaceum 'Rubrum', so that industrial mass production of the seedlings can be realized.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method of Pennisetum purple leaves. Background technique [0002] Pennisetum purple leaf is a plant of the genus Pennisetum in the Gramineae family, distributed in tropical and subtropical regions. It is a one-year or biennial herbaceous plant with a plant height of 80cm. The leaves are gathered at the base, the leaves are flat, purple linear, and the inflorescences are conical. Pennisetum is a famous ornamental grass abroad, which can be used for landscaping or on the shore. The spikes of Pennisetum leaves are purple, and the garden cultivation is spectacular and elegant. It can also be used in flower borders and ornamental grass gardens It has excellent ecological adaptability and ornamental value, and is a good flower mirror and ground cover material. However, as a new variety introduced from abroad, the number of introduced Pennisetum purple leaves is relativ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈建华黄建荣沈勤
Owner SHANGHAI SHANGFANG LANDSCAPE PLANT INST
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