Method for constructing Tibetan antelope skin fibroblast line

A technology of fibroblasts and construction methods, applied in the field of construction of Tibetan antelope skin fibroblast cell lines, to achieve the effect of improving survival rate and avoiding damage

Inactive Publication Date: 2010-10-27
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Abstract
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Problems solved by technology

Among the many media, which medium can cultivate Tibetan antelope skin fibroblasts and the specific method of establishing Tibetan antelope skin fibroblast cell lines has not been reported in detail

Method used

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  • Method for constructing Tibetan antelope skin fibroblast line
  • Method for constructing Tibetan antelope skin fibroblast line

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Experimental program
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Embodiment Construction

[0026] Materials: The ear skin tissue donors used in this experiment were female adult Tibetan antelopes with severe cerebral echinococcosis. The Tibetan antelope is an artificially domesticated Tibetan antelope approved by the State Forestry Administration of our research group.

[0027] Reagent:

[0028] Duchenne's phosphate buffer solution (D-PBS) is composed of 0.8g / 100mL NaCl, 0.0203g / 100mL KCl, 0.1152g / 100mL NaCl 2 HPO 4 , 0.0203g / 100mL of KH 2 PO 4 , 0.0134g / 100mL of CaCl 2 2H 2 O, 0.01g / 100mL of MgCl 2 ·6H 2 O, 0.0036g / 100mL of sodium pyruvate, 0.0075g / 100mL of penicillin, 0.005g / 100mL of streptomycin and 0.1g / 100mL of glucose.

[0029] The stock solution is T20 with double antibody added; said T20 with double antibody is cultured on the basis of 20% fetal bovine serum (FBS) and 80% TCM199 containing 10mM hydroxyethylpiperazineethanesulfonic acid in total solution volume Solution composition, and after the configuration, add the double antibody, and then use N...

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Abstract

The invention relates to a method for constructing a Tibetan antelope skin fibroblast line, which comprises the following steps of: (1) sampling; (2) carrying out primary culture of Tibetan antelope ear skin tissue; (3) carrying out passage and purification; (4) carrying out cell cryopreservation; and (5) carrying out cell recovery. By establishing the method for primary culture, cell passage, cell cryopreservation and cell recovery, the invention obtains a high-activity Tibetan antelope fibroblast line, and provides research materials and technical support for further developing related researches of Tibetan antelope molecular biology and cell biology.

Description

technical field [0001] The invention relates to a fibroblast culture, in particular to a method for constructing a Tibetan antelope skin fibroblast cell line. Background technique [0002] Tibetan antelope (Pantholops hodgsoni) belongs to the genus Tibetan antelope of the Artiodactyla Bovidae goat subfamily, a unique species on the Qinghai-Tibet Plateau, a national first-class protected animal and a trade activity prohibited by the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Endangered animals are mainly distributed in the alpine desertification grasslands, alpine grasslands and alpine meadows of Qinghai, Tibet, and Xinjiang provinces in China, and live in harsh environments between 4100 and 5500m above sea level on average. Due to environmental factors, it is very difficult to study Tibetan antelope in depth. At present, the research on Tibetan antelope is mainly based on macrozoological research, including: animal behavior, popul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12R1/91
Inventor 赵新全于鸿浩郭志林郭松长曹俊虎徐世晓
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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