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Method of producing cellulase and cellooligosaccharide

A manufacturing method and cellulase technology, which are applied in the field of high-yield production of cellooligosaccharides and selectivity, can solve problems such as complicated procedures, and achieve the effects of high selectivity and high cellulase activity.

Inactive Publication Date: 2010-11-10
ASAHI KASEI KK
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is a problem that the process is very complicated because the solid part must be subjected to the cellulase adsorption step and the solid part separation step.

Method used

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  • Method of producing cellulase and cellooligosaccharide
  • Method of producing cellulase and cellooligosaccharide
  • Method of producing cellulase and cellooligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Trichoderma reesei GL-1 strain (Institute of Advanced Industrial Science and Technology, Patent Organism Depository Center, deposit number: FERM BP-10323) was cultured on a potato dextrose agar slant medium at 28°C for 7 days to fully form spore. Cottonseed meal of 1 Platinum ring (manufactured by culture medium, Treader Corporation): 0.15 g, KH 2 PO 4 : 0.06g, (NH 4 ) 2 SO 4 : 0.045g, MgSO 4 ·7H 2 O: 0.009g, CaCl 2 2H 2 O: 0.009g, Adekanol (registered trademark) LG109: 0.03mL, trace element solution (H 3 BO 3 6mg (NH 4 ) 6 Mo 7 o 24 4H 2 O 26mg FeCl 3 ·6H 2 O 100mgCuSO 4 ·5H 2 O 40mg MnSO 4 ·5H 2 O 8mg ZnSO 4 ·7H 2 (200mg dissolved in 100ml of water and suspended solution): 0.03ml dissolved in water: 30ml and suspended, adjusted to pH 4.0, dispensed 30ml into a Erlenmeyer flask with a capacity of 150ml, added crystalline cellulose (manufactured by Asahi Kasei Chemicals, commercial product) Namely PH-101) 0.3 g, inoculated into the medium for prec...

Embodiment 2

[0078] The Trichoderma reesei GL-1 strain was cultured on a potato dextrose agar slant medium at 28°C for 7 days to fully form spores. 1 Platinum ring and cottonseed meal (medium, manufactured by Toleda Isu): 0.15g, KH 2 PO 4 : 0.06g, (NH 4 ) 2 SO 4 : 0.045g, MgSO 4 ·7H 2 O: 0.009g, CaCl 2 2H 2O: 0.009g, Adekanol (registered trademark) LG109: 0.03mL, trace element solution (H 3 BO 3 6mg (NH 4 ) 6 Mo 7 o 24 4H 2 O 26mgFeCl 3 ·6H 2 O 100mg CuSO 4 ·5H 2 O 40mg MnSO 4 ·5H 2 O 8mg ZnSO 4 ·7H 2 (200mg dissolved in 100ml of water and suspended solution): 0.03ml dissolved in water: 30ml and suspended, adjusted to pH 4.0, dispensed 30ml into a Erlenmeyer flask with a capacity of 150ml, added crystalline cellulose (manufactured by Asahi Kasei Chemicals, commercial product) Namely PH-101) 0.3 g, inoculated into the medium for preculture sterilized by an autoclave, and precultured at 28° C. for 3 days.

[0079] Next, the culture solution was mixed with cottonseed me...

Embodiment 3

[0083] The Trichoderma reesei GL-1 strain was cultured on a potato dextrose agar slant medium at 28°C for 7 days to fully form spores. 1 platinum ring and cottonseed meal (manufactured by culture medium, Treida Isu Co.): 0.15 g, KH 2 PO 4 : 0.06g, (NH 4 ) 2 SO 4 : 0.045g, MgSO 4 ·7H 2 O: 0.009g, CaCl 2 2H 2 O: 0.009g, Adekanol (registered trademark) LG109: 0.03mL, trace element solution (H 3 BO 3 6mg (NH 4 ) 6 Mo 7 o 24 4H 2 O 26mgFeCl 3 ·6H 2 O 100mg CuSO 4 ·5H 2 O 40mg MnSO 4 ·5H 2 O 8mg ZnSO 4 ·7H 2 (200mg dissolved in 100ml of water and suspended solution): 0.03ml dissolved in water: 30ml and suspended, adjusted to pH 4.0, dispensed 30ml into a Erlenmeyer flask with a capacity of 150ml, added crystalline cellulose (manufactured by Asahi Kasei Chemicals, commercial product) Namely PH-101) 0.3 g, inoculated into the medium for preculture sterilized by an autoclave, and precultured at 28° C. for 3 days.

[0084] Then, the culture solution was mixed with...

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Abstract

It is intended to provide a method of producing an enzyme solution whereby a culture supernatant enzyme solution having a high cellulase activity and showing a high cellooligosaccharide selectivity can be surely obtained in a large amount in selectively producing cellobiose at a high yield by enzymatically digesting a cellulose-based starting material in the presence of cellulase; and a method ofproducing a cellooligosaccharide with the use of the enzyme solution as described above. Namely, a method of producing an enzyme solution containing cellulase which comprises culturing a microorganism of Trichoderma genus in a liquid medium containing a component originating in seeds of a plant of Malavaceaefamily.

Description

technical field [0001] The present invention relates to a method for selectively and high-yield production of cellooligosaccharides, a method for producing an enzyme solution having high cellulase activity and a high selectivity for cellooligosaccharides, and a method for producing cellooligosaccharides using the enzyme solution Production method. Background technique [0002] Cellooligosaccharides are a general term for cellobiose, cellotriose, cellotetraose, cellopentaose, and cellohexaose, and are various sugars in which 1 to 6 glucopyranose units are bonded by β-1,4. In recent years, like other oligosaccharides, the physiological functions of cellooligosaccharides have become increasingly clear, and they are expected to be used as new materials for functional foods (see Non-Patent Document 1). Generally, known methods for obtaining cellobiose include (1) a method using an enzymatic synthesis reaction using sucrose as a raw material and (2) a method using an enzymatic de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12P19/14
CPCC12R1/885C12P19/14C12N9/2437C12N1/145C12R2001/885
Inventor 小西一诚井阪光二山崎有亮
Owner ASAHI KASEI KK