Method for extracting white mushroom lentinan assisted with ultrasonic wave

The technology of Agaricus bisporus and ultrasonic wave is applied in the direction of antitoxin, antibacterial drug, drug combination, etc., which can solve the problems of easily destroyed polysaccharide, complex process, many extraction steps, etc., and achieves a simple process of overcoming the limitation of production season and simple process. Effect

Inactive Publication Date: 2010-11-24
乔德亮
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the problems existing in the prior art that strong acid and strong alkali operations easily destroy the structure of polysaccharides, there are many extraction steps, the process is complicated and the purity of polysaccharides is not high; the purpose of th

Method used

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  • Method for extracting white mushroom lentinan assisted with ultrasonic wave
  • Method for extracting white mushroom lentinan assisted with ultrasonic wave
  • Method for extracting white mushroom lentinan assisted with ultrasonic wave

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A method for ultrasonically assisted extraction of Agaricus bisporus polysaccharides includes the following steps:

[0023] (1) Cut the fresh fruit bodies of Agaricus bisporus into small pieces, dry at 60°C, and crush to obtain crushed material;

[0024] (2) According to the water-to-material ratio of 30mL / g, add deionized water to the crushed material of step (1) and place it in an ultrasonic cleaner for hot water extraction. The extraction temperature is 65℃, the time is 40min, and the ultrasonic power is 170w. liquid;

[0025] (3) Centrifuge the extract of step (2), take the supernatant, and centrifuge the conditions: 5000rpm, 15min; use the same method to extract the precipitate, centrifuge, take the supernatant, and combine the 2 supernatants ;

[0026] (4). Concentrate the supernatant of step (3) at a temperature of 50°C under reduced pressure to 1 / 10 of the original volume to obtain a concentrated solution; add 3 times the volume of absolute ethanol to the concentrated ...

Embodiment 2

[0037] Optimization experiment of extraction conditions for the ultrasonic-assisted extraction of Agaricus bisporus polysaccharide

[0038] (1) Ultrasonic-assisted extraction of Agaricus bisporus polysaccharides extraction conditions optimization of single factor experiment

[0039] ①The effect of extraction temperature on the extraction rate of Agaricus bisporus polysaccharide.

[0040] The extraction temperature was set to five gradients of 20, 35, 50, 65, and 80°C, and the extraction time, ultrasonic power, and water-to-material ratio were 40 min, 170 w, and 40 mL / g, respectively. The extraction rate of polysaccharides at different extraction temperatures is shown in Table 1 below:

[0041]

[0042] It can be seen from Table 1 that the extraction rate of polysaccharides is the highest at 50°C, so 50°C is chosen as the center point of the following orthogonal experiment.

[0043] ②The effect of extraction time on the extraction rate of Agaricus bisporus polysaccharide.

[0044] Five g...

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Abstract

The invention relates to a method for extracting white mushroom lentinan assisted with ultrasonic wave, which comprises the following steps: drying and crushing the white mushroom sporocarp; extracting with hot water assisted by ultrasonic wave; taking supernate by centrifugation; concentrating under the reduced pressure; precipitating with ethanol; taking precipitate by centrifugation; removing protein by a Sevag method; concentrating under the reduced pressure; precipitating with ethanol; taking precipitate by centrifugation; removing pigment by a macroporous resin method; concentrating under the reduced pressure; and vacuum drying to obtain the extract of the white mushroom lentinan. The invention has mild extraction condition, does not damage the lentinan structure, and has high polysaccharose purity, simple technical process, no environment pollution and low energy consumption. Additionally, the invention takes the dried and crushed white mushroom sporocarp as raw materials for extracting and overcomes the defect of season limitation caused by the utilization of the fresh raw materials.

Description

Technical field [0001] The invention belongs to the technical field of extracting relevant components from the genus Agaricus, and specifically relates to a method for extracting polysaccharides from Agaricus bisporus. Background technique [0002] Agaricus bisporus, whose Chinese aliases are mushrooms, western mushrooms, white mushrooms, etc., are often called common cultivated mushrooms or button mushrooms in European and American countries. Agaricus bisporus is taxonomically classified as Eumycota, Basidiomycotina, Hymenomycetes, Agaricales, Agaricaceae ), mushrooms (Agaricus). Chinese medicine believes that Agaricus bisporus is cool, sweet and non-toxic. Mushroom enters the three meridians of the intestines, stomach and lungs, and has the effects of appetizing, regulating qi, eliminating phlegm, euphoria, detoxification, rash, antiemetic, antidiarrheal, digestion, refreshing, and calming liver-yang. Indications of fever in the middle and late stages of body fatigue, qi def...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P31/04A61P35/00A61P37/04A61P39/06
Inventor 乔德亮陈乃富曾晓雄王守红韦传宝张莉
Owner 乔德亮
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