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Protein related to synthesis of mono-vinyl chlorophyll a and coding gene thereof

A technology of chlorophyll and monoethylene, applied in genetic engineering, plant genetic improvement, cells modified by introducing foreign genetic material, etc., can solve the problem of not cloning diethylene reductase

Inactive Publication Date: 2012-01-04
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

To date, no disvinyl reductase (i.e., C-8 vinyl reductase) gene has been cloned in monocots, including rice

Method used

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  • Protein related to synthesis of mono-vinyl chlorophyll a and coding gene thereof
  • Protein related to synthesis of mono-vinyl chlorophyll a and coding gene thereof
  • Protein related to synthesis of mono-vinyl chlorophyll a and coding gene thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, the cloning and verification of the rice diethylene chlorophyll a reductase gene

[0031] 1. Cloning of Rice Disethylene Chlorophyll-a Reductase Gene

[0032] 1. Gene discovery

[0033] The present inventors isolated a spontaneous mutant 824ys ( figure 1 ), the mutant showed yellow-green leaf type, decreased chlorophyll content, inhibited chloroplast development, slow plant growth, significantly reduced tillering, etc. (Huang Xiaoqun, Wang Pingrong, Zhao Haixin, Deng Xiaojian. Inheritance of a new rice chlorophyll-deficient mutant gene Analysis and molecular marker mapping. China Rice Science, 2007, 21(4): 355-359). Genetic analysis revealed that the mutant was controlled by a pair of recessive nuclear genes. A gene Os03g22780 homologous to Arabidopsis DV Chlide a reductase gene (DVR) was identified by map-based cloning method and named OsDVR. OsDVR is located on the short arm of rice chromosome 3, its nucleotide sequence is shown in Sequence Listing S...

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Abstract

The invention discloses a protein related to synthesis of mono-vinyl chlorophyll a and a coding gene thereof. The protein related to the synthesis of the mono-vinyl chlorophyll a is a protein as the following 1) or 2): 1) a protein composed of an amino acid sequence shown in a sequence 2 of a sequence table; and 2) a protein derived from 1), related to the synthesis of mono-vinyl chlorophyll a, and obtained by substituting and / or deleting and / or adding one or more amino acid residues in an amino acid residue sequence of the sequence 2 in the sequence table. Experiments prove that the protein provided by the invention can convert divinyl chlorophyll a into the mono-vinyl chlorophyll a.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a protein related to the synthesis of monoethylene chlorophyll a and its coding gene. Background technique [0002] Chlorophyll is an important pigment for photosynthesis in plants. Its function is to capture light energy and transfer light energy to the reaction center, which plays an important role in the growth and yield of plants. According to the number of ethylene side chains, chlorophyll can be divided into 3,8-divinyl chlorophyll (DV Chls) and 3-vinyl chlorophyll (monoethylene chlorophyll, MV Chls). Under normal circumstances, the chlorophyll used by almost all plants and bacteria for photosynthesis is MV chls, and only one type of marine organisms, namely "the marine prochlorophyte Prochlorococcus marinus", has been found to use DV Chls (Chisholm SW, Frankel SL, Goericke R, Olson RJ, Palenik B, Waterbury JB, West-Johnsrud L, Zettler ER (1992) Prochlorococcus marinus nov.gen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/63C12N1/19C12N15/11C12N1/21C12N9/02C12N5/10C12N15/53C12P17/18C12N1/15
Inventor 王平荣邓晓建高家旭万春美张帆涛
Owner SICHUAN AGRI UNIV