Container and method for detecting nucleic acid reaction

A container and nucleic acid technology, which is applied in specific-purpose bioreactors/fermenters, biochemical equipment and methods, bioreactor/fermenter combinations, etc., can solve the problems of high price, complicated preparation, and poor environmental protection. Achieve low cost, simple operation and avoid false positives

Inactive Publication Date: 2010-12-08
GENEFIRST TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this device is that the preparation of the device is complicated, the price is relatively expensive, and the environmental protection is poor when it is discarded. In this method, although the test tube cover is not opened, the bottom still needs to be pierced, and there is still a risk of leakage.

Method used

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  • Container and method for detecting nucleic acid reaction
  • Container and method for detecting nucleic acid reaction
  • Container and method for detecting nucleic acid reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Such as figure 1 , figure 2 As shown, the container used for nucleic acid reaction detection provided by the present invention is a test tube with a cover. The container can be sealed after the cover 1 is covered. a and compartment b, the upper part of the spacer 3 has a through hole 4, which is the liquid flow channel, and the liquid in different compartments can only flow between the two compartments through the through hole.

[0033] The steps of using the test tube to detect nucleic acid PCR amplification reaction are as follows:

[0034] 1) Add nucleic acid reaction reagents and DNA to compartment a, and nucleic acid fluorescent dye SYBR GREEN or oligonucleotide probe (labeled or unlabeled) to compartment b;

[0035] 2) Cover the cover;

[0036] 3) Carry out PCR amplification reaction;

[0037] 4) After the reaction is completed, tilt the test tube so that the amplification product in compartment a is mixed with the probe or dye in compartment b through the th...

Embodiment 2

[0040] Such as image 3 As shown, the container for nucleic acid reaction detection provided by the present invention is a test tube with a cover. The container can be sealed after the cover is covered. and compartment b, there is a certain distance between the top of the indented gap and the test tube mouth, the indented gap is the septum, and the distance between the top of the indented gap and the test tube mouth is the liquid flow channel, the different compartments Liquid can only flow between the two compartments through this channel.

[0041] The steps of using the test tube to detect the nucleic acid PCR amplification reaction are the same as in Example 1.

Embodiment 3

[0043] Such as Figure 4 As shown, the container used for nucleic acid reaction detection provided by the present invention is a test tube with a cover. The container can be sealed after the cover is covered. The inside of the container body is divided into three different compartments by two partitions. , sub-chamber b and sub-chamber c, the upper part of the diaphragm has a through hole, the through hole is the liquid flow channel, the liquid in different sub-chambers can only flow between the two sub-chambers through the through hole.

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Abstract

The invention provides a container for detecting nucleic acid reaction, which has the advantages of simple and convenient operation and low cost, and a method for detecting the nucleic acid reaction by using the container. The container for detecting the nucleic acid reaction is integrally sealable, and is divided into different chambers by spacers; a liquid flowing channel is formed above the spacers; and liquid in the different chambers can flow among the chamber only through the fluid flowing channel. The container has the advantages of totally enclosed detection, prevention of cross contamination in the detection of the nucleic acid reaction, false negative prevention, simple operation and low cost. The container and the method can be widely used for infectious disease monitoring, human genetic disease monitoring, antenatal and premarital checking, medicolegal expertise or blood relationship identification, husbandry and food industry, environment monitoring and the like.

Description

technical field [0001] The invention relates to a container for nucleic acid reaction detection, especially nucleic acid rapid amplification reaction and amplification product detection, and a method for nucleic acid reaction detection using the container. Background technique [0002] Nucleic acid amplification technology is widely used in medicine, forensic science, molecular biology, gene diagnosis, forensic science and other fields. Due to the strong amplification efficiency, cross-contamination is a challenge for various laboratories. A variety of approaches have been proposed to address this challenge, including the use of UNG / dUTP anti-contamination systems, separate rooms for sample handling, amplification, and detection. [0003] Fluorescent quantitative PCR is a new technology developed in recent years. Since the PCR reaction and detection are carried out in one test tube, the test tube does not need to be opened, which overcomes the shortcoming of the traditiona...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/34C12Q1/68
Inventor 杭建英
Owner GENEFIRST TECH
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