Comprehensive method for extracting L-tryptophan from fermentation liquor

A comprehensive method, tryptophan technology, applied in the field of extracting L-tryptophan, can solve the problems of waste liquid not being reused comprehensively, polluting the environment, wasting, etc., and achieve the effects of purifying the environment, reducing production costs, and reducing waste of resources

Active Publication Date: 2010-12-15
SHANDONG YANGCHENG BIOLOGY TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction of refined L-tryptophan from L-tryptophan fermentation broth is generally carried out in three processes: crude, refined and separated. Each extraction process will produce waste liquid, which contains pigments, impurities, L- Tryptophan, a large amount of N, P, K and organic components, direct discharge will cause waste and pollute the environment
At present, the waste liquid after extraction is generally not scientifically and reasonably comprehensively utilized again, which is a drawback of the current fermentation method to produce L-tryptophan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041]①Put the L-tryptophan fermentation liquid (L-tryptophan content 38.6g / L) into the extraction storage tank, heat to 70°C, adjust the pH value to 4 with industrial sulfuric acid, sterilize through microfiltration membrane, ultra- The filtrate is obtained by removing impurities and decolorizing the filter membrane. The microfiltration membrane used is a ceramic membrane with a pore size of 0.22um. The temperature of the microfiltration is controlled at 70°C, the pressure is 0.25Mpa, and the flow rate is 90 L / m 2 .h; the ultrafiltration membrane used is a roll-type membrane with a molecular weight cut-off of 100,000 D. The ultrafiltration temperature is controlled at 50°C, the pressure is 0.30Mpa, and the flow rate is 80L / m 2 .h;

[0042] ②The filtrate enters the ion exchange column, and the column is filled with 001×7 strong acidic cation exchange resin, which is adsorbed at 30°C with a flow rate of 1.8v / v min, and then eluted with 2.5mol / L ammonia water after adsorption. ...

Embodiment 2

[0053] ①Put the L-tryptophan fermentation broth (L-tryptophan content 39.2g / L) into the extraction storage tank, heat to 60°C, adjust the pH value to 2 with industrial hydrochloric acid, sterilize through microfiltration membrane, ultra- The filtrate is obtained by removing impurities and decolorizing the filter membrane. The microfiltration membrane used is a zirconia membrane with a pore size of 0.15-0.2um. The temperature of the microfiltration is controlled at 60°C, the pressure is 0.35Mpa, and the flow rate is 150 L / m 2 .h; the ultrafiltration membrane used is a hollow fiber membrane with a molecular weight cut-off of 80,000 D. The ultrafiltration temperature is controlled at 60°C, the pressure is 0.20Mpa, and the flow rate is 50L / m 2 .h;

[0054] ②The filtrate enters the ion exchange column, which is filled with sulfonic acid type strong acid ion exchange resin, and is adsorbed at 25°C with a flow rate of 1.0v / v·min. After adsorption, it is eluted with 2.0mol / L ammonia w...

Embodiment 3

[0065] ①Put the L-tryptophan fermentation broth (L-tryptophan content 39.5g / L) into the extraction storage tank, heat to 65°C, adjust the pH value to 3 with industrial hydrochloric acid, sterilize through microfiltration membrane, ultra- The filtrate is obtained by removing impurities and decolorizing the filter membrane. The microfiltration membrane used is an alumina membrane with a pore size of 0.3-0.35um. The temperature of the microfiltration is controlled at 60°C, the pressure is 0.2Mpa, and the flow rate is 60 L / m 2 .h; The ultrafiltration membrane used is a hollow fiber membrane with a molecular weight cut-off of 100,000 D. The ultrafiltration temperature is controlled at 40°C, the pressure is 0.35Mpa, and the flow rate is 100L / m 2 .h;

[0066] ②The filtrate enters the ion exchange column, which is filled with sulfonic acid type strong acid ion exchange resin, and is adsorbed at 35°C with a flow rate of 2.5v / V min, and washed with 0.5mol / L sodium hydroxide solution aft...

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Abstract

The invention discloses a comprehensive method for extracting L-tryptophan from fermentation liquor. The L-tryptophan is extracted from fermentation liquor which is made from corynebacterium glutmicum as a strain; meanwhile, generated waste liquor is recycled. The comprehensive method comprises the steps of: extracting the L-tryptophan and recycling the waste liquor, wherein the waste liquor generated in an extraction process is treated or recycled for application or used for preparing a compound fertilizer, a feed additive and feed protein. The method completely, effectively and comprehensively utilizes resources, recycles the generated waste liquor while extracting the L-tryptophan, reduces the resource waste, basically realizes the zero emission of waste water, effectively recycles the resources, and lightens the environment-protecting pressure.

Description

technical field [0001] The invention relates to a method for extracting L-tryptophan from a fermentation broth, in particular to a method for extracting L-tryptophan from a fermentation broth obtained by fermentation of Corynebacterium glutamicum and treating the waste liquid produced during the extraction process. A comprehensive approach to recycling. Background technique [0002] L-tryptophan is an important amino acid in food and pharmaceutical products. It has a wide range of uses. At present, the production of L-tryptophan by fermentation is an advanced production process. The extraction of refined L-tryptophan from L-tryptophan fermentation broth is generally carried out in three processes: crude, refined and separated. Each extraction process will produce waste liquid, which contains pigments, impurities, L- Direct discharge of tryptophan, a large amount of N, P, K and organic components will cause waste and pollute the environment. At present, the waste liquid aft...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D209/20A23K1/16A23K1/00C05F5/00A23K10/00A23K20/147
CPCY02A40/20
Inventor 王东阳蔡传康闫汝东冯志彬张华
Owner SHANDONG YANGCHENG BIOLOGY TECH CO LTD
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