Enhanced CpGV virus preparation and preparation method thereof

Abstract

The invention discloses an enhanced CpGV virus preparation, which is prepared from the following components by volume percent: 6-30% of activated protein M2R solution, 0.5-1.5% of CpGV suspension, 1-3% of Tween-80 emulsifier and the balance of 1*PBS buffer solution, wherein the sum of volume percent of the components is 100%. The preparation method for the enhanced CpGV virus preparation comprises the following steps: building expressed toxin protein gene engineering strain; inducing expressed toxin protein; collecting the expressed toxin protein; preparing an activity protein M2R solution; and mixing the components to obtain the enhanced CpGV virus preparation of the invention. The preparation method of the enhanced CpGV virus preparation has simple preparation process; and the prepared virus preparation has the advantages of high toxicity and short lethal time, and can obviously improve the control efficiency of the CpGV virus to carpocapsa pomonella.

Description

technical field [0001] The invention belongs to the technical field of pest biological control, and in particular relates to an enhanced CpGV virus preparation, and also relates to a preparation method of the preparation. Background technique [0002] Since the codling moth invaded my country in the middle of the last century, it has seriously occurred in most areas of Xinjiang and Jiuquan, Zhangye, Jiayuguan, Jinchang, Wuwei and other areas in Gansu, and is rapidly moving to Shaanxi and other places, which are the dominant apple producing areas in my country, along the Hexi Corridor. Pushing forward, the situation is very grim. The Wuwei epidemic area in Gansu Province is less than 300 kilometers away from Shaanxi Province, one of our main apple producing areas. If the codling moth is introduced into Shaanxi, it is very easy because there is no extreme geographical isolation zone between Shaanxi and Shandong provinces. Then it quickly spread to the main production area of ​...

AI Technical Summary

Problems solved by technology

The Wuwei epidemic area in Gansu Province is less than 300 kilometers away from Shaanxi Province, one of our main apple producing areas. If the codling moth is introduced into Shaanxi, it is very easy because there is no extreme geographical isolation zone between Shaanxi and Shandong provinces. Then it quickly spread to the main production area of ​​Shandong, which will definitely cause a devastating loss to our apple production

[0003] Codling moth granular virus (CpGV) is one of the main means to control codling moth in Europe, but the control of codling moth in China still mainly relies on chemical pesticides. One of the reasons is that codling moth is a quarantine object in There is no distribution in the main production areas of apples, and it is currently limited to economically underdeveloped areas such as Xinjiang and Gansu. The cost of using virus agents to control pests is high; and the more important reason is that codling moths are mainly distributed in Xinjiang, Gansu and other arid areas. 1. In areas with strong sunlight, insect viruses are used for field control. Viruses are easily inactivated in strong sunlight and high temperature environments, while codling moths are insect species with very serious generation overlap. There are 3-4 generations every year, and long-term and continuous application is required. In order to achieve the control effect, the use of simple virus preparations for prevention and control has a low virus infection rate, a large amount of virus, and high costs; and if chemical pesticides are used in combination, it will not be possible to realize the proliferation of the virus in the pest after the pest is infected with the virus. Killed by pesticides, leading to the loss of the effect of using viruses for biological control, especially the inability to produce the post-effect of persistent epidemics