Immune-enhanced hepatitis B therapeutic multivalent vaccine and preparation method thereof

An immune-enhancing, multivalent vaccine technology, applied in the field of multivalent vaccines, can solve the problems of weak immunogenicity, short half-life, and difficult APC uptake, and achieve the effects of inhibiting replication, increasing activity, and improving development and application prospects.

Inactive Publication Date: 2010-12-22
ARMY MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, simple polypeptide vaccines have inherent defects such as weak immunogenicity, short half-life, and difficult uptake by APCs, which must be modified and improved.

Method used

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  • Immune-enhanced hepatitis B therapeutic multivalent vaccine and preparation method thereof
  • Immune-enhanced hepatitis B therapeutic multivalent vaccine and preparation method thereof
  • Immune-enhanced hepatitis B therapeutic multivalent vaccine and preparation method thereof

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Embodiment Construction

[0029] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0030] 1. Preparation of immunoenhanced hepatitis B therapeutic multivalent vaccine

[0031] 1. Design and synthesis of HBcAg epitope peptide, HBsAg epitope peptide and HBeAg epitope peptide

[0032] The primary structures of HBcAg epitope peptide, HBsAg epitope peptide and HBeAg epitope peptide designed by the present invention are as follows: figure 1 As shown, each epitope is connected with the transmembrane sequence or the endoplasmic reticulum retention signal sequence with a linker sequence,...

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Abstract

The invention discloses an immune-enhanced hepatitis B therapeutic multivalent vaccine and a preparation method thereof. The vaccine is a compound of an HBcAg epitope peptide, an HBsAg epitope peptide, an HBeAg epitope peptide and an LIGHT gene recombinant expression vector, wherein the HBcAg epitope peptide, the HBsAg epitope peptide and the HBeAg epitope peptide respectively comprise epitopes HBcAg87-95/HBsAg172-180/HBeAg147-155, a transmembrane sequence of HIV-Tat49-57, an endoplasmic reticulum retention signal sequence KDEL and a connector sequence, the transmembrane sequence is arranged at the amino terminal, the endoplasmic reticulum retention signal sequence is arranged at the carboxyl terminal, the epitopes are connected with the transmembrane sequence or the endoplasmic reticulum retention signal sequence through the connector sequence, and the vaccine can successfully enter cells and target the endoplasmic reticulum and effectively stimulate hepatitis B virus protein epitope to enter an MHC-I antigen presentation pathway to activate CTL (Cytotoxic Lymphocyte) responses.

Description

technical field [0001] The invention relates to a multivalent vaccine, in particular to an immunoenhancing hepatitis B therapeutic multivalent vaccine, and also relates to a preparation method of the vaccine. Background technique [0002] Hepatitis B virus (HBV) is a hepatotropic DNA virus. Viral hepatitis B, referred to as hepatitis B, is caused by HBV, mainly through blood, body fluids and mother-to-child transmission, and is an infectious disease with a chronic carrier state. At present, there are more than 300 million chronic HBV infections in the world, about half of which are in my country. This kind of infection is easy to develop into chronic hepatitis and cirrhosis, and a small number of cases can be transformed into primary hepatocellular carcinoma. It is currently the ninth cause of death among human diseases announced by WHO. At present, chronic HBV infection is mainly treated with antiviral drugs such as α-interferon and lamivudine, but the curative effect is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K48/00A61K39/295A61K39/29A61P31/20
Inventor 吴玉章杨曌
Owner ARMY MEDICAL UNIV
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