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Fermentation process for improving yield of lucid ganoderma laccase

A fermentation method and technology of Ganoderma lucidum, applied in the field of preparation of industrial enzyme preparations, to achieve the effects of wide source of strains, good induction effect, and good industrial application prospects

Inactive Publication Date: 2012-09-19
INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of fungal elicitors in the liquid fermentation culture of Ganoderma lucidum to increase the production of laccase has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Strain: the fungal elicitor strain is Acremonium fungus Cephalosporium acremonium ( Acremonium strictum ), purchased from the China Microbiological Culture Collection Management Committee, General Microbiology Center, the strain number is CGMCC No. 3.2058.

[0029] Ganoderma lucidum strain is Taishan Red Ganoderma lucidum ( Ganoderma lucidum ), purchased from China Microbiological Culture Collection Management Committee, General Microbiology Center, the strain number is CGMCC No. 5.644.

[0030] A fermentation method for improving the yield of Ganoderma lucidum laccase, the specific steps are:

[0031] (1) Preparation of fungal elicitor

[0032] Inoculate the fungal inducer strains in PD liquid medium (for the cultivation and preservation of filamentous fungi. Peeled potatoes 200g, glucose 20g, boil the potatoes for 20-30 minutes after cutting into pieces, filter and collect the supernatant solution, add glucose, Add water to make up the volume to 1L, sterilize at 1...

Embodiment 2

[0042] Bacterial classification: with embodiment 1.

[0043] A fermentation method for improving the yield of Ganoderma lucidum laccase, the specific steps are:

[0044] (1) Preparation of fungal elicitor

[0045] Inoculate the fungal elicitor strains into PD liquid medium with an inoculum size of 5%, culture them in a shaker at 25°C and 150r / min for 7 days, filter to obtain mycelia, and prepare them by acid hydrolysis (same as Example 1). fungal elicitor.

[0046] (2) Preparation of Ganoderma lucidum seed solution

[0047] The obtained ganoderma lucidum fermentation liquid is filtered several times, and the supernatant liquid is taken to obtain the crude laccase enzyme liquid. Ganoderma lucidum strains were inoculated into PD liquid fermentation medium for fermentation culture, the inoculation amount was 3%, the shaker speed was 150 r / min, the temperature was 25°C, and the fermentation time was 8 days.

[0048] (3) Induction culture of Ganoderma lucidum cells by fungal el...

Embodiment 3

[0055] Bacterial classification: with embodiment 1.

[0056] A fermentation method for improving the yield of Ganoderma lucidum laccase, the specific steps are:

[0057] (1) Preparation of fungal elicitor

[0058] Inoculate the fungal elicitor strains into PD liquid medium with an inoculum size of 5%, culture them in a shaker at 25°C and 150r / min for 7 days, filter to obtain mycelia, and prepare them by acid hydrolysis (same as Example 1). fungal elicitor.

[0059] (2) Preparation of Ganoderma lucidum seed solution

[0060] The obtained ganoderma lucidum fermentation liquid is filtered several times, and the supernatant liquid is taken to obtain the crude laccase enzyme liquid. Ganoderma lucidum strains were inoculated into PD liquid fermentation medium for fermentation culture, the inoculum amount was 5%, the shaking table speed was 150 r / min, the temperature was 25°C, and the fermentation time was 8 days.

[0061] (3) Induction culture of Ganoderma lucidum cells by funga...

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Abstract

The invention relates to a fermentation process for improving the yield of lucid ganoderma laccase. The technology is characterized by comprising the following steps: using a lucid ganoderma cell liquid for culturing and adding fungal elicitors at the suitable stage of the cell growth; and after inducing for a certain time, obviously improving the yield of the lucid ganoderma laccase, wherein after inducing the fungal elicitors for 5-8 days, the enzyme activity of the lucid ganoderma laccase can reach 1068.4U / L at most which is 2.2 times of that of a control group. The invention can rapidly use the lucid ganoderma fermentation method to produce the laccase on a large scale; and the produced laccase can be used for waste water treatment, pulp bleaching, soil sanitation, dye decoloration, immunological detection and the like. The method has the advantages of simple technology, low cost, obvious effects and high yield of the lucid ganoderma laccase, and is a production method with industrialization prospects.

Description

technical field [0001] The invention relates to a preparation method of an industrial enzyme preparation, in particular to a fermentation method for improving the yield of ganoderma lucidum laccase. Background technique [0002] Laccase (laccase) is a class of copper-containing polyphenol oxidase (Ecl.10.3.2), which can oxidize a variety of phenols and aromatic amines. It has a wide range of applications [Hong Yuzhi et al. (2005) Bioengineering Journal 21: 547-552], and has become a hot spot in international enzyme engineering research in recent years. Laccase widely exists in plants, higher fungi, bacteria and insects, and can be obtained from many ways, but the most promising industrial production method is microbial fermentation. [0003] White rot fungi in basidiomycetes are a group of microorganisms with high laccase production, among which Ganoderma lucidum ( Ganoderma lucidum ) as an important medicinal fungus, people have carried out extensive research on its lacca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02C12R1/645C12R1/885
Inventor 姚强宫志远高兴喜任鹏飞任海霞李瑾曲玲王宗秀刘岩
Owner INST OF SOIL & FERTILIZER SHANDONG ACAD OF AGRI SCI