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Process method for removing residual arsenic, lead and copper in soy protein isolate

A technology of soybean protein isolate and process method, which is applied to the protein composition of vegetable seeds, protein food components, food science, etc., to achieve the effect of improving food safety

Active Publication Date: 2011-01-12
张东杰
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no report on the process of using chitosan solution as an adsorbent to remove residual arsenic, lead and copper in the process of soybean protein isolate

Method used

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  • Process method for removing residual arsenic, lead and copper in soy protein isolate
  • Process method for removing residual arsenic, lead and copper in soy protein isolate
  • Process method for removing residual arsenic, lead and copper in soy protein isolate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Adsorbent: Dissolve 10 g of chitosan with a deacylation degree of 95% in 1000 mL of 1% (v / v) acetic acid solution to prepare a 1% (w / v) chitosan solution.

[0025] 2. Process

[0026] (1) Alkaline extraction, first carry out alkaline extraction twice with low temperature and low denaturation soybean meal with sodium hydroxide. Alkaline extraction for the first time: Add water to the low-temperature and low-denatured soybean meal at a ratio of 1:10 by weight of material to liquid, mix well and use sodium hydroxide with a concentration of 20% (w / w) at a temperature of 50°C and a stirring rate of Under the condition of 60rpm, carry out alkaline extraction for 30min, and adjust the pH value of the solution to 7.0, then use a centrifuge for centrifugation at 3500-4500rpm, and add water to the filter residue according to the ratio of material to liquid weight ratio of 1:5, and then add a concentration of 20 % (w / w) of sodium hydroxide at a temperature of 50°C and a stirri...

Embodiment 2

[0035] One, adsorbent, with embodiment 1.

[0036] 2. Process

[0037] (1) Alkaline extraction, take low-temperature low-denatured soybean meal as raw material to carry out two alkali extractions, the ratio of liquid to liquid for the first alkali extraction is 1:8, the temperature is 45°C, the stirring speed is 60rpm, the alkali extraction is 50min, and when the pH value of the solution is 7.5, centrifuge Separation; the solid-liquid ratio of the second secondary alkali extraction is 1: 6, the temperature is 40° C., the stirring rate is 60 rpm, the alkali extraction is 30 min, and when the pH value of the solution is 7.5, after centrifugation, the two extracts are combined for subsequent use, and the others are the same as above.

[0038] (2) Acid precipitation, in the extract of (1), stirring speed 40rpm, add the hydrochloric acid that concentration is 0.01mol / L to adjust pH value to be 4.4, centrifuge, obtain protein precipitate for subsequent use, others are the same as ab...

Embodiment 3

[0047] One, adsorbent, with embodiment 1.

[0048] 2. Process

[0049] (1) Alkali extraction, take the raw material low-temperature and low-denaturation soybean meal for secondary alkali extraction. The solid-liquid ratio of the first alkaline extraction is 1:12, the alkaline extraction temperature is 50° C., the stirring rate is 80 rpm, and the alkaline extraction is 40 minutes. When the pH value of the solution is 7.5, centrifuge; the ratio of liquid to liquid for the second alkali extraction is 1:6, the temperature of alkali extraction is 50°C, the stirring rate is 80rpm, the alkali extraction is 10min, and when the pH value of the solution is 7.5, after centrifugal separation, two extractions The liquid is combined and set aside.

[0050] (2) acid precipitation, in the extract of (1), under stirring rate 40rpm, adding concentration is that 0.01mol / L hydrochloric acid adjusts pH value to be 4.6, centrifugal separation, obtains protein precipitate for subsequent use, other...

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Abstract

The invention discloses a process method for removing residual arsenic, lead and copper in soy protein isolate, which comprises the following steps of: by taking low-temperature low-degeneration bean pulp as a raw material, performing alkaline extraction and acid precipitation by taking 1 percent (weight in volume) solution of chitosan to obtain protein precipitate, adding water into the protein precipitate to prepare soy protein isolate liquid at the concentration of 10 to 15 percent (weight in weight), and homogenizing; and removing arsenic in the homogenized soy protein isolate liquid at the temperature of 20 to 30 DEG C when the pH value is 6.0 to 6.5, and performing absorption twice to remove lead and copper when the pH value is 6.5 to 7.0. In the method of the invention, after the soy protein isolate obtained by using the low-temperature low-degeneration bean pulp as the raw material alkaline and by extraction, acid precipitation and homogenizing is processed by absorption, analysis of the product by an atomic fluorescence spectrophotometer shows that: the arsenic content of the product is less than or equal to 0.008mg / kg and the arsenic adsorption rate is more than or equal to 99 percent; the analysis by an atomic absorption spectrophotometer shows that: the lead content is less than or equal to 0.006mg / kg and the lead adsorption rate is more than or equal to 99 percent; the analysis by the atomic absorption spectrophotometer shows that: the copper content is less than or equal to 3mg / kg and the copper adsorption rate is more than or equal to 75 percent; and thus, the edible safety of the soy protein isolate is improved.

Description

1. Technical field [0001] The invention relates to a deep processing technology of soybean protein isolate, in particular to a process for removing residual arsenic, lead and copper in soybean protein isolate. 2. Background technology [0002] At present, the production process of soybean protein isolate at home and abroad is mainly based on the alkali extraction and acid precipitation method. Because hydrochloric acid, sodium hydroxide, sodium pyrosulfite and other additives are used in the production process of soybean protein isolate, arsenic, Lead, copper, and the lead and copper contained in raw materials are enriched step by step during the production process of soybean protein isolate, which makes soybean protein isolate a potential safety hazard in eating. [0003] Arsenic is a non-essential element for the human body. The toxicity of arsenic is related to its chemical properties and valence state, and its toxicity is reflected by trivalent arsenic; lead has long-ter...

Claims

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Application Information

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IPC IPC(8): A23J1/14
Inventor 张东杰王颖张丽华
Owner 张东杰
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