Method for preparing corn seed coat dietary fiber
A technology of corn seed coat and dietary fiber, applied in food preparation, food science, application, etc., can solve the problems of reduced physiological activity of dietary fiber, poor flavor and color of hydrolyzed products, difficult control of product yield and quality, etc.
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Embodiment 1
[0015] Example 1: After the corn seed coat was pulverized, the particle size range was 10 to 30 meshes, and water was used as the extractant, the material-to-liquid ratio was 1:10, the temperature was 40 ° C, and the ultrasonic power was 600W. Ultrasonic treatment for 50 minutes; then in the microwave 300W condition Treat at lower temperature for 2 minutes, cool to 45°C, add 0.3% (w / w) mixed enzyme preparation of α-amylase and protease (the ratio of α-amylase and protease is 2:1), pH 5.0 Enzymatic hydrolysis under conditions for 30 minutes. After the enzymatic hydrolyzed feed solution was filtered, the filter residue was washed with deionized water, the filtrate was concentrated to remove protein, and then washed with alcohol.
Embodiment 2
[0016] Example 2: After the corn seed coat was pulverized, the particle size range was 20 to 40 meshes, and water was used as the extractant, the material-to-liquid ratio was 1:15, the temperature was 65 ° C, and the ultrasonic power was 400W. Ultrasonic treatment for 20 minutes; Treat at lower temperature for 3 minutes, cool to 50°C, add 0.5% (w / w) mixed enzyme preparation of α-amylase and protease (the ratio of α-amylase and protease is 1:2), pH 4.0 Enzymatic hydrolysis under conditions for 40 minutes. After the enzymatic hydrolyzed feed solution was filtered, the filter residue was washed with deionized water, the filtrate was concentrated to remove protein, and then washed with alcohol.
Embodiment 3
[0017] Example 3: After the corn seed coat was pulverized, the particle size range was 40 to 60 mesh, and water was used as the extractant, the ratio of material to liquid was 1:20, the temperature was 50 ° C, and the ultrasonic power was 200W. Ultrasonic treatment for 40 minutes; then in the microwave 400W condition Treat at lower temperature for 4 minutes, cool to 65°C, add 0.7% (w / w) mixed enzyme preparation of α-amylase and protease (the ratio of α-amylase and protease is 1:3), pH 4.5 Enzymatic hydrolysis under conditions for 50 minutes. After the enzymatic hydrolyzed feed solution was filtered, the filter residue was washed with deionized water, the filtrate was concentrated to remove protein, and then washed with alcohol.
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