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Near infrared spectrum identification method for pseudo-ginseng

A technology of near-infrared spectroscopy and Panax notoginseng, which is applied in the field of traditional Chinese medicine research, can solve problems such as complicated operation, long time consumption, and environmental pollution, and achieve the effect of accurate results, simple operation, and no pollution in the results

Active Publication Date: 2011-02-02
TIANJIN TASLY MORDEN TCM RESOURCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For identification by physical and chemical methods, it not only takes a long time and is complicated to operate, but also uses a large amount of organic reagents, causing environmental pollution

Method used

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  • Near infrared spectrum identification method for pseudo-ginseng
  • Near infrared spectrum identification method for pseudo-ginseng
  • Near infrared spectrum identification method for pseudo-ginseng

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] In this example, through more than 280 batches of near-infrared spectrograms of authentic Panax notoginseng and its common counterfeit products including curcuma, turmeric and turmeric, combined with chemometrics, a new identification model of Panax notoginseng was established. It is proved that the identification effect of the model is good.

[0039] 1. Instruments and materials

[0040] 1.1 Instrument

[0041] Antaris Fourier transform near-infrared spectrometer (Thermo Fisher, USA), SabIR diffuse reflection fiber optic probe accessories. Software: Result software (Thermo Fisher, USA) is used for spectrum acquisition, and TQ Analyst6.2 software (Thermo Fisher, USA) is used for spectral preprocessing and algorithm calculation.

[0042] 1.2 Sample source

[0043] The experimental samples included notoginseng medicinal materials from different origins such as Yunnan and common counterfeit notoginseng products such as zedoary, turmeric, and turmeric. The sources of sam...

Embodiment 2

[0063] In this example, the same instruments and methods as in Example 1 were used to establish a model and verify the identification rate of the model. The composition of the samples is also shown in Table 1. The difference between this embodiment and embodiment 1 is only:

[0064] 1. When collecting spectra in this embodiment, the raw medicinal materials were divided into 50 parts on average, 10 grams of each part were uniformly mixed, dried at 65°C for 8 hours, pulverized, and passed through a 60-mesh standard drug sieve. Then uniformly put them into 50ml standard sampling bottles, pad them tightly, and set them aside.

[0065] 2. In this embodiment, 25 is used as the principal component number to establish the identification model.

[0066] The model verified with the validation set found that the identification rate of the model was also 100%.

Embodiment 3

[0068] This embodiment uses the same instrument and modeling method as in Embodiment 1, the only difference lies in the composition of the sample. The genuine Panax notoginseng in the calibration set of this embodiment includes samples Nos. 1-52, 64-101, 110-132, and 139-155, excluding Sichuan Panax notoginseng with a sample number of 163-185; counterfeit Panax notoginseng includes samples 186-210 , No. 223-247, and No. 257-281 samples are the same as in Example 1. The genuine Panax notoginseng in the verification set includes samples 53-63, 102-109, 133-138, 156-162, and 163-185, including all Sichuan Panax notoginseng samples; - Sample No. 289, same as Example 1.

[0069] The identification results of the model obtained in this embodiment are shown in Table 3. After calculation, the identification rate of the model is 95%.

[0070] Table 3. Embodiment 3 identification results

[0071]

[0072]

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Abstract

The invention discloses a near infrared spectrum identification method for pseudo-ginseng, which belongs to the technical field of research into traditional Chinese medicines. The method comprises the following steps: (a) acquiring the near infrared spectra of set batches of qualified pseudo-ginseng, set batches of false pseudo-ginseng and samples to be identified; (b) establishing an identification model according to the near infrared spectra by using the discriminatory analysis method combining Mahalanobis Distance and principal component analysis; and (c) inputting the near infrared spectrum of the sample to be identified into the model to obtain the identification result. The qualified pseudo-ginseng preferably comprises 17-52 batches of pseudo-ginseng respectively from Wenshan in Yunnan, Honghe in Yunnan, Jingxi in Gangxi, Baise in Gangxi and Sichuan. The false pseudo-ginseng preferably comprises 6-12 batches of Rhizoma Zedoariae respectively from Gangxi and / or Fujian, radix curcumae from Gangxi and curcuma from Guangdong and / or Gangxi. The method can be used for identifying the pseudo-ginseng drug, and has the advantages of simple operation, high speed, accurate result and no pollution.

Description

technical field [0001] The invention relates to notoginseng, in particular to a method for identifying notoginseng based on near-infrared spectroscopy. It belongs to the technical field of traditional Chinese medicine research. Background technique [0002] Panax notoginseng is a plant of the genus Araliaceae, which was first recorded in "Compendium of Materia Medica". It is also known as Shentianqi, Tianqi ginseng, and Jinbuhuan. It is mainly distributed in Yunnan and Guangxi. It is a traditional traditional Chinese medicine. Panax notoginseng has the effects of stopping bleeding, dispelling blood stasis, reducing swelling and relieving pain. It is used for hematemesis, hemoptysis, chest and abdomen tingling, coronary heart disease and traumatic bleeding. It is known as the "Pearl of Chinese Medicine" (Zhang Hua. Pseudo-identification [J]. Modern Medicine and Health. 2007, 23(19): 2932). As a commonly used traditional Chinese medicinal material in clinical practice, due t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/49A61K36/258A61P7/04A61K125/00A61P9/10
Inventor 张延莹张金巍刘岩赵国磊张培
Owner TIANJIN TASLY MORDEN TCM RESOURCES
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