Gene combination, primer and probe used for detecting susceptibility of femoral head necrosis, and application

Abstract

The invention discloses a gene combination, a primer and a probe used for detecting the susceptibility of femoral head necrosis, and application. The gene combination comprises six genes, namely a VEGF gene of vascular endothelial growth factors, an FV gene and an MTHER gene of blood coagulation factors, a PON1 gene, an APOA1 gene and an APOB gene of lipid metabolism genes, which are closely related to the femoral head necrosis. The existence of a femoral head necrosis susceptibility gene of detected population is comprehensively detected and analyzed by detecting a group of genes and loci which are related to the susceptibility of the femoral head necrosis, utilizing a specific primer and the probe and combining single-nucleotide extending technology with microarray chip technology, and the femoral head necrosis susceptible population is screened from the population for changing improper living habits, so as to fulfill the aim of prevention.

Description

technical field [0001] The invention relates to a gene combination, a primer, a probe and its use, especially the gene combination for detecting the susceptibility to necrosis of the femoral head, its specific primers, probes and its use. Background technique [0002] Femoral head necrosis is often called avascular necrosis of femoral head or aseptic necrosis of femoral head because its main pathology is the blockage of blood supply to the femoral head and the destruction of the head bone caused by ischemia. Femoral head necrosis has a complex pathological process. If it cannot be treated promptly and effectively in the early stage, the femoral head will collapse, the joint space will be narrowed, and finally osteoarthritis will be caused. While suffering from physical pain, patients also suffer from psychological trauma, which also adds a heavy burden to families, units and society. [0003] Avascular necrosis of the femoral head (ANFH) is a common orthopedic disease, freq...

AI Technical Summary

Problems solved by technology

[0012] The technical problem to be solved by the present invention is that by detecting a group of genes and loci related to the susceptibility of femoral head necrosis, using specific primers and probes, combining single nucleotide extension technology with microarray chip technology, comprehensive detection and Analyze whether the tested population carries the "susceptibility gene of femoral head necrosis", screen out the susceptible population of femoral head necrosis from the crowd, change bad living habits, and achieve the purpose of prevention