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Method for improving foaming characteristic of draught beer

A beer and pure raw technology, which is applied in the field of beer manufacturing, can solve the problems of limited improvement, cumbersome process and high cost, and achieves the effects of convenient operation, wide application prospect and increasing the number of foams

Inactive Publication Date: 2011-02-16
ZHEJIANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Researchers have invented some methods for improving the foaminess of pure draft beer, such as extracting protease A inhibitors from Ganoderma lucidum fermentation broth and adding them to pure draft beer (CN200310106596.5); breeding strains that do not secrete protease A for use in Pure draft beer fermentation (CN200610155976.1); by adding biotin, Fe 3+ 、PO 4 3- At least one of them is to reduce the secretion of protease A (CN200810053644.1). The first method is complicated to extract inhibitors and the cost is high; the second method is cumbersome to prepare corresponding engineering bacteria; the third method is to reduce protease The secretion of A to improve the foaming performance of beer, the degree of improvement is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Add 100kg of cow's milk casein to 500 liters of water, adjust the pH to 7.0 with 10% sodium hydroxide solution under stirring, and after the casein is completely dissolved, add trypsin (1000 units / g casein) for enzymolysis During the enzymolysis process, keep the temperature of the hydrolyzate at 40°C and the pH at 7.0. When the pH value of the enzymolysis solution no longer drops, it means that the enzymolysis is over. Adjust the pH of the enzymolysis solution to 5.0 to terminate the reaction, and add casein 10% of diatomite by weight is 10kg. After the feed liquid is heated to 70°C, it is plate-frame filtered. The filtrate is sterilized by a microfiltration membrane, vacuum-concentrated to a sugar content of 25%, and then spray-dried to obtain a casein hydrolyzate powder. The powder is white, without burnt and other odors, and the N / P molar ratio is 33.

[0022] 2) During the production of pure draft beer, after beer post-fermentation is completed and before filtra...

Embodiment 2

[0025] 1) Add 100kg of cow's milk casein to 500 liters of water, adjust the pH to 7.0 with 10% sodium hydroxide solution under agitation, after the casein is completely dissolved, add trypsin (600 units / g casein) for hydrolysis During the hydrolysis, sodium hydroxide solution was added dropwise to keep the pH of the hydrolyzed solution at 7.0 and the temperature was maintained at 40°C. When the pH of the enzymolyzed solution no longer dropped, then add neutral protease (400 units / g casein) to reach a pH of 7.0 , At a temperature of 45°C, the same effect is applied until the pH no longer drops. Afterwards, adjust the pH of the hydrolyzate to 5.0 to terminate the reaction, add 10 kg of diatomaceous earth that is 10% of the weight of the casein, raise the temperature of the feed liquid to 70°C, and then filter the plate and frame, sterilize the filtrate through a microfiltration membrane, and concentrate it in vacuum to sugar 25%, and then spray-dried to obtain casein hydrolyzate...

Embodiment 3

[0029] 1) 100kg of casein is added to 500 liters of water, and the pH is adjusted to 7.0 with 10% sodium hydroxide solution under stirring. After the casein is completely dissolved, trypsin (1000 units / g casein) is added for enzymolysis. During the enzymolysis process, keep the temperature of the hydrolyzate at 40°C and the pH at 7.0. When the pH value of the enzymolysis solution no longer drops, it means that the enzymolysis is over. Adjust the pH of the enzymolysis solution to 5.0 to terminate the reaction, and add the weight of casein The 10% diatomite is 10kg, the material liquid is heated up to 70°C, and then plate and frame filtered, the filtrate is sterilized by microfiltration membrane, vacuum concentrated to 25% sugar content, and then spray-dried to obtain casein hydrolyzate powder. The powder is white, without burnt and other peculiar smells, and the N / P molar ratio is 38.

[0030] 2) During the production of pure draft beer, after beer post-fermentation is complete...

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Abstract

The invention discloses a method for improving the foaming characteristic of draught beer. The method comprises the following step of adding casein hydrolyzate into fermented and unfiltered draught beer and uniformly stirring the mixture; and the casein hydrolyzate is prepared by the following steps of: dissolving casein into water; adding protease; performing full enzymolysis and ending reaction; filtering; removing bacteria from filtrate; and concentrating and performing spray drying to prepare the casein hydrolyzate. By adding the casein hydrolyzate into the unfiltered draught beer, the number of foams of the draught beer is increased and the duration of the foaming of the beer after two months is increased, and the method is convenient to operate and has wide application prospect.

Description

technical field [0001] The invention relates to the technical field of beer production, in particular to a method for improving the foaminess of pure draft beer. Background technique [0002] Foam stability is an important quality characteristic of beer, which refers to the change characteristics of foam, such as foamability, foam retention, adhesion and appearance, during the shelf life. Pure draft beer has become a healthy drink favored by consumers, but pure draft beer has obvious defects in foam stability, that is, the foam stability gradually decreases with the prolongation of shelf life. [0003] Protease A is the key factor leading to the decline of the foam of pure draft beer (Wang Deliang, He Chunyan, Li Jianfei, He Xi, Li Huiping. Research on the relationship between protease A and pure draft beer foam stability. Beer Science and Technology, 2008, 12, 44-48) . Protease A is an acidic protease with a molecular weight of about 42 ku, located in the vacuoles of beer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12C5/02C12P21/06
Inventor 冯凤琴张辉陈锡威
Owner ZHEJIANG UNIV
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