Eucalyptus endophyte stain and use thereof in aluminum toxicity relief

An endophyte, eucalyptus technology, applied in applications, microorganism-based methods, chemicals for biological control, etc., to achieve the effect of reducing aluminum poisoning and improving the ability to resist aluminum poisoning

Inactive Publication Date: 2011-02-16
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Endophytic bacteria that can alleviate...

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  • Eucalyptus endophyte stain and use thereof in aluminum toxicity relief
  • Eucalyptus endophyte stain and use thereof in aluminum toxicity relief
  • Eucalyptus endophyte stain and use thereof in aluminum toxicity relief

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Embodiment 1

[0029] 1 Bacteria solution preparation

[0030] The separation and purification of bacterial strain 2 of the present invention include:

[0031] A, material: collect the roots of the wild Eucalyptus dunnen and Eucalyptus eucalyptus with a diameter of less than 0.5cm, stem segments and mature leaves with a diameter of less than 0.5cm;

[0032] B. Disinfection: leaves are sterilized by soaking in 70% alcohol for 40s and 0.1% mercury liter for 90s; stems are disinfected by soaking in 70% alcohol for 30s and 0.1% mercury liter for 60s; roots are disinfected by soaking in 70% alcohol for 50s and 0.1% mercury liter Soak for 120s;

[0033] C, separation: insert the roots, stem segments and mature leaves of the surface-sterilized wild Eucalyptus durnen and Eucalyptus macrophylla plants into the fungal solid medium to propagate endophytic fungi; the fungal solid medium is an improved Martin solid medium, The formula is peptone 5.0g / L, yeast extract powder 2.0g / L, glucose 20.0g / L, dip...

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Abstract

The invention provides a penicillium strain and use thereof. The eucalyptus endophyte strain is Penicilliumsp. strain 2 of which the collection number is CGMCC No.4278. When inoculated onto the eucalyptus, the eucalyptus endophytes can relieve the aluminum toxicity in the eucalyptus. In the invention, an eucalyptus endophyte strain is screened out by separation, the relieving effect and influences of the strain on aluminum toxicity are proved, and as the aluminum toxicity becomes more and more serious with a large area of soil in China suffering from damage of acid rain, the improvement on the aluminum toxicity resistance in plants by inoculating endophytic fungi onto the plants represents a new method, and thus, a new way for lowering the aluminum toxicity in the eucalyptus is found.

Description

technical field [0001] The invention relates to a eucalyptus endophyte and its application, more specifically to a eucalyptus endophyte and its application in alleviating aluminum poisoning. Background technique [0002] Aluminum is the most abundant metal in the earth's crust (approximately 7%) and the third most abundant element after oxygen (O) and silicon (Si). In recent years, due to the aggravation of acid rain pollution, many crops have been poisoned by aluminum. The area covered by acid rain in my country has exceeded 30% of the country's land area, and the aluminum in the soil is gradually released, seriously endangering the yield and quality of trees and crops [1-2] . [0003] Since Hartwell and Pember first discovered Al poisoning in the 1920s, the research on aluminum poisoning has been more than 90 years, but the mechanism of aluminum poisoning is still not very clear, the main reason is that aluminum has very complex chemical properties. Aluminum generally f...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P21/00C12N1/14C12R1/80
Inventor 洪伟吴承祯谢安强
Owner FUJIAN AGRI & FORESTRY UNIV
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