Eucalyptus endophyte stain and use thereof in aluminum toxicity relief
An endophyte, eucalyptus technology, applied in applications, microorganism-based methods, chemicals for biological control, etc., to achieve the effect of reducing aluminum poisoning and improving the ability to resist aluminum poisoning
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[0029] 1 Bacteria solution preparation
[0030] The separation and purification of bacterial strain 2 of the present invention include:
[0031] A, material: collect the roots of the wild Eucalyptus dunnen and Eucalyptus eucalyptus with a diameter of less than 0.5cm, stem segments and mature leaves with a diameter of less than 0.5cm;
[0032] B. Disinfection: leaves are sterilized by soaking in 70% alcohol for 40s and 0.1% mercury liter for 90s; stems are disinfected by soaking in 70% alcohol for 30s and 0.1% mercury liter for 60s; roots are disinfected by soaking in 70% alcohol for 50s and 0.1% mercury liter Soak for 120s;
[0033] C, separation: insert the roots, stem segments and mature leaves of the surface-sterilized wild Eucalyptus durnen and Eucalyptus macrophylla plants into the fungal solid medium to propagate endophytic fungi; the fungal solid medium is an improved Martin solid medium, The formula is peptone 5.0g / L, yeast extract powder 2.0g / L, glucose 20.0g / L, dip...
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