Ultrasound enzyme-assisted solanesol extracting method
An auxiliary extraction and solanesol technology, applied in the separation/purification of hydroxyl compounds, organic chemistry, fermentation, etc., can solve the problems of lye environmental pollution, easy degradation or isomerization of solanesol, and achieve waste liquid discharge less effect
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Embodiment 1
[0022] A kind of ultrasonic enzymatic method of the present invention assists the method for extracting solanesol, comprises the following steps:
[0023] 1) crush the dried potato leaves and pass through a 40-mesh sieve, then weigh 40 g of the dried potato leaves, add 600 ml of distilled water, ultrasonically wash with water for 30 minutes under the conditions of ultrasonic power 150W, frequency 40KHz, and temperature 30°C, and obtain solid substance A after filtration;
[0024] 2) Add 400ml of cellulase solution prepared with pH 4.0 disodium hydrogen phosphate-citric acid buffer solution with a concentration of 0.001g / ml to substance A, and add 400ml of cellulase solution under ultrasonic power of 100W, frequency of 40KHz, temperature of 50°C, and stirring speed of 120r Under the condition of ultrasound-assisted enzymolysis for 15 minutes, centrifuge at 4000r / min for 10 minutes, and discard the supernatant to obtain substance B;
[0025] 3) Add 200ml of lipase solution with ...
Embodiment 2
[0028] A kind of ultrasonic enzymatic method of the present invention assists the method for extracting solanesol, comprises the following steps:
[0029] 1) crush the dried potato leaves and pass through a 60-mesh sieve, then weigh 50 g of the potato leaves, add 250 ml of distilled water, and ultrasonically wash them for 40 minutes under the conditions of ultrasonic power 200W, frequency 40KHz, and temperature 40°C, and obtain solid substance A after filtration;
[0030] 2) Add 250ml of cellulase solution prepared with disodium hydrogen phosphate-citric acid buffer solution with pH 4.4 to the substance A, the concentration is 0.004g / ml, and the concentration is 150W, frequency 40KHz, temperature 40°C, stirring speed 110r Under the condition of ultrasound-assisted enzymolysis for 30 minutes, centrifuge at 3800r / min for 12 minutes, and discard the supernatant to obtain substance B;
[0031] 3) Add 500ml of lipase solution with a concentration of 0.004g / ml prepared with disodium...
Embodiment 3
[0034] A kind of ultrasonic enzymatic method of the present invention assists the method for extracting solanesol, comprises the following steps:
[0035] 1) crush the dried potato leaves and pass through a 50-mesh sieve, then weigh 20 g of potato leaves, add 400 ml of distilled water, ultrasonically wash for 50 minutes under the conditions of ultrasonic power 250W, frequency 40KHz, and temperature 50°C, and obtain solid substance A after filtration;
[0036]2) Add 300ml of cellulase solution prepared with disodium hydrogen phosphate-citric acid buffer solution with pH 4.8 to the substance A, the concentration is 0.002g / ml, and the concentration is 200W, frequency 40KHz, temperature 30℃, stirring speed 100r Under the condition of ultrasound-assisted enzymolysis for 45 minutes, centrifuge at 3600r / min for 15 minutes, and discard the supernatant to obtain substance B;
[0037] 3) Add 500ml of lipase solution with a concentration of 0.002g / ml prepared by disodium hydrogen phospha...
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