WecA gene knock-out train, its preparing method, application and method for screening M.tuberculosis (MTB) N-acetylglucosamine-1-phosphate transferase inhibitor

Abstract

N-acetylglucosamine-1-phosphate transferase is a key component for synthesis of cell wall of M.tuberculosis (MTB), and wec A gene is an encoding gene of the N-acetylglucosamine-1-phosphate transferase, the invention constructs a wec A gene knock-out strain MJ2008 (Mycobacterium smegmatis), CGMCC 3417 and takes the MJ2008 strain as a cell model for screening the N-acetylglucosamine-1-phosphate transferase in a high throughout so as to screen out the effective N-acetylglucosamine-1-phosphate transferase inhibitor from a combined compound library and the traditional Chinese medicine and prepare the anti-tuberculosis drug with a high efficiency; additionally, in human cells, there has no homologue of the MTB N-acetylglucosamine-1-phosphate transferase, accordingly, the developed anti-tuberculosis drug targeting the N-acetylglucosamine-1-phosphate transferase has no harm to the human body and overcomes the disadvantage of killing normal cells of antibacterial agents.

Description

Technical field: [0001] The present invention relates to a bacterial strain used for screening anti-tuberculosis drugs, especially a wecA gene knockout strain, a preparation method, a use and a method for screening Mycobacterium tuberculosis N-acetylglucosamine-1-phosphotransferase inhibitors . Background technique: [0002] Mycobacterium tuberculosis (M.tuberculosis) is the pathogenic bacterium that causes tuberculosis (tuberculosis), according to WHO reports [1] At present, nearly 1 / 3 of the people in the world have been infected with Mycobacterium tuberculosis, and there are about 90 million new tuberculosis patients every year. Due to the emergence of multi-drug resistant (MDR) strains and extensive drug-resistant (XDR) strains, some existing first-line and second-line anti-tuberculosis drugs cannot effectively cure tuberculosis [2] , resulting in approximately 2 million deaths from tuberculosis each year [3] , Tuberculosis has become one of the leading causes of deat...

AI Technical Summary

Problems solved by technology

However, N-acetylglucosamine-1-phosphate transferase has not been considered as the target of anti-tuberculosis drugs so far, and effective N-acetylglucosamine-1-phosphate has been screened from existing combinatorial compound libraries and traditional Chinese medicines. Related reports on transferase inhibitors