Turnover membrane bioreactor for use in micro-gravity environment

Abstract

The invention discloses a turnover membrane bioreactor for suspended biological cell multi-batch culture in a micro-gravity environment, which mainly comprises a bioreactor reactor, an air supply system, a liquid injection system, a stirring system, a circulating pump, a temperature, dissolved oxygen, pressure and pH parameter measuring control system, an exhaust system and a liquid drainage system. In the invention, the accurate quantification of the volume of injected or drained liquid is realized by controlling the working time and flow rate of a micro peristaltic pump, a porous hydrophobic breathable membrane is used to supply oxygen required by organisms for growth, the uniform distribution of the nutrients in the reactor is realized by a central stirring paddle, carbon dioxide, which is a waste gas from the growth metabolism of microbes, is exhausted under a negative pressure produced by a vacuum pump so as not to inhibit the microbes any longer, the drainage of culture waste liquid in the reactor in a micro-gravity environment is realized by using the turnover extrusion of a silicon rubber turnover membrane arranged in the reactor and the liquid drainage function of the peristaltic pump at the position of liquid drainage, a following culture test can be performed by automatically adding culture solution, and by repeating the previous operation, the multi-bath culture ofsuspended biological cells in the reactor is realized.

Description

technical field [0001] The invention relates to an inverted membrane bioreactor device for multi-batch culture of suspended biological cells, in particular to an inverted membrane bioreactor for microgravity conditions, mainly used in space stations, space laboratories, and space permanent bases (such as Long-term multi-batch culture and biological effect research of suspended biological cells in microgravity environments such as the moon and Mars bases). Background technique [0002] Space science research is one of the main fields of human space activities, and it is the inevitable development direction for human beings to explore the mysteries of the universe, obtain more natural resources, and expand living space. Carrying out life science research with clear scientific goals under space conditions is an important part of the development of space science in the world's aerospace powers. [0003] The space microgravity environment provides unprecedented opportunities for...

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to solve the technical problem that the biological cell culture device in the microgravity environment can only perform one-time culture and cannot meet the long-term and multi-batch culture research of suspended biological cells under the microgravity condition, and provides a simple structure , High culture efficiency, can realize multi-parameter real-time detection and automatic regulation of the culture system, can be used in microgravity environment for long-term multi-batch culture of suspended biological cells, multi-parameter real-time detection and automatic regulation flipped membrane bioreactor

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