Thermostatic hydroponic identification method for resistance of tobacco black shank at seedling stage

A tobacco black shank disease and identification method technology, applied in the field of warm water hydroponics identification of tobacco black shank resistance at the seedling stage, can solve the problems of long time and heavy workload, and achieve high repeatability, difficulty, and elimination of soil Effect of temperature difference

Active Publication Date: 2011-04-06
YUNNAN ACAD OF TOBACCO AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seedling identification method usually adopts zoospore or mycelium suspension to soak roots and inoculate. Artificial inoculation identification of living strains in disease nurseries is a commonly used identification method at present. The disadvantage is that inoculation after transplanting slow seedlings takes a long time and a large workload

Method used

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  • Thermostatic hydroponic identification method for resistance of tobacco black shank at seedling stage
  • Thermostatic hydroponic identification method for resistance of tobacco black shank at seedling stage
  • Thermostatic hydroponic identification method for resistance of tobacco black shank at seedling stage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: A method for identification of tobacco black shank resistance at seedling stage by constant temperature hydroponics, in which a floating pond with thermal insulation and heating is built in a glass greenhouse or a plastic greenhouse. The bottom of the pool is covered with a foamed plastic board about 4 cm thick, covered with 2 layers of plastic film, and the entire pool wall is surrounded by foamed plastic boards for insulation. One end of the seedling pond is equipped with a temperature-controlled electric heating rod (product of Zhongshan Chaoxin Electric Appliance Factory) and a pool water circulation pump (product of Guangdong Haili Group Co., Ltd.). The temperature control electric heating rod and the pool water circulation pump are linked through the temperature control device. The pool water is deep well water or tap water. The water depth is 10cm. According to the suitable onset temperature of black shank, the water temperature is set to 28°C. Afte...

Embodiment 2

[0026] Embodiment 2: Repeat Embodiment 1, with the following differences: the pool water temperature is set to 24°C.

Embodiment 3

[0027] Embodiment 3: Repeat Embodiment 1, with the following differences: the pool water temperature is set to 32°C.

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Abstract

The invention relates to a thermostatic hydroponic identification method for the resistance of tobacco black shank at a seedling stage. The method comprises the following steps of: establishing a water-insulating circulating floating pool; transplanting tobacco seedlings; preparing inoculum; and inoculating, investigating and grading illness states and estimating resistance. The invention has the advantages of rapid paroxysm, good and reliable result repetitiveness and no other disease interference, substitutes an identification result for a traditional land identification result at a seedling stage and can identify a great amount of materials in a small area for many times in a year, improve the result reliability, shorten the identification period and save the identification cost.

Description

technical field [0001] The invention belongs to the technical field of crop disease resistance identification, in particular to a warm water hydroponics identification method for tobacco black shank resistance at seedling stage. Background technique [0002] Tobacco black shank is one of the devastating diseases in the production of tobacco (Nicotianatabacum), and it is the most important factor restricting the production of tobacco leaves in various tobacco areas in my country. Cultivated tobacco. Tobacco leaves lost due to tobacco black shank accounted for about 1% to 2% of the total output in North Carolina, USA. In 1995 and 1996, the losses caused by black shank reached 36.1 million US dollars, accounting for the total loss of the disease. 35.13%, ranking first among all diseases. The disease has a wide distribution in my country, except Heilongjiang Province, which has not yet been found, and other tobacco-growing provinces (including Taiwan Province) have occurred. T...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G31/00C12N1/14C12R1/645
Inventor 李梅云刘勇冷晓东李永平肖炳光卢秀萍李祥
Owner YUNNAN ACAD OF TOBACCO AGRI SCI
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