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Application of osthole in preparation of medicament for preventing and treating pulmonary fibrosis

A technology of osthole and pulmonary fibrosis, which is applied in the field of medicine, can solve the problems of pulmonary fibrosis drugs that have not yet been seen, and achieve the effect of single active ingredient, convenient use and definite curative effect

Active Publication Date: 2013-04-03
JIANGSU SUDA INVESTMENT CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is mainly used for anti-inflammation and antibacterial clinically, and there is no report about it as a drug for preventing and treating pulmonary fibrosis.

Method used

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  • Application of osthole in preparation of medicament for preventing and treating pulmonary fibrosis
  • Application of osthole in preparation of medicament for preventing and treating pulmonary fibrosis
  • Application of osthole in preparation of medicament for preventing and treating pulmonary fibrosis

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0040] Preparation of lung tissue homogenate: weigh the rest of the lung tissue, add 9 times the weight of the lung tissue in ice saline, make a 10% lung tissue homogenate with an endotomy tissue homogenizer, and store it in a -80°C refrigerator to measure T -AOC, MDA, GSH biochemical indicators.

[0041] Serum preparation: After the whole blood was collected from the abdominal aorta of the rats in each group, it was centrifuged at 3500r / min for 10min, and the supernatant was carefully taken out and placed in a 1.5ml centrifuge tube. The transferred supernatant was centrifuged again at 3500r / min for 10min. The prepared serum was stored in a refrigerator at -80°C to determine the biochemical indicators of T-AOC, MDA and GSH in the serum.

[0042] 3. Lung coefficient

[0043] The lung coefficient was calculated according to the following formula, lung coefficient=lung weight (mg) / body weight (g). Because a large number of inflammatory cells exudate in the early stage of pulmon...

Embodiment 1

[0051] Example 1 Effect of osthole on lung histomorphology in rats with pulmonary fibrosis

[0052] The lung tissues of the experimental rats were sectioned with paraffin and stained with HE. The observation results under the light microscope showed that the lung tissue structure of the normal group was clear, the alveolar cavity was clear, the alveolar wall was intact, and there was no thickening of the alveolar septum and no inflammatory cell infiltration. In the model group, the structure of the lung tissue was disordered, the infiltration of inflammatory cells was extensive, the alveolar space was narrowed or disappeared, the alveolar septum was significantly thickened, a large number of fibroblasts proliferated and collagen fibers were deposited in the pulmonary interstitium, and alveolitis was significant (P Figure 1~6 .

[0053] Table 1 Effect of osthole on lung histomorphology in rats with pulmonary fibrosis

[0054]

[0055] ##P<0.01vs control; *P<0.05, **P<0.01,...

Embodiment 2

[0056] Example 2 Effect of osthole on body weight and lung coefficient of pulmonary fibrosis rats

[0057] After the animal model was established, the body weight of the rats in the model group was significantly lower than that of the normal group (P<0.001), and the body weight of the rats in the prednisone group and the high-dose osthole group were significantly increased compared with the model group (P<0.01). Compared with the model group, the rats in the middle and small doses of beddingtin increased significantly in body weight (P<0.001); the lung coefficient of the rats in the model group was significantly higher than that in the normal control group (P<0.001); Compared with the lung coefficient, the lung coefficient of the rats in the large and medium dose groups of osthole and the rats in the prednisone acetate group were all decreased (P<0.05), and the lung coefficient of the rats in the small dose of osthole group was significantly reduced (P<0.05). 0.01). See Table...

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Abstract

The invention relates to application of osthole in the preparation of a medicament for preventing and treating pulmonary fibrosis. A pulmonary fibrosis animal model of a rat is replicated through dripping BLM (Bleomycin) into an air tube; the osthole is selected as a treatment medicament for the first time to research the influence of the osthole on lung tissues and serum-related biochemical indicators and pathological variations of the pulmonary fibrosis rat, and the treating effect of the osthole on the pulmonary fibrosis rat model is observed, therefore, experiment basis is provided for pulmonary fibrosis preventing and treating effects of the osthol.

Description

technical field [0001] The invention relates to the application of osthole in the preparation of medicines for preventing and treating pulmonary fibrosis, which belongs to the technical field of medicine Background technique [0002] Pulmonary (interstitial) fibrosis (PF) is a diffuse alveolitis and alveolar structure disorder of unknown cause, which leads to alveolar and pulmonary interstitial fibrosis, and eventually respiratory failure. It is also caused by a variety of reasons. The common outcome of acute and chronic lung diseases is a pulmonary obstructive disease that seriously endangers human health. The clinical research on pulmonary fibrosis mainly focuses on idiopathic pulmonary fibrosis (IPF), which is characterized by abnormal injury of alveolar epithelial cells, fibroblast proliferation and extracellular matrix remodeling. The main pathological changes can lead to the loss of lung tissue function, eventually causing respiratory failure and fatal lung interstiti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/37A61P11/00
Inventor 顾振纶蒋小岗崔红霞郭次仪周忠继
Owner JIANGSU SUDA INVESTMENT CO LTD
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