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Maize pollen tissue specific promoter and expression vector thereof

A tissue-specific, maize pollen-based technology, applied in the field of plant molecular biology and genetic engineering, can solve the problems of incomplete reliability and laboriousness

Inactive Publication Date: 2011-04-13
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are various techniques for producing male sterility. One is to manually or mechanically remove the anthers or spikes of the female parent plant, but this method is laborious and not entirely reliable

Method used

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  • Maize pollen tissue specific promoter and expression vector thereof
  • Maize pollen tissue specific promoter and expression vector thereof
  • Maize pollen tissue specific promoter and expression vector thereof

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Experimental program
Comparison scheme
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Embodiment approach

[0019] 1. Genes specifically expressed in maize pollen tissue PAB5 the acquisition

[0020] First, search for the gene with the keyword "PAB5" on the NCBI website (http: / / www.ncbi.nlm.nih.gov) to obtain the Arabidopsis PAB5 The protein sequence of the gene. A local corn protein database was established, and the obtained protein sequence of Arabidopsis thaliana PAB5 gene was compared by BlastP (E-value=0.001) using the "DNATOOLS 6.0" software, and the corn candidate protein sequence with the highest homology was screened out. Then, the "DNATOOLS 6.0" software was used to establish a local database of the obtained maize genome DNA sequence data, and the obtained maize protein sequence was compared with BlastN (E-value=0.001) to screen out the candidate genes with the highest homology.

[0021] GUS tissue staining was performed on the transgenic plants, but the expression of GUS was detected in the floral organs and immature seeds of Arabidopsis. It was speculated that GUS may ...

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Abstract

The invention discloses a maize pollen tissue specific promoter and an expression vector thereof, and the promoter is 2222bp long, comprises a sequence under the (400) 1 item in a sequence table and is used for replacing a CaMV35S promoter on the plant expression vector pCAMBIA1301 so as to construct the new plant expression vector named p-ZMP5. By means of the obtainment of the promoter, conditions for utilizing genetic engineering to obtain a plant male sterile line and a restoring line can be created, the basis for genetic engineering research is provided and the foundation for specific expression of exogenous genes in pollen is provided, wherein the exogenous genes can be coding genes for killing insect toxin (targeting insects which take the pollen as feed), the genes which can enhance or modify the nutritional value of the pollen and the like. In addition, by interfering with PAB5 genes through RNAi (ribonucleic acid interference), the prevention of the generation of the pollen can be applied in street trees, thereby having a certain alleviating role and the like for pollen allergy and having important theoretical and practical significance.

Description

technical field [0001] The invention belongs to the fields of plant molecular biology and genetic engineering, and specifically relates to a corn poly(A) binding protein PAB5 The acquisition of gene promoters and the construction of plant expression vectors are particularly related to inducing the specific expression of foreign genes in pollen and obtaining plant male sterile lines and restorer lines. Background technique [0002] Maize belongs to the Poaceae Maize genus. The sown area of ​​corn in the world ranks third after wheat and rice. Corn is used as feed, food and industrial raw material, and is a staple food in many regions. In addition to eating, corn is also the main raw material for industrial alcohol and shochu. Therefore, the research on maize is of great significance. [0003] Anther is the place where pollen develops, and plant male sterile line is an important way to utilize crop heterosis, so the research on anther and pollen development has important a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/82A01H4/00A01H5/00
Inventor 程备久朱苏文项艳郑丽莉周玉琼
Owner ANHUI AGRICULTURAL UNIVERSITY
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