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Tissue engineered cardiac muscle using OPF (Oligo(Poly(Ethylene Glycol) Fumarate) hydrogel as a carrier and preparation method thereof

A tissue engineering and hydrogel technology, applied in the direction of prosthesis, medical science, etc., can solve the problems of application limitation, embryonic stem cell ethics and immune rejection, and achieve the advantages of convenient operation, avoiding difficulty in material source, and avoiding immune rejection. Effect

Inactive Publication Date: 2011-05-04
INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of embryonic stem cells is limited due to ethical and moral issues and immune rejection.

Method used

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  • Tissue engineered cardiac muscle using OPF (Oligo(Poly(Ethylene Glycol) Fumarate) hydrogel as a carrier and preparation method thereof
  • Tissue engineered cardiac muscle using OPF (Oligo(Poly(Ethylene Glycol) Fumarate) hydrogel as a carrier and preparation method thereof
  • Tissue engineered cardiac muscle using OPF (Oligo(Poly(Ethylene Glycol) Fumarate) hydrogel as a carrier and preparation method thereof

Examples

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Effect test

Embodiment 1

[0029] Example 1: Preparation of tissue-engineered myocardium using OPF hydrogel as carrier in the present invention

[0030] Proceed as follows:

[0031] (1) Preparation of OPF:

[0032]Dissolve 30 g of polyethylene glycol with a molecular weight of 10 kDa in 250 ml of toluene, and after azeotropic distillation, dissolve the polyethylene glycol in 250 mL of anhydrous dichloromethane to obtain a polyethylene glycol solution; Maleyl chloride (0.3mol) and triethylamine were added dropwise to the polyethylene glycol solution, and the reaction flask was maintained at 0°C during the dropwise addition, and then the reactant was stirred at room temperature for 1 to 2 days; then the dichloromethane was removed by rotary evaporation , add 500mL ethyl acetate to dissolve the reaction product; filter to remove the salt formed by chloride ions and triethylamine in the reaction; recrystallize the product twice in ethyl acetate; add ether to precipitate the final purified OPF. Vacuum dryi...

Embodiment 2

[0038] Example 2: Tissue engineered myocardial transplantation and cardiac function testing

[0039] (1) Preparation of animal myocardial infarction model:

[0040] The experimental S-D rats (about 225-300g) were intraperitoneally anesthetized with sodium pentobarbital (40 mg / kg body weight), and connected to an animal microventilator. A thoracotomy was performed, and the pericardium was cut to fully expose the heart; 0-7 silk sutures were used to suture and ligate 2 mm below the left atrial appendage; after ligation, the left ventricular wall became pale and the wall motion weakened. ECG monitoring ((see image 3 )) See that the ST segments of leads I and II are significantly elevated, indicating that the animal model of coronary artery infarction has been successfully prepared.

[0041] (2) Transplantation of tissue-engineered myocardium and detection of cardiac function

[0042] Forty S-D rats successfully prepared from the myocardial infarction model in step (1) were ra...

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Abstract

The invention belongs to the field of tissue engineering and regenerative medicine, and discloses an injectable tissue engineered cardiac muscle which uses low oligo(poly(ethylene glycol) fumarate (OPF) hydrogel as a support material and contains OPF hydrogel and iPS (Induced Pluripotent Stem) cells or cardiac muscle cells originating from the iPS cells. A preparation method of the tissue engineered cardiac muscle specifically comprises the steps of: adding the OPF and a crosslinking agent into a PBS (Poly-Butylene Succinate) solution; then adding an ammonium persulfate solution and a TEMED (Tetramethylethylenediamine) solution; and adding PBS suspension containing the iPS cells and slightly and uniformly mixing. The invention also discloses the preparation method and application of the tissue engineered cardiac muscle. In the invention, by adopting the OPF hydrogel as a carrier, the detension ratio and the survival ratio of the iPS cell are improved and a good repairing effect on heart functions of an infarcted zone is good; secondly, the tissue engineered cardiac muscle disclosed by the invention can be injected so that the operation is convenient; and in addition, because the iPS cells are used as seed cells, the problems on ethical morals and immunological rejection do not exist. In the invention, the preparation method is simple and the implementation conditions are mild.

Description

technical field [0001] The invention belongs to the field of tissue engineering and regenerative medicine, and in particular relates to an injectable tissue-engineered myocardium using oligoethylene glycol fumarate (OPF) hydrogel as a scaffold material, and a preparation method of the tissue-engineered myocardium. Background technique [0002] Ischemic heart disease causes myocardial necrosis, which in turn leads to heart failure, with high morbidity and mortality. Stem cell transplantation can repair damaged myocardial tissue and improve heart function to a certain extent. However, the low retention rate of cells injected into the myocardial infarction site and cell death after transplantation limit the application of this method. Utilizing injectable tissue engineered myocardium, injecting stem cells and injectable scaffold materials directly into myocardial injury sites for repair of damage can effectively solve the problems faced by pure stem cell transplantation (Wang ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/18C08G65/48
Inventor 王常勇王海滨刘志强段翠密林秋霞
Owner INST OF BASIC MEDICAL SCI ACAD OF MILITARY MEDICAL SCI OF PLA
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