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Rhizopus strains, yeast strains, distiller's yeast containing same and production method for distiller's yeast

A technology of Saccharomyces cerevisiae and Rhizopus oryzae, which is applied in the field of yeast strains and Rhizopus oryzae, can solve the problems that microorganisms cannot adapt well to each other, the taste cannot meet the needs of customers, and the taste of products cannot be adjusted, so as to achieve the effect of guaranteeing standardized control

Active Publication Date: 2011-05-04
ANGELYEAST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The prior art has the following disadvantages: a) each microorganism is purely cultured separately and then mixed, and when the mixed product is used by the user, each microorganism cannot adapt well to each other; b) the taste of the blended product of the prior art is usually not good Meet the needs of customers, and can not adjust the taste of products according to the different needs of various customers

Method used

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  • Rhizopus strains, yeast strains, distiller's yeast containing same and production method for distiller's yeast
  • Rhizopus strains, yeast strains, distiller's yeast containing same and production method for distiller's yeast
  • Rhizopus strains, yeast strains, distiller's yeast containing same and production method for distiller's yeast

Examples

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Effect test

Embodiment 1

[0167] Example 1 uses Rhizopus A (Rizopus oryzae CCTCC M209191) and yeast 21 (Saccharomyces cerevisiae CCTCC M209190) of the present invention.

[0168] A) Production of finished song

[0169] (1) Expanded culture of yeast strains

[0170] Firstly, the yeast liquid complete medium is prepared, and the components of the yeast liquid complete medium are: 10% sucrose, 2% yeast autodissolving powder, 0.1% potassium dihydrogen phosphate, 0.1% magnesium sulfate, and pH: 4.8.

[0171] Then inoculate, pick a ring of thallus from the yeast slant strain and transfer it into the prepared liquid Erlenmeyer flask, culture on a shaker at 30°C and 200rpm for 36 hours, and centrifugally filter to obtain a yeast filter cake for later use.

[0172] (2) the cultivation of rhizopus bran species

[0173] Prepare 80% water-containing bran, divide into 1000mL triangular flasks, fill each bottle with 150g, sterilize at 121°C, 0.1MPa, and sterilize for 40 minutes; take it out and cool it for later u...

Embodiment 2

[0184] Example 2 uses Rhizopus A (Rizopus oryzae CCTCC M209191) and yeast 21 (Saccharomyces cerevisiae CCTCC M209190) of the present invention.

[0185] A) Production of finished song

[0186] (1) Expanded culture of yeast strains

[0187] First prepare the complete yeast liquid medium, then inoculate, pick a ring of bacteria from the yeast slant and put it into the prepared liquid Erlenmeyer flask, culture it on a shaking table at 29°C and 200rpm for 48 hours, and centrifugally filter to get the yeast filter cake for later use .

[0188] (2) the cultivation of rhizopus bran species

[0189] Prepare 75% water-containing bran, divide into 1000mL triangular flasks, fill each bottle with 165g, sterilize at 121°C, 0.1MPa, and sterilize for 50 minutes; take it out and cool it for later use; then insert the original bran seed into the sterilized culture In the medium, each Erlenmeyer flask received 6 slices and placed in a 28°C incubator for 72 hours.

[0190] (3) Preparation of...

Embodiment 3

[0198] Except adopting rhizopus B, yeast 23, A) the production of finished song and B) the brewing of rice wine are identical with embodiment 1.

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Abstract

The invention relates to rhizopus strains, yeast strains, distiller's yeast containing the same and a production method for the distiller's yeast. The invention relates to rhizopus oryzae strains CCTCC No: M209191 and CCTCC No: M209232, saccharomyces cerevisiae strains CCTCC No: M209190 and CCTCC No: M209231, and rice distiller's yeast containing the strains. The method for producing the rice distiller's yeast comprises the following steps of: inoculating the rhizopus strains and the yeast strains into a culture medium, and performing mixed culture to obtain mixed culture yeast; inoculating the rhizopus strains into the culture medium, and performing rhizopus culture to obtain pure yeast; and blending the pure yeast and the mixed culture yeast in a certain ratio to obtain the rice distiller's yeast. The rhizopus strains and the yeast strains have good adaptability during mixed culture. The method can meet different requirements of customers on mouthfeel, and meanwhile guarantees standard control of product quality.

Description

technical field [0001] The invention relates to a rhizopus strain, a yeast strain, a distiller's yeast containing the rhizopus and yeast and a production method of the distiller's yeast, in particular to a rhizopus strain and a yeast strain with good mutual adaptability during mixed culture. Background technique [0002] The original distiller's yeast is moldy or germinated grains, and people improve it to make distiller's yeast suitable for winemaking. Roughly in the Song Dynasty, the types and manufacturing techniques of Chinese distiller's yeast were basically finalized. Modern distiller's yeast is still widely used in the brewing of rice wine, white wine, etc. Due to the different raw materials and production methods used, the natural conditions of the production areas are different, and the varieties of distiller's yeast are rich and colorful. [0003] Sweet wine is made by saccharifying and fermenting steamed rice with sweet wine koji. The flavor and nutrition of swe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/18C12G3/02C12R1/865C12R1/845
CPCC12G1/0203C12G2200/05C12N1/14C12N1/18C12N1/145C12N1/185C12R2001/845C12R2001/865
Inventor 俞学锋李知洪余明华姚鹃郑国斌周立学李梅忠
Owner ANGELYEAST CO LTD
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