Method for extracting peanut protein
An extraction method, peanut protein technology, applied in the protein composition of vegetable seeds, protein food ingredients, food science, etc., can solve the problems of long separation time, high separation temperature, variability of peanut protein isolate, etc., and achieve low production cost , the effect of short extraction time
Inactive Publication Date: 2011-05-11
SHANDONG PEANUT RES INST
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Problems solved by technology
[0004] The problem to be solved by the present invention is to provide a method for preparing peanut protein isolate with low denaturation, so as to overcome the phenomenon that under the existing conditions, peanut protein isolate is easy to denature, the separation time is long, the separation temperature is high, the yield of protein isolate is low, and the purity is low.
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Embodiment 1
[0035] Low-denatured peanut protein powder, PH value 9 alkali soluble, solid-liquid ratio 1:10, power 210W, ultrasonic frequency 28HZ, temperature 35°C, ultrasonic extraction for 25min, centrifugation, secondary ultrasonic extraction, combined supernatant solution, pH 4.5 hydrochloric acid precipitation, centrifugation, take the precipitate, eluted twice with 95% ethanol, then washed three times with distilled water, freeze-dried, peanut protein isolate powder, the extraction rate was 80.09% by Kjeldahl azotometer %.
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The invention discloses a method for extracting peanut protein, which comprises the following steps of: uniformly dissolving low-denatured peanut protein powder in alkali, performing ultrasonic extraction, centrifuging, performing ultrasonic extraction again, mixing supernatant, performing acid precipitation, centrifuging, washing precipitates with alcohol for two times, washing the precipitates with water for three times, and freeze-drying to obtain peanut protein isolate powder. The method has the advantages of short extraction time and low production cost.
Description
Technical field: [0001] The invention relates to a method for extracting low-denatured peanut protein isolate from low-temperature cold-pressed peanut protein powder by using an alkali extraction and acid precipitation method under ultrasonic-assisted conditions, and belongs to the technical field of peanut protein isolate processing methods. Background technique: [0002] Peanut is one of the six major oil crops in China, and its output ranks first in the world. According to "Compendium of Materia Medica Supplements", peanuts delight the spleen and stomach, moisten the lungs and resolve phlegm, nourish qi, clear the throat and relieve itching. "Southern Yunnan Materia Medica" contains: boiled peanuts in salt water to treat pulmonary tuberculosis, fried with dry fire to promote blood circulation, and to treat stomach pain due to cold accumulation in the abdomen. "Medicinal Test" contains: This product is fried and eaten to clear the stomach and refresh the spleen, smooth th...
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Inventor 杨庆利高俊安张会翠于丽娜朱凤张初署孙杰毕杰
Owner SHANDONG PEANUT RES INST