Organism fluorescent three-dimensional imaging system and application thereof

Abstract

The invention provides an organism fluorescent three-dimensional imaging system comprising a camera obscura, an illumination module, a rotating-platform device, a charge coupled device (CCD) imaging system, a computer and an anaesthesia device, wherein, the illumination module comprises a laser, a beam splitting fiber, a lighting (light emitting diode) LED and an illumination module controller; the rotating-platform device comprises an anaesthesia apparatus, a temperature control device, a rotating motor and a rotating-platform controller; the CCD imaging system comprises a CCD device, an electric focusing camera lens, a bandpass filter and a CCD imaging system controller; and a total beam end of the beam splitting fiber is connected with an output end of the laser; a beam splitting end of the beam splitting fiber is positioned above a rotating platform; the lighting LED is positioned above a biological rotating platform; and the optical axis of a receiving optical path of the CCD imaging system is crossed with the center of the circle of the biological rotating platform. In the invention, the beam splitting fiber is adopted to stimulate the uniform illumination to the whole organism, thus realizing the continuity and the instantaneity of high flux data collection, eliminating the displacement of the organism organ caused by the suspended rotation, and realizing high sensitivity and accurate image data collection.

Description

technical field [0001] The invention belongs to the technical field of fluorescence three-dimensional imaging, and relates to a biofluorescence three-dimensional imaging system and its application. Background technique [0002] With the application of various technologies in the field of molecular imaging in modern life science research, biological imaging technology has received more and more attention. Biological imaging technology refers to the application of imaging methods to conduct qualitative and quantitative research on biological processes in vivo at the tissue, cellular, and molecular levels, which enables the study of life processes to be carried out in vivo. We can use various ultrasonic waves (ultrasound imaging technology) or electromagnetic waves (CT) or electromagnetic fields (MRI) or high-energy particles (PET) that can penetrate organisms and interact with certain biological components without damaging animals. Under the premise of long-term longitudinal ...

AI Technical Summary

Problems solved by technology

In addition, the scanning point excitation light source greatly reduces the efficiency of data acquisition, so it takes a long time to collect 360° panoramic images, which seriously affects the real-time performance of detection

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