Organism fluorescent three-dimensional imaging system and application thereof

An imaging system, fluorescent three-dimensional technology, applied in applications, medical science, sensors, etc., can solve the problems of reducing data acquisition efficiency, affecting real-time performance, and taking a long time to achieve the elimination of organ displacement and high-throughput data acquisition , Realize the effect of continuity and real-time

Inactive Publication Date: 2011-05-11
ZHEJIANG UNIV
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  • Application Information

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Problems solved by technology

In addition, the scanning point excitation light source greatly reduces the efficiency of data acquisition, so ...

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  • Organism fluorescent three-dimensional imaging system and application thereof
  • Organism fluorescent three-dimensional imaging system and application thereof
  • Organism fluorescent three-dimensional imaging system and application thereof

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Embodiment 2

[0017] Embodiment 2 Concrete operation of the present invention

[0018] 1. Preparatory stage: put the organism with fluorescent markers into the rotating platform 3 in the dark box 1, put its head into the anesthesia device 31, and turn on the anesthesia device 6 to enter the anesthesia state.

[0019] 2. Bright field image acquisition: turn on the lighting LED 23, rotate the bandpass filter wheel 43 to select a suitable filter, adjust the field of view of the electric focus lens 42, rotate the rotating platform 3 at a constant speed of 360°, and collect the rotation process of the platform by the CCD device 41 The images of the organism under bright field conditions at multiple angles are transmitted to the computer 5 to complete the reconstruction of the outline of the organism.

[0020] 3. Dark field image acquisition: turn off the lighting LED 23, turn on the laser 21, excite the fluorescent markers in the living body to emit light, rotate the biological rotating platf...

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Abstract

The invention provides an organism fluorescent three-dimensional imaging system comprising a camera obscura, an illumination module, a rotating-platform device, a charge coupled device (CCD) imaging system, a computer and an anaesthesia device, wherein, the illumination module comprises a laser, a beam splitting fiber, a lighting (light emitting diode) LED and an illumination module controller; the rotating-platform device comprises an anaesthesia apparatus, a temperature control device, a rotating motor and a rotating-platform controller; the CCD imaging system comprises a CCD device, an electric focusing camera lens, a bandpass filter and a CCD imaging system controller; and a total beam end of the beam splitting fiber is connected with an output end of the laser; a beam splitting end of the beam splitting fiber is positioned above a rotating platform; the lighting LED is positioned above a biological rotating platform; and the optical axis of a receiving optical path of the CCD imaging system is crossed with the center of the circle of the biological rotating platform. In the invention, the beam splitting fiber is adopted to stimulate the uniform illumination to the whole organism, thus realizing the continuity and the instantaneity of high flux data collection, eliminating the displacement of the organism organ caused by the suspended rotation, and realizing high sensitivity and accurate image data collection.

Description

technical field [0001] The invention belongs to the technical field of fluorescence three-dimensional imaging, and relates to a biofluorescence three-dimensional imaging system and its application. Background technique [0002] With the application of various technologies in the field of molecular imaging in modern life science research, biological imaging technology has received more and more attention. Biological imaging technology refers to the application of imaging methods to conduct qualitative and quantitative research on biological processes in vivo at the tissue, cellular, and molecular levels, which enables the study of life processes to be carried out in vivo. We can use various ultrasonic waves (ultrasound imaging technology) or electromagnetic waves (CT) or electromagnetic fields (MRI) or high-energy particles (PET) that can penetrate organisms and interact with certain biological components without damaging animals. Under the premise of long-term longitudinal ...

Claims

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Application Information

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IPC IPC(8): A61B5/00
Inventor 牟颖王莹宋博凡张君金伟金钦汉
Owner ZHEJIANG UNIV
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