Transplanted wild ginseng ginsenoside B as well as extraction method and medical application thereof
A ginsenoside and glucopyranose-based technology, applied in the field of ginseng saponin B and its extraction, can solve the problems of forest resource reduction, ecological environment damage, water and soil erosion, etc., and achieve remarkable curative effect
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Embodiment 1
[0021] a, extract: get dry ginseng root 1.4kg, pulverize, and be 65% (V / V) ethanol heating reflux extraction 3 times with concentration, be respectively 3h, 2h, 1h each time, filter, merge extract, reduce Concentrate under reduced pressure to a relative density of 1.12 to 1.15, extract with petroleum ether, chloroform, ethyl acetate, and n-butanol in sequence, and recover the solvent under reduced pressure to dryness to obtain 110 g of n-butanol;
[0022] b. Separation: the obtained n-butanol part is separated by column chromatography through silica gel with a specification of 200 to 300 meshes, and eluted with an eluent of chloroform:methanol (V / V)=8:1 , collecting part of the eluate from undergrowth ginseng saponin B, and recovering the solvent to obtain a thick product of undergrowth ginsenoside B, with a yield of 0.019%;
[0023] c. Purification of undergrowth ginsenoside B: the isolated crude undergrowth ginsenoside B was purified by recrystallization to obtain pure under...
Embodiment 2
[0033] a, extract: get dry ginseng root 1.4kg, pulverize, and be 75% ethanol (V / V) heat reflux extraction 3 times with concentration, be respectively 3h, 2h, 1h each time, filter, merge extract, reduce Concentrate under reduced pressure to a relative density of 1.12 to 1.15, extract with petroleum ether, chloroform, ethyl acetate, and n-butanol in sequence, and recover the solvent under reduced pressure to dryness to obtain 120 g of n-butanol;
[0034] b. Separation: The obtained n-butanol part is separated by column chromatography through silica gel with a specification of 200 to 300 meshes, and eluted with an eluent of chloroform: methanol (V / V)=2:1 , collecting part of the eluate from undergrowth ginseng saponin B, recovering the solvent to obtain the thick product of undergrowth ginsenoside B, with a yield of 0.013%;
[0035] c. Purification of undergrowth ginsenoside B: the separated crude undergrowth ginsenoside B was purified again by column chromatography to obtain pur...
Embodiment 3
[0037] a, extraction: get the dried ginseng root 1.4kg, pulverize, and extract 3 times under reflux with 85% ethanol (V / V) with a concentration, each time is respectively 3h, 2h, 1h, filters, and merges the extracts, Concentrate under reduced pressure to a relative density of 1.12 to 1.15, extract with petroleum ether, chloroform, ethyl acetate, and n-butanol in sequence, and recover the solvent under reduced pressure to dryness to obtain 100 g of n-butanol.
[0038] b. Separation: The obtained n-butanol part is separated by column chromatography through silica gel with a specification of 200 to 300 meshes, and eluted with an eluent of chloroform: methanol (V / V)=1:1 , collecting part of the eluate from undergrowth ginseng saponin B, recovering the solvent to obtain the thick product of undergrowth ginseng saponin B, with a yield of 0.016%;
[0039] c. Purification of undergrowth ginsenoside B: the separated crude undergrowth ginsenoside B was purified by preparative liquid chr...
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