Enzyme conjugate stabilizing solution

A technology of enzyme conjugates and stabilizing solutions, which is applied in the direction of enzyme stabilization, can solve the problems of easy loss of activity, etc., and achieve the effect of non-toxic and harmless to the human body, simple operation, and easy preparation

Active Publication Date: 2011-05-11
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In view of this, the present invention proposes an enzyme conjugate stabilizing solution to solve the problem tha

Method used

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Examples

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Example Embodiment

[0019] Example 1

[0020] The TSH chemiluminescence immunoassay method established by preparing an anti-thyroid stimulating hormone (TSH) monoclonal antibody-horseradish peroxidase (HRP) conjugate working solution according to the embodiment of the present invention includes the following steps:

[0021] Step 1. Prepare the enzyme conjugate stabilizing solution, the components and concentrations are:

[0022]

[0023]

[0024] Step 2. Coat the white light-emitting plate with anti-TSH monoclonal antibody at a concentration of 5ug / ml, 100ul / well, and coat at 37°C for 2 hours. After washing the plate, coat with blocking solution at 200 / well at 4°C for 18 hours Then, discard the sealing solution in the coating plate, pat dry, and place it in a drying oven at 37°C with a humidity of 25% for 4 hours;

[0025] Step 3. Dilute the anti-TSH monoclonal antibody-HRP enzyme conjugate with the enzyme conjugate stabilizing solution to a concentration of 1ug / ml, and then place the prepared working so...

Example Embodiment

[0031] Example 2

[0032] The TSH chemiluminescence immunoassay method established by preparing an anti-thyroid stimulating hormone (TSH) monoclonal antibody-horseradish peroxidase (HRP) conjugate working solution according to the embodiment of the present invention includes the following steps:

[0033] Step 1. Prepare the enzyme conjugate stabilizing solution, the components and concentrations are:

[0034]

[0035] Step 2. Coat the white light-emitting plate with anti-TSH monoclonal antibody at a concentration of 5ug / ml, 100ul / well, and coat at 37°C for 2 hours. After washing the plate, coat with blocking solution at 200 / well at 4°C for 18 hours Then, discard the sealing solution in the coating plate, pat dry, and place it in a drying oven at 37°C with a humidity of 25% for 4 hours;

[0036] Step 3. Dilute the anti-TSH monoclonal antibody-HRP enzyme conjugate with the enzyme conjugate stabilizing solution to a concentration of 1ug / ml, and then place the prepared enzyme conjugate wo...

Example Embodiment

[0042] Example 3

[0043] The TSH chemiluminescence immunoassay method established by preparing an anti-thyroid stimulating hormone (TSH) monoclonal antibody-horseradish peroxidase (HRP) conjugate working solution according to the embodiment of the present invention includes the following steps:

[0044] Step 1. Prepare the enzyme conjugate stabilizing solution, the components and concentrations are:

[0045]

[0046] Step 2. Coat the white light-emitting plate with anti-TSH monoclonal antibody at a concentration of 5ug / ml, 100ul / well, and coat at 37°C for 2 hours. After washing the plate, coat with blocking solution at 200 / well at 4°C for 18 hours Then, discard the sealing solution in the coating plate, pat dry, and place it in a drying oven at 37°C with a humidity of 25% for 4 hours;

[0047] Step 3. Dilute the anti-TSH monoclonal antibody-HRP enzyme conjugate with the enzyme conjugate stabilizing solution to a concentration of 1ug / ml, and then place the prepared enzyme conjugate wo...

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Abstract

The invention provides an enzyme conjugate stabilizing solution which comprises the following components: 1-7.5g/L protein material, 5-50g/L saccharide material, 2-30g/L amino acid material, 5-50g/L antipyrine, 1-10 g/L vitamin C, 0.2-5g/L surfactant, 0.5-5 million IU/L gentamycin sulfate, 0.5g/L Proclin 300 and 0.02mol/L phosphate buffer solution the pH value of which is 7.4. By using the enzyme conjugate stabilizing solution provided by the invention, after the enzyme conjugate is not in a low temperature environment, the bioactivity of the enzyme conjugate can be maintained for a longer time, thereby solving the problem that the enzyme conjugate is easy to lose activity after the enzyme is not in the low temperature storage environment in the related technology. In addition, the enzyme conjugate stabilizing solution provided by the invention is easy to prepare, the sensitivity is not affected, the cost is low, the operation is simple, environmental pollution can not be caused, the stabilizing solution is nontoxic and harmless to the human body and the like.

Description

technical field [0001] The invention relates to an enzyme conjugate stabilizing solution. Background technique [0002] At present, the most widely used immunoassay methods in the clinical field are enzyme-linked diagnostic reagents (EIA) and chemiluminescent immunoassays (CLIA). Among them, since the conjugates of trace antigens or antibodies and enzymes (HRP or AP) are easily inactivated after being formulated into ordinary buffers (such as PBS), both EIA and CLIA methods involve a key technology, that is, maintaining Stability of Enzyme Conjugates. [0003] The commonly used enzyme conjugate diluents cannot effectively maintain the activity of trace antigen or antibody and enzyme (HRP or AP) conjugates. The method to maintain the biological activity of the enzyme conjugate is usually to store the enzyme conjugate working solution in an environment below -20°C, and if it is stored at 2-8°C for one week or destroyed for one day at 37°C, its biological activity will be los...

Claims

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Application Information

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IPC IPC(8): C12N9/96
Inventor 刘萍栾大伟
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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