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Enzyme conjugate stabilizing solution

A technology of enzyme conjugates and stabilizing solutions, which is applied in the direction of enzyme stabilization, can solve the problems of easy loss of activity, etc., and achieve the effect of non-toxic and harmless to the human body, simple operation, and easy preparation

Active Publication Date: 2011-05-11
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention proposes an enzyme conjugate stabilizing solution to solve the problem that the enzyme conjugate in the related art is prone to lose activity after leaving the low-temperature storage environment

Method used

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Examples

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Embodiment 1

[0020] The TSH chemiluminescent immunoassay method established with the embodiment of the present invention to prepare anti-thyroid-stimulating hormone (TSH) monoclonal antibody-horseradish peroxidase (HRP) conjugate working solution includes the following steps:

[0021] Step 1, preparing enzyme conjugate stable solution, its component and concentration are:

[0022]

[0023]

[0024] Step 2. Coat the white light-emitting plate with anti-TSH monoclonal antibody, the coating concentration is 5ug / ml, 100ul / well, and coat at 37°C for 2 hours. After washing the plate, coat with blocking solution at 200 / well for 18 hours at 4°C Finally, discard the blocking solution in the coated plate, pat it dry, and dry it in a 37°C drying oven with a humidity of 25% for 4 hours;

[0025] Step 3. Dilute the anti-TSH monoclonal antibody-HRP enzyme conjugate with the enzyme conjugate stabilizing solution to a concentration of 1ug / ml, and then place the prepared enzyme conjugate working solu...

Embodiment 2

[0032] The TSH chemiluminescent immunoassay method established with the embodiment of the present invention to prepare anti-thyroid-stimulating hormone (TSH) monoclonal antibody-horseradish peroxidase (HRP) conjugate working solution includes the following steps:

[0033] Step 1, preparing enzyme conjugate stable solution, its component and concentration are:

[0034]

[0035] Step 2. Coat the white light-emitting plate with anti-TSH monoclonal antibody, the coating concentration is 5ug / ml, 100ul / well, and coat at 37°C for 2 hours. After washing the plate, coat with blocking solution at 200 / well for 18 hours at 4°C Finally, discard the blocking solution in the coated plate, pat it dry, and dry it in a 37°C drying oven with a humidity of 25% for 4 hours;

[0036] Step 3. Dilute the anti-TSH monoclonal antibody-HRP enzyme conjugate with the enzyme conjugate stabilizing solution to a concentration of 1ug / ml, and then place the prepared enzyme conjugate working solution in a th...

Embodiment 3

[0043] The TSH chemiluminescent immunoassay method established with the embodiment of the present invention to prepare anti-thyroid-stimulating hormone (TSH) monoclonal antibody-horseradish peroxidase (HRP) conjugate working solution includes the following steps:

[0044] Step 1, preparing enzyme conjugate stable solution, its component and concentration are:

[0045]

[0046] Step 2. Coat the white light-emitting plate with anti-TSH monoclonal antibody, the coating concentration is 5ug / ml, 100ul / well, and coat at 37°C for 2 hours. After washing the plate, coat with blocking solution at 200 / well for 18 hours at 4°C Finally, discard the blocking solution in the coated plate, pat it dry, and dry it in a 37°C drying oven with a humidity of 25% for 4 hours;

[0047] Step 3. Dilute the anti-TSH monoclonal antibody-HRP enzyme conjugate with the enzyme conjugate stabilizing solution to a concentration of 1ug / ml, and then place the prepared enzyme conjugate working solution in a th...

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Abstract

The invention provides an enzyme conjugate stabilizing solution which comprises the following components: 1-7.5g / L protein material, 5-50g / L saccharide material, 2-30g / L amino acid material, 5-50g / L antipyrine, 1-10 g / L vitamin C, 0.2-5g / L surfactant, 0.5-5 million IU / L gentamycin sulfate, 0.5g / L Proclin 300 and 0.02mol / L phosphate buffer solution the pH value of which is 7.4. By using the enzyme conjugate stabilizing solution provided by the invention, after the enzyme conjugate is not in a low temperature environment, the bioactivity of the enzyme conjugate can be maintained for a longer time, thereby solving the problem that the enzyme conjugate is easy to lose activity after the enzyme is not in the low temperature storage environment in the related technology. In addition, the enzyme conjugate stabilizing solution provided by the invention is easy to prepare, the sensitivity is not affected, the cost is low, the operation is simple, environmental pollution can not be caused, the stabilizing solution is nontoxic and harmless to the human body and the like.

Description

technical field [0001] The invention relates to an enzyme conjugate stabilizing solution. Background technique [0002] At present, the most widely used immunoassay methods in the clinical field are enzyme-linked diagnostic reagents (EIA) and chemiluminescent immunoassays (CLIA). Among them, since the conjugates of trace antigens or antibodies and enzymes (HRP or AP) are easily inactivated after being formulated into ordinary buffers (such as PBS), both EIA and CLIA methods involve a key technology, that is, maintaining Stability of Enzyme Conjugates. [0003] The commonly used enzyme conjugate diluents cannot effectively maintain the activity of trace antigen or antibody and enzyme (HRP or AP) conjugates. The method to maintain the biological activity of the enzyme conjugate is usually to store the enzyme conjugate working solution in an environment below -20°C, and if it is stored at 2-8°C for one week or destroyed for one day at 37°C, its biological activity will be los...

Claims

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Application Information

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IPC IPC(8): C12N9/96
Inventor 刘萍栾大伟
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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