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Composite microecological preparation capable of degrading nitrites in water body rapidly

A technology of compound microecology and nitrite, applied in the field of compound microecology preparations

Inactive Publication Date: 2011-05-18
TIANJIN CITY AGRI BIO TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Lactic acid bacteria have been used more and more as feed additives listed by the Ministry of Agriculture, and their effects on degrading nitrite in water have also been reported.

Method used

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  • Composite microecological preparation capable of degrading nitrites in water body rapidly
  • Composite microecological preparation capable of degrading nitrites in water body rapidly
  • Composite microecological preparation capable of degrading nitrites in water body rapidly

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] The water purification function screening of embodiment 1 single strain bacterium

[0013] Degradation Effect of Denitrifying Bacteria on Nitrite

[0014] Fill the glass container with 2.5L simulated aquaculture wastewater, and adjust the initial concentration of nitrite nitrogen to 35mg L -1 , put 50mL of bacterial solution into it and place it in a 30°C incubator for cultivation. Taking no bacteria as a control, samples were taken regularly to measure the content of nitrite in the water. The result is as figure 1 .

[0015] Depend on figure 1 It can be seen that when denitrifying bacteria are introduced into the water body, the concentration of nitrite is 32 mg L within 24 hours. -1 The degradation rate of the simulated wastewater was 60.9%, and the nitrite was completely degraded within 40 hours, and the degradation effect was significantly better than that of the control.

[0016] Degradation Effect of Lactic Acid Bacteria on Nitrite

[0017] Fill the glass co...

Embodiment 2

[0022] The water quality purification effect (29 ℃) of embodiment 2 compound probiotics

[0023] Configure simulated aquaculture water: place the water in the aquaculture pond indoors for 2 days after being sand-filtered, mix it with tap water 1:1, and measure the initial nitrate, nitrite content and pH value in the mixed water. Add an appropriate amount of nitrite to make the nitrite nitrogen in the water 12mg L -1 . 3L of water per tank.

[0024] Inoculation method: Centrifuge 10mL of Bacillus amyloliquefaciens and 10mL of lactic acid bacteria solution respectively (7500rpm for 10min), remove the supernatant, add the sludge into the water body, and directly add 10ml of denitrifying bacteria. With no bacteria as the control, each treatment was replicated 3 times.

[0025] Use incandescent lamps to increase light for 7-8 hours during the day, and the water temperature is about 29°C. Each water sample was taken, centrifuged at 7500rpm, and the supernatant was taken for dete...

Embodiment 3

[0027] The water quality purification effect (25 ℃) of embodiment 3 composite probiotics

[0028] Test method is the same as embodiment 2. The temperature of the water body is about 25°C.

[0029] Depend on Figure 7 It can be seen that the degradation rate of nitrite reached 81.8% within 24 hours and 99.9% within 48 hours of inoculation treatment at 25°C compared with the control. Depend on Figure 8 It can be seen that the degradation rate of nitrate reached 82.7% within 24 hours, and 97.0% within 48 hours. The degradation effect is lower than that at 29°C. Depend on Figure 9 It can be seen that the inoculation treatment can significantly reduce the pH value of the water body compared with the control.

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Abstract

The invention discloses a composite microecological preparation capable of degrading nitrites in a water body rapidly. The composite microecological preparation is prepared from denitrifying bacteria, the lactic acid bacteria and the bacillus amyloliquefaciens are matched according to the ratio of 1:1:1 by taking denitrifying bacteria, lactic acid bacteria and bacillus amyloliquefaciens as active ingredients. The composite microecological preparation is characterized in that in the water body polluted with the nitrites, the composite microecological preparation with certain concentration is put, and the nitrites in the water body can be degraded rapidly in 24 h, therefore, the environment of the water body is improved, and the hazard of the nitrites to creatures in the water body is reduced.

Description

technical field [0001] The invention relates to a composite micro-ecological preparation capable of rapidly degrading nitrite in water, belonging to the technical field of biological bacteria preparation and the field of environmental microorganism application. Background technique [0002] Nitrite mainly refers to sodium nitrite and potassium nitrite. Because it can convert hemoglobin in animal red blood cells into methemoglobin, the blood cells lose the ability to transport oxygen, resulting in death of animals due to hypoxia. It is a highly toxic substance. [0003] In recent years, due to the application of chemical fertilizers, sewage irrigation, garbage manure, industrial nitrogenous waste, and nitrogenous waste discharged from fuel combustion in the environment, under natural conditions, nitrite is formed after precipitation leaching and decomposition, which flows into rivers and lakes and infiltrates into Underground, resulting in nitrite pollution of groundwater and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34
Inventor 张峰峰赵玉洁谢风行周可李亚玲
Owner TIANJIN CITY AGRI BIO TECH RES CENT