Nanometer vaccine and preparation method thereof

A technology of nano-vaccine and vaccine carrier, which is applied in the field of vaccine and its preparation, can solve the problems of reducing cytotoxicity, etc., and achieve the effect of reducing cytotoxicity, powerful immune adjuvant effect, and improving ability

Inactive Publication Date: 2011-05-25
SHENZHEN INST OF ADVANCED TECH
View PDF1 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of this, it is necessary to address the defects of existing liposome vaccine carriers, and propose a new mannosylated cationic liposome complex, which can be used as a vaccine carrier and applied to vaccines. This new liposome The complex incorporates neutral phospholipids and mannose in cationic

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nanometer vaccine and preparation method thereof
  • Nanometer vaccine and preparation method thereof
  • Nanometer vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0048] Example 1: Mannosylated cationic liposome complex

[0049] The present invention proposes to use a mannosylated cationic liposome complex as a novel vaccine carrier to construct a safe and efficient nano vaccine.

[0050] For the structure of mannosylated cationic liposome complex, see figure 1 , Including the cationic lipid, neutral phospholipid and mannoside mixed in proportion, the molar ratio of cationic lipid and neutral phospholipid is 19:1 to 2:18, the molar ratio of mannoside and liposome including cationic lipid and neutral phospholipid The ratio is 2:98 to 20:80.

[0051] Cationic lipids are positively charged amphiphilic molecules, including a positively charged head and a hydrophobic tail. The positively charged polar head contains amino groups, quaternary ammonium salts and polyamines and other amine groups, while the hydrophobic tail includes saturated Or unsaturated fatty acid chain or cholesterol ring, the main chain of the molecule is glycerol, the second hyd...

Example Embodiment

[0054] Example 2: Vaccine carrier with immune adjuvant effect

[0055] The vaccine carrier with immune adjuvant function of the present invention encapsulates vaccine antigen, and the vaccine carrier is a mannosylated cationic liposome complex.

[0056] For the structure of mannosylated cationic liposome complex, see figure 1 , Including the cationic lipid, neutral phospholipid and mannoside mixed in proportion, the molar ratio of cationic lipid and neutral phospholipid is 19:1 to 2:18, the molar ratio of mannoside and liposome including cationic lipid and neutral phospholipid The ratio is 2:98 to 20:80. Cationic lipids are positively charged amphiphilic molecules, including a positively charged head and a hydrophobic tail. The positively charged polar head contains amino groups, quaternary ammonium salts and polyamines and other amine groups, while the hydrophobic tail includes saturated Or unsaturated fatty acid chain or cholesterol ring. It is an amphiphilic molecule with posit...

Example Embodiment

[0057] Example 3: Nano vaccine

[0058] The nano-vaccine of the present invention includes vaccine antigens and liposomes encapsulating the vaccine antigens. The liposomes are mannosylated cationic liposome complexes. The antigens are various proteins, polypeptides, polysaccharides, DNA or RNA, and the antigens are derived from For viruses, bacteria, other microorganisms, tumors or genetically engineered protein products.

[0059] For the structure of mannosylated cationic liposome complex, see figure 1 , Including the cationic lipid, neutral phospholipid and mannoside mixed in proportion, the molar ratio of cationic lipid and neutral phospholipid is 19:1 to 2:18, the molar ratio of mannoside and liposome including cationic lipid and neutral phospholipid The ratio is 2:98 to 20:80. Cationic lipids are positively charged amphiphilic molecules, including a positively charged head and a hydrophobic tail. The positively charged polar head contains amino groups, quaternary ammonium sal...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a nanometer vaccine and a preparation method thereof. A new mannose glycosylated-cationic liposome complex can be used as a vaccine vector and can be applied to the vaccine. In the new liposome complex, neutral phospholipid and mannose are mixed into cationic lipid, so the immunologic adjuvant effect and the antigen-presenting cell targeting property of a liposome are improved, and the cytotoxic effect of the liposome is reduced obviously. The invention provides a novel high-efficiency vaccine vector and an adjuvant which do not have side effects and can improve the immunologic effect of the vaccine obviously.

Description

【Technical field】 [0001] The invention relates to a vaccine and a preparation method thereof, in particular to a vaccine using liposome as a carrier and a preparation method thereof. [0002] The present invention also relates to a vaccine carrier. [0003] The invention also relates to a liposome complex applicable to vaccine carriers. 【Background technique】 [0004] Vaccines are an important means to prevent and control the occurrence and development of infectious diseases. Immune adjuvants (also known as non-specific immune proliferators) are indispensable components in vaccines, which can effectively promote specific immune responses induced by vaccines. With the rapid development of vaccine research, new genetically engineered vaccines are being widely used in the research and development of vaccines. This vaccine has the advantages of high purity and strong specificity, but due to its small molecule and relatively poor immunogenicity, it is urgent to combine effecti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A61K47/26A61K9/127A61K39/39A61P31/00
Inventor 马轶凡周冬梅谢晓芳庄燕郑明彬
Owner SHENZHEN INST OF ADVANCED TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap