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Marsdenia tenacissima saponins H and preparation method and application thereof

A technology of black bone vine and saponin, which is applied in the field of medicine, can solve problems such as difficult separation and purification, failure to meet the requirements of clear structure of active ingredients and controllable quality, etc.

Inactive Publication Date: 2011-05-25
JIANGSU TIANSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Although it has been clarified that the main active ingredients in Aegypti japonica are a large class of saponins, since there are many types of acacia saponins, and many of them have similar structures, it is very difficult to separate and purify, and it is difficult to further clarify the true activity of this type of saponins. It is difficult to effectively control the quality of this product, and it cannot meet the national requirements for the active ingredients of modern traditional Chinese medicine with clear structure and controllable quality, which seriously restricts the further development and improvement of this product.

Method used

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  • Marsdenia tenacissima saponins H and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Take 20kg of the medicinal material of Aegypti sinensis, add 140L of 80% ethanol to extract under reflux for 3 times, each time for 1.5 hours, and combine the extract; After the column is completed, add water to elute to remove the large polar impurities, and then use 80% ethanol to elute until there is no black bone saponin H in the eluent. Drying under reduced pressure, crushing into 80 mesh powder, to obtain 440g of crude product of Aegypti japonica total saponins;

[0043] Add 2.2L of ethyl acetate to the above-mentioned crude total saponins of Acacia japonica, heat and reflux for extraction for 3 times, filter, discard the residue, combine and concentrate the extracts to 2.0L, cool, add a small amount of acacia saponin H with a purity of 99.1% The crystal was used as a seed crystal, and it was left to stand for 12 hours to crystallize, filtered, and dried to obtain 106 g of off-white crude Aeginaside H.

[0044] The above-mentioned crude product of ebonyside H was...

Embodiment 2

[0046] Take 50kg of Chinese herbal medicine, add 250L of 95% ethanol to heat and reflux for extraction 4 times, each time for 1 hour, and combine the extract; the extract is concentrated to 250L, filtered, and the filtrate is put on the D-101 macroporous adsorption resin column, and the column is completed Finally, add water to elute to remove the large polar impurities, then use 200L 95% ethanol to elute until there is no black bone saponin H in the eluent, collect the eluate, concentrate under reduced pressure at 65°C to a thick paste, and store the thick paste in Dried under reduced pressure at 65°C, crushed into 60-mesh powder, and obtained 1.4kg of crude product of total saponins of Aegypti japonica;

[0047] Add 14L of isopropyl ether to the above-mentioned crude total saponins of Aegia saponins, heat and reflux to extract twice, filter, discard the residue, combine and concentrate the extracts to 7.0L, cool, add a trace amount of crystals of Aegia saponin H with a purity...

Embodiment 3

[0050] Take 50kg of Aegypti sinensis medicinal material, add 500L of water, heat and reflux for extraction 3 times, each time for 2 hours, and combine the extract; After removing the large polar impurities by elution, use 300L 50% ethanol for elution, until there is no black bone saponin H in the eluent, collect the eluate, concentrate under reduced pressure at 75°C to a thick paste, and reduce the thick paste at 75°C. Compressed and dried, crushed into 100 mesh powder to obtain 1.6kg of crude product of Aegypti saponins;

[0051] Add 13L of isopropyl ether-ethyl acetate (1:1) to the above-mentioned crude total saponins of Acacia japonica, heat and reflux for extraction 3 times, filter, discard the residue, combine and concentrate the extracts to 6.5L, cool, add a trace amount of 99.3% of the keloidin H crystals were used as seed crystals, left to crystallize for 18 hours, filtered, and dried to obtain 252 g of white-like keloidin H crude product.

[0052] The above-mentioned...

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Abstract

The invention provides a Marsdenia tenacissima saponins H and a preparation method thereof. According to the preparation method, traditional Chinese medicine Marsdenia tenacissima is used as raw material, Marsdenia tenacissima saponins H with good antineoplastic activity and high content is obtained through a series of steps such as extraction, separating, crystallization, recrystallization and the like, and a high efficiency liquid phase chromatography is adopted to determine that the content of the marsdenia tenacissima saponins H is in a range of 60-99.8%. Proper pharmaceutically acceptable carriers can be added to the Marsdenia tenacissima saponins H to prepare certain dosage form, so as to be used for clinic treatment of tumour. The Marsdenia tenacissima saponins H and the preparation method thereof of the invention employ traditional Chinese medicine modernization separating and purifying technology, the technology is innovative, is suitable for industrialization production, and has strong practicality.

Description

technical field [0001] The invention relates to a preparation method and application of akabasaponin H with antitumor activity, belonging to the field of medicine. Background technique [0002] Tumor is the number one killer of human life identified by the World Health Organization (WHO), and has become one of the major diseases that are common and seriously threaten human life and quality of life. More and more serious threats to human health and life safety. [0003] Our country has a long history of using Chinese herbal medicine to treat various diseases. Screening new anti-cancer drugs from Chinese herbal medicine has its own advantages. Many of the anti-tumor drugs that have been marketed are made from active ingredients extracted from natural Chinese herbal medicine. It plays a very important role in the treatment of tumors. Therefore, using advanced separation and purification technology to develop anti-tumor drugs with good activity from natural products is not only...

Claims

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Application Information

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IPC IPC(8): C07J71/00A61K31/7048A61P35/00
Inventor 季浩杨永安陈月红
Owner JIANGSU TIANSHENG PHARMA
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