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Beauveria bassiana and application thereof in prevention and treatment of tobacco brown spot

A technology for Beauveria bassiana and tobacco red spot, which is applied in the fields of application, fungicides, fungi, etc., to achieve obvious inhibitory effect and good inhibition rate

Active Publication Date: 2019-01-04
ANHUI AGRICULTURAL UNIVERSITY +7
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problem that there is no effective use of Beauveria bassiana to suppress the existing tobacco red spot disease, the present invention provides a Beauveria bassiana and its application in the prevention and treatment of tobacco red spot disease, which can effectively inhibit tobacco red spot disease

Method used

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  • Beauveria bassiana and application thereof in prevention and treatment of tobacco brown spot
  • Beauveria bassiana and application thereof in prevention and treatment of tobacco brown spot
  • Beauveria bassiana and application thereof in prevention and treatment of tobacco brown spot

Examples

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Embodiment 1

[0031] Plate Confrontation Experiment of Beauveria bassiana against Tobacco Alternative Spot Disease

[0032] Two strains of the tested plant pathogenic fungus Alternaria tabacum Alternaria and Beauveria bassiana (numbered HFBb009 and DSXJ-07) were transferred to the SDAY medium by the plate method, and placed in an artificial climate box at 25°C±1°C. After inverting for 7 days under dark conditions (for specific applications, values ​​such as 8d, 9d, 10d, 11d, 12d, 13d, 14d, 15d, etc. can also be selected), use a hole puncher with a diameter of 6mm to punch the bacteria plate on the edge of each colony , using the flat-plate confrontation culture method, inoculate Alternaria alternifolia in the center of the SD AY plate, and inoculate the strain of Beauveria bassiana for testing on the edge of the SDAY plate, with a distance of about 3.0 cm between the two. The Alternaria flat plate was used as the control, and each treatment was repeated 3 times, and placed in an artificial ...

Embodiment 2

[0042] Plate inhibitory assay of different concentrations of Beauveria bassiana spore suspension on tobacco red spot

[0043] Two strains of Beauveria bassiana (numbers HFBb009 and DSXJ-07) were inoculated into SDAY liquid medium respectively, cultured in a constant temperature culture shaker for 3 days, and 0.1ml of the culture was drawn and plated on SDAY solid medium for 15 days. , pick a certain amount of mycelia and wash the spores with 0.05% Tween 80 aqueous solution in a 10ml test tube, filter the mycelium with 4 layers of lens-cleaning paper, and then use the serial dilution method to dilute the spores to 1.0×10 8 spores / mL, 1.0×10 7 spores / mL, 1.0×10 6 spores / mL, 1.0×10 5 spores / mL, 1.0×10 4 spores / mL and 1.0×10 3 The spore suspension with a concentration of 3 spores / mL was applied to the plates respectively, and the tobacco Alternaria disc (6mm) was inoculated in the center of the plate, and each treatment was repeated 3 times, and then only the tobacco was inocu...

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Abstract

The invention discloses Beauveria bassiana and application thereof in the prevention and treatment of tobacco brown spot and belongs to the technical field of plant protection. Beauveria bassiana is numbered HFBb009 and collected in China General Microbiological Culture Collection Center under 14158. Beauveria bassiana is used herein for the first time in the prevention and treatment of tobacco brown spot; data shows that Beauveria bassiana (numbered HFBb009) can well inhibit tobacco brown spot; the inhibitory action of Beauveria bassiana upon the pathogenic fungus tobacco brown spot pathogengrows stronger overtime, and the inhibitory action is most significant when Beauveria bassiana spore suspension having the concentration of 1.0*107 is used.

Description

technical field [0001] The invention belongs to the technical field of plant protection, and in particular relates to a Beauveria bassiana and its application in the prevention and treatment of tobacco red spot disease. Background technique [0002] Tobacco red spot disease is a very common and widely distributed fungal disease worldwide, which seriously affects the production of tobacco and causes economic losses of hundreds of millions of dollars. [0003] After 1980, the occurrence of red spot disease in various tobacco areas in China increased sharply every day, and the incidence rate of leaves was as high as 80%, which reduced the quality of tobacco production by two grades on average. The production reduction in severe disease areas is as high as more than 50%, which directly affects the quantity of high-quality cigarettes produced. Red spot disease mainly damages leaves. It was dark yellow dots at first, and then gradually expanded into circles or irregular lesions ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
CPCC12N1/14A01N63/30C12R2001/645C12N1/145
Inventor 李茂业刘明科李斌陈德鑫张继刚冯长春顾勇刘东阳张瑞平闫芳芳顾会战徐传涛江连强杨洋杨懿德
Owner ANHUI AGRICULTURAL UNIVERSITY
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