Method for improving biomass of lactic acid bacteria at low pH by adding glutamic acid

A technology of glutamic acid and lactic acid bacteria, applied in the direction of microorganism-based methods, biochemical equipment and methods, and adding compounds to stimulate growth, etc., can solve the problems of harmful effects on cell physiology, damage, reduction of protein and DNA, etc., and achieve improvement Anti-acid performance of the bacteria, good growth performance, and low professional quality requirements

Inactive Publication Date: 2011-05-25
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Internal acidifying conditions also reduce the activity of acid-sensitive enzymes and cause permanent damage to proteins and DNA, accompanied by a large accumulation of anions generated by the dissociation process, which can also have deleterious effects on cell physiology

Method used

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  • Method for improving biomass of lactic acid bacteria at low pH by adding glutamic acid
  • Method for improving biomass of lactic acid bacteria at low pH by adding glutamic acid
  • Method for improving biomass of lactic acid bacteria at low pH by adding glutamic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1 glutamic acid addition 2 g / L

[0017] Table 1 Determination of bacterial biomass and final pH

[0018]

[0019] When 2 g / L glutamic acid was added, the final cell concentration of Lactobacillus casei CCTCC M 2010163 increased by 5% compared with the control group (without addition); the final pH also increased to 4.4, and the acid resistance ability was improved.

Embodiment 2

[0020] Embodiment 2 glutamic acid addition 5 g / L

[0021] Table 2 Determination of bacterial biomass and termination pH

[0022]

[0023] When 5 g / L of glutamic acid was added, the final cell concentration of Lactobacillus casei CCTCC M 2010163 increased by 15% compared with the control group (without addition); the final pH was also increased to 4.6, shortening the logarithmic growth phase of fermentation for 1 h, and acid-resistant The ability is significantly improved.

Embodiment 3

[0024] Embodiment 3 glutamic acid addition amount 10 g / L

[0025]

[0026] When 10 g / L of glutamic acid was added, the final cell concentration of Lactobacillus casei CCTCC M 2010163 was increased by 9% compared with the control group (without addition); the final pH was also increased to 4.5, and the acid resistance ability was improved.

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Abstract

The invention discloses a method for improving biomass of lactic acid bacteria at low pH by adding glutamic acid and application thereof which belong to the technical field of bioengineering. By using the method of adding the glutamic acid into a culture medium, the biomass of lactobacillus casei CCTCCM2010163 under an extreme pH condition is improved. The method is simple and is easy to operate, and can be used for fermentation production of the lactic acid bacteria.

Description

technical field [0001] The invention relates to a method for increasing the biomass of lactic acid bacteria under low pH conditions by adding glutamic acid, and belongs to the technical field of fermentation engineering. Background technique [0002] The growth characteristic of lactic acid bacteria is to produce acid as the final fermentation product, and the lactic acid produced is released and accumulated in the extracellular environment. Organic acids can inhibit the growth of other microorganisms in the natural environment, but they also greatly affect the growth of other microorganisms in high-density fermentation. Its own activity is reduced; in addition, lactic acid bacteria encounter a stronger inorganic acid environment in the stomach after eating and digesting, and the pH of normal gastric juice in the human body is often lower than 3.0, which is consistent with the optimal growth of most lactic acid bacteria 5-9 The pH is very different. The combination of the t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12R1/245
Inventor 陈坚顾磊张娟堵国成
Owner JIANGNAN UNIV
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