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Fingerprint test method for adhesive rehmannia leaf total glycoside

A measurement method, fingerprint technology, applied in the field of spectrum measurement, can solve problems affecting the clinical efficacy of drugs, achieve product quality and efficacy assurance, reduce human processing, and reduce the effect of possibility

Inactive Publication Date: 2011-05-25
SICHUAN MEDCO PHARML
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the prior art, only the single component of verbascoside is used for the control determination to measure the content of total glucosides of rehmannia glucoside, which cannot fully reflect the real situation of its medicinal effect components and affect the clinical efficacy of its medicine.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Determination method of local glucosides fingerprint

[0019] Chromatographic conditions and system suitability test Use octadecylsilane bonded silica gel as filler; methanol: glacial acetic acid: water = 30:0.5:62 as mobile phase, flow rate 1ml / min, detection wavelength 330nm, column temperature 35°C, theoretical plate number according to ergosteroside peak Calculate, it should not be less than 8500.

[0020] Preparation of reference solution Take 8mg of acteoside reference substance, weigh it accurately, put it in a 50ml measuring bottle, add methanol: glacial acetic acid: water = 30:0.5:62 mixed solvent to dissolve and dilute to the scale, shake well, that is, every 1ml contains ergosteroside 0.16 mg.

[0021] Preparation of the test solution Take 60mg of the fine powder of this product, weigh it accurately, put it in a 50ml measuring bottle, add 45ml of methanol: glacial acetic acid: water = 30:0.5:62 mixed solution, ultrasonicate for 20 minutes, let ...

Embodiment 2

[0024] Determination method of local glucosides fingerprint

[0025] Chromatographic conditions and system suitability test Octadecylsilane bonded silica gel is used as filler; methanol: glacial acetic acid: water = 33:1:66 as mobile phase, flow rate is 1ml / min, detection wavelength is 330nm, column temperature is 35°C, and the number of theoretical plates is based on ergosteroside peak Calculate, it should not be less than 8500.

[0026] Preparation of reference solution Take 8 mg of verbascoside reference substance, weigh it accurately, put it in a 50ml measuring bottle, add methanol: glacial acetic acid: water = 33:1:66 mixed solvent to dissolve and dilute to the scale, shake well, that is, every 1ml contains ergosteroside 0.16 mg.

[0027] Preparation of the test solution Take 60mg of the fine powder of this product, weigh it accurately, put it in a 50ml measuring bottle, add 45ml of methanol: glacial acetic acid: water = 33:1:66 mixed solution, sonicate for...

Embodiment 3

[0030] Determination method of local glucosides fingerprint

[0031] Chromatographic conditions and system suitability test Use octadecylsilane bonded silica gel as filler; methanol: glacial acetic acid: water = 36: 1.5: 70 as mobile phase, flow rate 1ml / min, detection wavelength 330nm, column temperature 35°C, theoretical plate number according to ergosteroside peak Calculate, it should not be less than 8500.

[0032] Preparation of reference solution Take 8 mg of verbascoside reference substance, weigh it accurately, put it in a 50ml measuring bottle, add methanol: glacial acetic acid: water = 36:1.5:70 mixed solvent to dissolve and dilute to the scale, shake well, that is, every 1ml contains ergosteroside 0.16 mg.

[0033] Preparation of the test solution Take 60mg of the fine powder of this product, weigh it accurately, put it in a 50ml measuring bottle, add 45ml of methanol: glacial acetic acid: water = 36:1.5:70 mixed solution, ultrasonicate for 20 minutes...

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PUM

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Abstract

The invention discloses a fingerprint test method for adhesive rehmannia leaf total glycoside. By the method, the quality situation of the adhesive rehmannia leaf total glycoside can be grasped from the aspect of the overall characteristic of a liquid chromatogram, and the composition specificity of the adhesive rehmannia leaf total glycoside can be represented better through retention time and fingerprint peak characteristics. A high performance liquid chromatography (HPLC) fingerprint of the adhesive rehmannia leaf total glycoside which is constructed by the method can be used for detecting and evaluating the product quality of the adhesive rehmannia leaf total glycoside. In the method, the HPLC fingerprint of the adhesive rehmannia leaf total glycoside is taken as a quality control measure, so that quality one-sidedness caused by the judgment made by taking a single chemical composition as a comparison reference is avoided, and the possibility of manual treatment for ensuring quality conformance is lowered. By the test method, an effective technical measure for complete and correct evaluation of the quality of the adhesive rehmannia leaf total glycoside is provided, and the quality and curative effect of the product are further ensured.

Description

technical field [0001] The invention relates to a spectrum determination method, in particular to a fingerprint spectrum determination method of rehmannia glucosides. Background technique [0002] Rehmannia glucosides is a drug extracted from rehmannia rehmannia leaves for the treatment of chronic glomerulonephritis. More than 50% of the main active ingredients in rehmannia rehmannia glucosides are acteoside (acteoside), cisacteoside, isoacteoside, hydroxyacteoside, methoxyacteoside, jionosideA1, jionosideB1 and other phenylethanol glycosides. Among them, verbascoside, the main component, accounts for more than 25% of the total glucosides of Rehmannia glutinosa. However, in the prior art, only verbascoside is used as a control to measure the content of total glycosides of rehmannia glucoside, which cannot fully reflect the real situation of its medicinal components and affect the clinical efficacy of its medicine. Contents of the invention [0003] The purpose of the pre...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 张孝齐边宝林杨建
Owner SICHUAN MEDCO PHARML