Method for efficiently separating and detecting theanine enantiomer by capillary electrophoresis

A technology of capillary electrophoresis and theanine is applied in the direction of material analysis, measurement device, and analysis material by electromagnetic means, and achieves the effects of low cost, good separation effect and short analysis time.

Inactive Publication Date: 2011-06-15
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the separation and detection of theanine enantiomers using capillary electrophoresis technology in domestic and foreign literature. The present invention uses this technology to establish an analytical method with good separation effect and high sensitivity, which is applied to the analysis and detection of theanine enantiomers.

Method used

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  • Method for efficiently separating and detecting theanine enantiomer by capillary electrophoresis
  • Method for efficiently separating and detecting theanine enantiomer by capillary electrophoresis
  • Method for efficiently separating and detecting theanine enantiomer by capillary electrophoresis

Examples

Experimental program
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Effect test

Embodiment 1

[0017] Borate solution with a pH value of 11 was used as the buffer solution, and 20 mmol / L β-cyclodextrin was added as the chiral selective reagent. The buffer solution was degassed by ultrasound for 40 minutes, then passed through a 0.45 μm microporous filter membrane, and then degassed by ultrasound for 20 minutes. . The theanine sample was prepared with deionized water at a concentration of 0.1 mg / mL. The instrument used in the experiment was the P / ACE MDQ capillary electrophoresis system of Beckman Company. The parameters of the capillary electrophoresis conditions were: separation voltage 20KV; Sample 3kPa×2s; UV detection wavelength 280 nm; positive electrode into theanine sample, negative electrode detection. The electrophoresis of theanine enantiomers is shown in figure 1 .

Embodiment 2

[0019] Borate solution with a pH value of 12 was used as the buffer solution, and 30 mmol / L hydroxyethyl-β-cyclodextrin was added as the chiral selective reagent. Ultrasonic degassing for 20min. The theanine sample was prepared with deionized water at a concentration of 1.0 mg / mL. The instrument used in the experiment was the P / ACE MDQ capillary electrophoresis system of Beckman Company. The parameters of the capillary electrophoresis conditions were: separation voltage 30KV; Sample 3kPa×2s; UV detection wavelength 280 nm; positive electrode into theanine sample, negative electrode detection. The electrophoresis of theanine enantiomers is shown in figure 2 .

Embodiment 3

[0021] Borate solution with a pH value of 12 was used as the buffer solution, and 25 mmol / L glucosyl-β-cyclodextrin was added as the chiral selective reagent. Degas for 20min. The theanine sample was prepared with deionized water at a concentration of 2.0 mg / mL. The instrument used in the experiment was the P / ACE MDQ capillary electrophoresis system of Beckman Company, and the conditions and parameters of the capillary electrophoresis were the same as those in Example 1. The electrophoresis of theanine enantiomers is shown in image 3 .

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Abstract

The invention discloses a method for efficiently separating and detecting theanine enantiomer by capillary electrophoresis, belonging to the technical field of separation and detection of enantiomers. The method determines the chiral separation environment required by separation of theanine, a borate buffer liquid system with pH value of 10 to 12 is selected, and a chiral selecting reagent is added in the buffer liquid. A theanine sample is prepared, the concentration is 0.1 to 2.0mg / mL, and the theanine enantiomer is separated on baseline in a condition of 15-30kV separation voltage of capillary electrophoresis. The method has good separating efficiency, no pollution and low cost, and is applicable to analysis and detection of theanine enantiomer.

Description

technical field [0001] The invention discloses a method for separating and detecting theanine enantiomers by high-efficiency capillary electrophoresis, which belongs to the technical field of enantiomer separation and detection. Background technique [0002] The resolution and detection of enantiomers was once considered to be one of the most difficult tasks in the separation discipline. Traditional resolution methods include fractional crystallization, microbial and enzyme digestion, etc., but these methods are difficult to separate and measure trace amounts. In recent years, high-efficiency capillary electrophoresis technology has developed rapidly in the detection of enantiomeric separation. This technology has the following advantages: ① There is basically no column pollution problem; ② Low experimental cost and low consumption; ③ Fast analysis speed and high separation efficiency; ④Multiple operating modes. Based on the above advantages, chiral separation by capillary...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
Inventor 朱松李玥王洪新陈培培丁绍东戴军陈尚卫
Owner JIANGNAN UNIV
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