Method for preparing novel mixing artificial blood vessel

An artificial blood vessel and a new type of technology are applied in the field of preparation of new hybrid artificial blood vessels, which can solve the problems of difficult ingrowth of vascular endothelial cells.

Inactive Publication Date: 2011-06-22
THE STOMATOLOGIAL HOSPITAL OF ZHEJIANG UNIV SCHOOL OF MEDICINE
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AI Technical Summary

Problems solved by technology

With the increasing development of tissue engineering, some of them are perforated by the scaffold material, which is beneficial to use the surrounding capillary endothelial cells to grow into the intima layer through the micropores and cover the inner surface; there are also various degradable coatings to reduce the The adhesion of platelets and blood cells, and hope that as the coating is gradually degraded, endothelial cells will gradually crawl and cover, but because the surrounding fibrous connective tissue grows rapidly and closes the pores, it is difficult for vascular endothelial cells to grow into

Method used

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Embodiment Construction

[0005] 1 Treatment of artificial blood vessels The artificial blood vessels with an inner diameter of 3 mm and a length of 4 cm were treated with 75% medical alcohol and 0.01% fibroncetin coating, dried, packaged, sterilized, and set aside.

[0006] 2 Cell culture and subculture The vascular endothelial cell line and vascular smooth muscle cell line were respectively inoculated in the medium of IMDM+10% fetal bovine serum+100IU / ml penicillin+100μg / ml streptomycin for culture, digested with trypsin, and followed 1 : 2 ratio for subculture, subculture once every 4-5 days, and freeze the cells of passage 4-8 for future use.

[0007] 3 Preparation of mixed artificial blood vessels Collect the cultured vascular smooth muscle cell suspension, 6 / cm 2 Inject the prepared artificial blood vessel with a syringe, replace the culture medium every other day, and culture it statically for a week; use the same method to inoculate 2×10 6 / cm 2 The density of vascular endothelial cells for...

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Abstract

The present invention discloses a method for preparing a novel mixing artificial blood vessel, which relates to a surface treatment of artificial blood vessel materials, and the object is to improve the adhesiveness of vascular smooth muscle cells and vascular endothelial cells. According to the present researches, the endothelialization of artificial blood vessel is considered a new direction for researching, but the monolayer endothelial cells on the inner surface of the artificial blood vessel are easy to partly lose under shearing force. According to the invention, 75% medical alcohol is instilled to increase the permeability of the artificial blood vessel and 0.01% fiber adhesion protein coating is used to increase the adhesiveness, in order to increase the adhesiveness of the blood vessel endothelial cells and the surface of the artificial blood vessel. Besides, blood vessel smooth muscle cells and blood vessel endothelial cells are respectively inoculated on the inner surface of the artificial blood vessel to construct an artificial blood vessel with better organism simulation. The method for preparing a novel mixing artificial blood vessel provided by the invention establishes the foundation for the subsequent development and exploitation of organization engineering artificial blood vessel having compositions and functions similar to a physiological artery.

Description

technical field [0001] The invention relates to a preparation method of a novel mixed artificial blood vessel, and aims to improve the adhesion ability of vascular smooth muscle cells and vascular endothelial cells to the artificial blood vessel, and increase the long-term patency rate of the artificial blood vessel after transplantation. Background technique [0002] There are hundreds of thousands of vascular grafts in the world every year. Generally, autologous arteries and autologous veins are used as sources. Due to limited sources, they are greatly restricted clinically. Therefore, the development and manufacture of medical artificial blood vessels has very important practical significance. Large-caliber artificial blood vessels have made breakthroughs in research and have been widely used clinically, while small-caliber artificial blood vessels (diameter <6mm) cannot be effectively resolved due to thrombus, intimal hypertrophy, and biocompatibility. At present, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61F2/06A61L27/38A61L27/34
Inventor 樊立洁
Owner THE STOMATOLOGIAL HOSPITAL OF ZHEJIANG UNIV SCHOOL OF MEDICINE
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