Preparation method for leavening special for goat milk yoghourt
A technology of starter and yogurt, which is applied in the field of preparation of special starter for goat milk yogurt, which can solve problems such as poor coagulation, easy whey precipitation, and long curdling time
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Embodiment 1
[0025] 1. Isolation and purification of lactic acid bacteria
[0026] Separation and purification of Lactobacillus bulgaricus and Streptococcus thermophilus from commercial milk yoghurt by streaking on the plate, the separation and purification method is as follows: use skim milk powder and water to prepare skim milk with a mass fraction of 12%, and sterilize at 115°C for 20 minutes , put the commercial milk yogurt into the sterilized skim milk, the commercial milk yogurt is 2% of the 12% skim milk, fermented to the curd at 37°C; Mark the MRS solid medium and M17 solid medium. MRS solid medium is cultured at 37°C under anaerobic conditions for 2 to 3 days, and M17 solid medium is cultured at 37°C for 2 to 3 days in the atmosphere, and the morphology is selected. The single colonies with the same number and the largest number were stained by Gram, and the characteristics of the bacteria were observed under the oil microscope. The colonies with bacillus characteristics were cult...
Embodiment 2
[0040] In step 2 of the multiplication and cultivation of lactic acid bacteria of the present embodiment, carrot juice, tomato juice and calcium carbonate are added to the MRS liquid medium, the amount of carrot juice added is 10% of the volume of the MRS liquid medium, and the amount of tomato juice added is MRS 12.5% of the volume of the liquid medium, and the amount of calcium carbonate added is 0.5% of the mass of the MRS liquid medium; carrot juice, tomato juice and calcium carbonate are added to the M17 liquid medium, and the amounts of carrot juice and tomato juice are both M17 7.5% of the volume of the liquid medium, and the amount of calcium carbonate added is 0.7% of the quality of the M17 liquid medium, and the other steps of this step are the same as in Example 1. The other steps are the same as in Example 1, and a special starter for goat milk yogurt is prepared.
Embodiment 3
[0042] In the proliferation culture step 2 of the lactic acid bacteria of the present embodiment, carrot juice, tomato juice and calcium carbonate are added in the MRS liquid medium, the addition of carrot juice is 12.5% of the volume of the MRS liquid medium, and the addition of tomato juice is MRS 10% of the volume of the liquid medium, the amount of calcium carbonate added is 0.7% of the quality of the MRS liquid medium; carrot juice, tomato juice and calcium carbonate are added to the M17 liquid medium, and the amount of carrot juice added is the volume of the M17 liquid medium 12.5%, the addition of tomato juice is 10% of the volume of the M17 liquid medium, the addition of calcium carbonate is 0.5% of the quality of the M17 liquid medium, and the other steps of this step are the same as in Example 1. The other steps are the same as in Example 1, and a special starter for goat milk yogurt is prepared.
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