Unlock instant, AI-driven research and patent intelligence for your innovation.

Reagent and method for stabilizing alkaline phosphatase or marker of alkaline phosphatase

A stabilizer and phosphatase technology, applied in biochemical equipment and methods, enzyme stabilization, enzymes, etc., can solve problems such as stable preservation of alkaline phosphatase, and achieve improved stability, extended shelf life, and improved product quality. Effect

Active Publication Date: 2015-06-03
SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD
View PDF4 Cites 9 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Likewise, the patent does not address the stable preservation of alkaline phosphatase in solution

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Reagent and method for stabilizing alkaline phosphatase or marker of alkaline phosphatase
  • Reagent and method for stabilizing alkaline phosphatase or marker of alkaline phosphatase
  • Reagent and method for stabilizing alkaline phosphatase or marker of alkaline phosphatase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Alkaline phosphatase was added to the stabilizer solution described below to prepare an enzyme solution with a final concentration of 5ug / mL, and placed in a constant temperature and humidity chamber at 37°C for accelerated testing. Taking the enzyme solution as a sample, the remaining activity ratio of alkaline phosphatase in the solution (the ratio of the activity after the accelerated test to the activity after preparation) was determined by using the Mindray automatic biochemical analyzer BS300 and the matching alkaline phosphatase assay kit.

[0065] Prepare the solution according to the following formula:

[0066] BSA 1% (g / 100ml)

[0067] MgCl 2 1mM

[0068] Proclin 300 0.05% (g / 100ml)

[0069] Tris 50mM

[0070] Adjust pH with hydrochloric acid to pH 7.5

[0071] As can be seen from the results in Table 1, in the stabilizer of this example, the alkaline phosphatase was accelerated, and after one week, 26% of the enzyme activity remained, and n...

Embodiment 2

[0075] Alkaline phosphatase was added to the stabilizer solution described below to prepare an enzyme solution with a final concentration of 5ug / mL, and placed in a constant temperature and humidity chamber at 37°C for accelerated testing. Taking the enzyme solution as a sample, the remaining activity ratio of alkaline phosphatase in the solution (the ratio of the activity after the accelerated test to the activity after preparation) was determined by using the Mindray automatic biochemical analyzer BS300 and the matching alkaline phosphatase assay kit.

[0076] Prepare the solution according to the following formula:

[0077] BSA 1% (g / 100ml)

[0078] ZnCl 2 , 0.1mM

[0079] NaCl 8g / L

[0080] MgCl 2 1mM

[0081] Proclin 300 0.05% (g / 100ml)

[0082] Tris 50mM

[0083] Adjust pH with hydrochloric acid to pH 7.5

[0084] As can be seen from the results in Table 2, in the stabilizer of this example, the alkaline phosphatase was accelerated, and the enzyme acti...

Embodiment 3

[0088] Alkaline phosphatase was added to the stabilizer solution described below to prepare an enzyme solution with a final concentration of 5ug / mL, and placed in a constant temperature and humidity chamber at 37°C for accelerated testing. Taking the enzyme solution as a sample, the remaining activity ratio of alkaline phosphatase in the solution (the ratio of the activity after the accelerated test to the activity after preparation) was determined by using the Mindray automatic biochemical analyzer BS300 and the matching alkaline phosphatase assay kit.

[0089] Prepare the solution according to the following formula:

[0090] BSA 1% (g / 100ml)

[0091] ZnCl 2 , 0.1mM

[0092] NaCl 100g / L

[0093] Casein 1% (g / 100ml)

[0094] Gelatin 1% (g / 100ml)

[0095] MgCl 2 1mM

[0096] Proclin 300 0.05% (g / 100ml)

[0097] Tris 50mM

[0098] Adjust pH with hydrochloric acid to pH 7.5

[0099] As can be seen from the results in Table 3, in the stabilizer of this example...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present disclosure relates to stabilizers for alkaline phosphatase or conjugates thereof, a process for preparing a stabilizer, and a method for stabilizing alkaline phosphatase or conjugates thereof with a stabilizer. The present disclosure also relates to a reagent of alkaline phosphatase or conjugates thereof as well as to a process for preparing the same. In another aspect, the present disclosure relates to a kit comprising the stabilizers disclosed herein and alkaline phosphatase or conjugates thereof. The stabilizer disclosed herein can stabilize alkaline phosphatase or conjugates thereof for a prolonged period of time, extending their shelf-life.

Description

technical field [0001] The present invention relates to enzyme stabilizers. Specifically, the present invention relates to a stabilizer for alkaline phosphatase or a marker thereof, and a method for stabilizing alkaline phosphatase or a marker thereof using the stabilizer comprises the stabilizer and alkaline phosphatase or a marker thereof reagents. Background technique [0002] Alkaline phosphatase (alkaline phosphatase, AP) is an enzyme that widely exists in animals, plants, microorganisms, etc. Alkaline phosphatase, which is frequently used, is extracted from calf intestinal mucosa and Escherichia coli and consists of multiple isozymes. In an appropriate buffer, alkaline phosphatase can catalyze the hydrolysis of nitrophenyl phosphate (PNP), sodium β-glycerophosphate, naphthyl phosphate, 3-(2-spiroadamantane)-4-methoxy-4 Chromogenic and chemiluminescent substrates containing phosphate groups such as (3-phosphoryloxy)-phenyl-1,2-dioxetane (AMPPD). In view of this, alk...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/96
CPCC12N9/16C12N9/96C12Y301/03001G01N33/535
Inventor 钱纯亘张裕平
Owner SHENZHEN MINDRAY BIO MEDICAL ELECTRONICS CO LTD